Objective: This review aimed to analyze and compare the accuracy of eight screening tools for sarcopenia in older Chinese adults according to different diagnostic criteria.Methods: This systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The PubMed, Embase, Web of Science, China National Knowledge Infrastructure (CNKI), and Wanfang databases were searched between the publication of the first expert consensus on sarcopenia in 2010 and April 2023 using relevant MeSH terms. We evaluated the risk bias of the included studies using the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool. The pooled result of sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and plot the summary receiver operating characteristic curve (SROC) were calculated by using a bivariate random-effects model. The accuracies of sensitivity and specificity of the screening tools were compared using the Z-test.Results: A total of 30 studies (23,193 participants) were included, except for calf circumference (CC), Ishii, and Finger-ring Test; Screening tools for sarcopenia in older Chinese adults have consistently shown low to moderate sensitivity and moderate to high specificity. Regional and sex differences affect the accuracy of the screening tools. In terms of sensitivity and specificity, the CC, Ishii, and Finger-ring Test were superior to the other screening tools.Conclusion: The Asian Working Group on Sarcopenia (AWGS) 2019 criteria are more appropriate for the diagnosis of sarcopenia in older Chinese adults. According to the AWGS 2019, CC and Ishii are recommended for sarcopenia screening in older Chinese adults.

Chinese traditional sports are diverse and have rich national cultural connotations. However, with the development of China’s Health Strategy, modern sports such as badminton and basketball occupy most of the people’s time. On the contrary, there is much less exercise for traditional Chinese sports. Therefore, this paper aims to study Chinese traditional sports under China’s Health Strategy and explore its cultural soft power. In this study, participants of all ages in different regions were selected for survey research. Combined with the survey results, the soft power index model of Chinese traditional sports culture is designed. The experimental results showed that for Chinese traditional cultural projects, the cultural soft power index of chui wan was the lowest at 0.568, and the highest was 0.982 in the dragon boat culture soft power index. For each evaluation index, the cultural cognition and cultural attractiveness of Chinese traditional sports were high, but the cultural behavioral power was low. This shows that the soft power model of Chinese traditional sports culture based on the healthy China strategic environment can effectively evaluate the development of traditional Chinese sports, which also can provide suggestions for the development and inheritance of traditional Chinese sports.

Purpose: To determine the impact of resistance training (RT) on phase angle (PhA) in middle-aged and older individuals via meta-analysis, explore effects in subgroups, and identify optimal RT protocol. Materials and methods We searched five databases using predefined criteria, assessed literature quality per Cochrane 5.1 Handbook, and used Revman 5.3 for effect size aggregation, bias assessment, sensitivity analysis, and subgroup analysis. Results: RT improved PhA in middle-aged and older individuals (d = 0.34, 95% CI: 0.27–0.40, P < 0.05). Effective subgroups included Suspension (d = 0.62, 95% CI: 0.33–0.90, P < 0.05), free-weights and machine (d = 0.36, 95% CI: 0.28–0.45, P < 0.05), equipment training (d = 0.24, 95% CI: 0.13–0.36, P < 0.05), and moderate-intensity RT (d = 0.34, 95% CI: 0.27–0.42, P < 0.05). RT was conducted 2-3 times/week (d = 0.20, 95% CI: 0.01–0.38, P < 0.05) or (d = 0.38, 95% CI: 0.30–0.47, P < 0.05). PhA improved after 8 weeks (d = 0.37, 95% CI: 0.23–0.51, P < 0.05), 12 weeks (d = 0.35, 95% CI: 0.26–0.44, P < 0.05), and ≥ 24 weeks (d = 0.26, 95% CI: 0.11–0.41, P < 0.05) of RT in aged and older individuals. Low- and high-intensity RT, elastic band training, and weekly exercises did not significantly improve PhA. Conclusions: RT enhances PhA in middle-aged and older adults. For optimal results, we recommend 2–3 weekly sessions of free weights and machine training lasting at least 8 weeks.

The asb5 gene, a member of the Asb protein subfamily characterized by six ankyrin repeat domains, is highly conserved and comprises two subtypes, asb5a and asb5b, in zebrafish. Our previous research has demonstrated that a deficiency of the asb5 gene significantly impairs early cardiac contractile function, highlighting its close relationship with heart development. Zebrafish asb5 expression was disrupted by both morpholino (MO) antisense oligomer-mediated knockdown and a CRISPR-Cas9 system. A high-throughput RNA-Seq analysis was used to analyze the possible molecular regulatory mechanism of asb5 gene deletion leading to left–right (L-R) asymmetry defects in the heart. Whole-mount in situ hybridization (WISH) was conducted to evaluate gene expression patterns of Nodal signaling components and the positions of heart organs. Heart looping was defective in zebrafish asb5 morphants. Rescue experiments in the asb5-deficiency group (inactivating both asb5a and asb5b) demonstrated that the injection of either asb5a-mRNA or asb5b-mRNA alone was insufficient to rectify the abnormal L-R asymmetry of the heart. In contrast, the simultaneous injection of both asb5a-mRNA and asb5b-mRNA successfully rescued the morphological phenotype. A high-throughput RNA-Seq analysis of embryos at 48 h post fertilization (hpf) revealed that numerous genes associated with L-R asymmetry exhibited expression imbalances in the asb5-deficiency group. WISH further confirmed that the expression of genes such as fli1a, acta1b, hand2, has2, prrx1a, notch1b, and foxa3 were upregulated, while the expression of mei2a and tal1 was downregulated. These results indicated that loss of the asb5 gene in zebrafish led to the disordered development of L-R asymmetry in the heart, resulting in an imbalance in the expression of genes associated with the regulation of L-R asymmetry. Subsequently, we examined the expression patterns of classical Nodal signaling pathway-related genes using WISH. The results showed that the midline barrier factor gene lefty1 was downregulated at early stages in the asb5-deficiency group, and the expression of spaw and lefty2, which are specific to the left lateral plate mesoderm (LPM), was disrupted. This study reveals that the two subtypes of the asb5 gene in zebrafish, asb5a and asb5b, interact and jointly regulate the establishment of early cardiac L-R asymmetry through the Nodal-spaw-lefty signaling pathway.

Background/Purpose: The phase angle is commonly utilized to evaluate the nutritional and health status of middle-aged and older individuals, with higher values indicating better cell quantity and function. Resistance exercise is an efficient method to delay the aging process and decrease the likelihood of falls and mortality. The aims of this study were to 1.) to comprehensively determine the impact of resistance training (RT) on phase angle (PhA) in middle-aged and older individuals through a meta-analysis, 2.) to explore the effects of RT on PhA in healthy and unhealthy groups, including subgroups of middle-aged and older individuals, and to 3.) identify the optimal RT protocol (dose–response relationship) to enhance PhA in this population. Methods: We conducted a systematic search of five databases using predefined criteria. Quality assessment followed the Cochrane Handbook 5.1, and Revman 5.3 facilitated effect size aggregation, bias assessment, sensitivity analysis, and subgroup analysis. Results: RT improved PhA in middle-aged and older individuals (d = 0.34, 95% CI: 0.27–0.40, P < 0.05). Subgroup analysis indicated PhA benefits in unhealthy (d = 0.25, 95% CI: 0.10–0.41, P < 0.05) and healthy groups (d = 0.35, 95% CI: 0.28–0.43, P < 0.05). Suspension (d = 0.62), free-weights/machine (d = 0.36), equipment training (d = 0.24), and moderate-intensity RT (d = 0.34) improved PhA. PhA improved with RT performed twice (d = 0.20) or three times (d = 0.38) weekly. Significant improvements occurred after 8 weeks (d = 0.37), 12 weeks (d = 0.35), and ≥24 weeks (d = 0.26) of RT. Low/high-intensity RT, elastic bands, and weekly exercises did not significantly improve PhA. Conclusion/Discussion: RT can enhance PhA in both healthy and unhealthy middle-aged and older individuals. Optimal results are achieved with 2–3 times per week, moderate-intensity RT, lasting ≥ 8 weeks. Given the limited number of comparisons for some subgroups, the conclusions deduced from the subgroup results should be taken as preliminary. Future studies should be designed on the exercise program to compensate for the limitations of the existing studies. At the same time, the study population should be expanded, and additional studies should be conducted on diseased populations other than patients with obesity and diabetes. In addition, the difference in the effect of RT combined with other exercises versus RT only on PhA and the mechanism of its effect are unclear, and more experimental studies on the level of RT effect combined with other exercises on PhA are recommended.

The Popeye domain-containing protein 1 (Popdc1), also known as Bves, plays a crucial role in maintaining skeletal muscle homeostasis, with its variants leading to limb–girdle muscular dystrophy type R25. Skeletal muscles of patients with the homozygous missense variant of Bves exhibit impaired membrane trafficking, while skeletal muscle fibers in bves^S191F homozygous mutant zebrafish are significantly reduced and disorganized. However, the mechanism by which the absence of bves induces skeletal muscle atrophy remains unclear. In this study, we discovered a novel mechanism whereby bves deficiency drives skeletal muscle atrophy by disrupting mitochondrial structure and function. Our findings indicate that bves knockout leads to a significant decrease in zebrafish’s ability to swim, atrophy of skeletal muscle tissue, loss of cell membrane localization signals, and abnormalities in mitochondrial structure and function. After an 8-week intervention of regular aerobic exercise, the symptoms of skeletal muscle atrophy in bves knockout zebrafish were significantly alleviated, and the expression levels of genes and proteins related to mitochondrial were effectively rescued. These findings establish a connection between bves deficiency-induced disruption of mitochondrial structure and function and the onset and progression of skeletal muscle tissue atrophy symptoms, thereby laying a molecular foundation for exercise rehabilitation strategies in atrophic myopathy.