The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at 1/4 of the recommended dose can successfully replace cPE.

Ectoparasitic infestation in dairy cattle, the most common being Rhipicephalus (Boophilus) microplus, causes significant loss in production. The control of ectoparasites often occurs incorrectly, resulting in resistance to the active ingredients and residues in milk. To understand of acaricide use in the microregion of Alfenas, Minas Gerais (Brazil), questionnaires were applied to the owners of 100 dairy farms in the region and were selected randomly. Dairy farms were allocated according to production scale: up to 150 liters/day, 151-400 liters/day and more than 400 liters/day. The interviewees were asked about production characteristics, acaricide management and active ingredients. The interview answers were subjected to descriptive analysis. Results showed that all properties, independent of production-scale, used acaricides. The most cited was: Cypermethrin Chlorpyrifos Citronellal (31%), Fluazuron (18.6%) and Fipronil (17%). Among the interviewed, 41,1% use drugs that have restrictions that are not allowed in legislation for use in lactating dairy cows (fluazuron, fipronil, ivermectin). 15% affirmed they weren’t aware of the “withdrawal period” of medication and 73% change in the active ingredient used. In order to revert this antiparasitic drug’s resistance situation and contamination of milk with drug residues, it is necessary to focus on the awareness of milk producers and training of labor.

As tecnologias são incorporadas nas diferentes áreas do nosso cotidiano, este cenário social tecnológico, visto fora das escolas são opostas ao cotidiano escolar, visto que a maioria das escolas brasileiras possuem currículos tradicionais e pouco tecnológicos. Neste contexto, é necessário que a educação se adapte à inovação e a tecnologia, para isso, os professores precisam ser bem formados e incentivados a aplicar metodologias de ensino ativas, atraentes e integradas à tecnologia. O estudo tem como objetivo avaliar a percepção das metodologias ativas por professores que atuam no ensino básico e superior do Estado de Minas Gerais. Os dados foram obtidos das respostas de 485 docentes via formulário eletrônico no ano de 2020. Sobre o conhecimento das metodologias ativas, 82,1% conhecia alguma metodologia, estando mais familiarizados com: seminário (57,2%), aprendizagem baseada em projetos/problemas (51%) e sala de aula invertida (45,7%). Apenas 20,8% foram capacitados durante a graduação e somente 23,3% receberam treinamento da instituição onde trabalham. Dos respondentes, 51,6% não recebem incentivo para utilizar as metodologias e relatam como principais desafios a falta de treinamento (68%), tempo de planejamento (48,5 %), falta de material (48,5%), tempo de aplicação (42,7%) e participação dos alunos (40,2%). Ainda que 79,2% dos respondentes não tenham recebido treinamento durante a graduação, a maioria considera relevante sua inclusão, refletindo a necessidade de modernização dos currículos.

The reintroduction of captive animals to the wild helps restore endangered species, but it risks pathogen transmission, harming wild populations. Such transmission can impact the genetic diversity and long-term viability of these populations. This study assessed parasite diversity and load in captive Pecari tajacu, a species native to the Americas and culturally significant to Brazilian indigenous culture, prior to reintroduction. Samples from 24 peccaries were analyzed for ectoparasites, hemopathogens, and stool parasites with direct and molecular analysis. Findings showed that various parasites were present. Two peccaries (8.3%) were infested by the adult tick Amblyomma sculptum. Six (25.0%) tested positive for Trypanosoma evansi, four (16.7%) for hemobacteria of the family Anaplasmataceae, twelve (50.0%) for hemotropic Mycoplasma, and seven (29.2%) for Leishmania braziliensis. Stool samples indicated multiple parasites, with sixteen (66.7%) peccaries infected by Strongylida order parasites, Spiruridae in three (12.5%), and Ascaris suum in one (4.2%) animal. Cysts of Balantidium sp. were found in twenty (83.3%), Entamoeba polecki in five (20.8%), and Iodamoeba bütschlii in two (8.3%) peccaries. To our current knowledge, this is the first global report of Leishmania braziliensis, Iodamoeba bütschlii, and Entamoeba polecki in P. tajacu, irrespective of the environment, including both captivity and wild conditions. Some of these parasites are common in domestic animals, and others are zoonotic, indicating potential interspecies pathogen transmission.

Modelo do estudo: Relato de Caso. Importância do problema: A fístula aortoentérica primária tem incidência de 0,04% a 0,07% na população, tendo mortalidade de quase 100% se não tratada ou quando associada à hemorragia gastrointestinal. O diagnóstico realizado por endoscopia digestiva alta representa apenas 25% dos casos, sendo a tomografia computadorizada o padrão-ouro para confirmar essa afecção. Comentários: Nesse estudo, relatamos uma afecção rara encontrada na terceira porção do duodeno, com intenso sangramento ativo, sendo diagnosticada por endoscopia digestiva alta e realizado a hemostasia do aneurisma com “clip” endoscópico para controle do sangramento. Em seguida o paciente se estabilizou do ponto de vista hemodinâmico, porém, devido a gravidade do caso, foi encaminhado ao Centro de Terapia Intensiva (CTI) com o propósito de melhores cuidados e realização de Tomografia Computadorizada no dia seguinte, e possível intervenção cirúrgica. Entretanto, o paciente evoluiu com quadro hipertensivo, com ressangramento (11 horas após o diagnóstico), sem sucesso com o tratamento clínico e sem condição para realizar nova hemostasia endoscópica, evoluindo para choque hipovolêmico, seguido de parada cardiorrespiratória e óbito.

As a result of the growing search for healthier eating habits at a more affordable price, the consumption of fish meat has increased in recent years. The city of Pirapora is located in the northern micro-region of the State of Minas Gerais, on the right bank of the Upper Middle São Francisco, where fishing is a traditional activity. With this research, we aim to raise data on the preferences and perceptions of consumers, in the city of Pirapora-MG, on the level of knowledge, regarding the consumption habits and socioeconomic characteristics of consumers. Questions about the knowledge of native, exotic and invasive species were also addressed. With this research, it was possible to draw a diagnosis on the dynamics of consumption and also understand if there is a preference for native species from the São Francisco River, in addition to issues related to the price and access to fish meat, where it was found that the most consumed fish it is the tilapia, even with the presence of the river in the city. However, as shown in the course of the work, in general, more native species are consumed. Thus, as a final outcome, the work helped to better understand the dissemination of information about the importance of native species in the socio-environmental context of communities located on the banks of the São Francisco River.