Chemical and mechanical root canal debridement are the primary methods used in endodontic therapy to remove all dead tissue, bacteria, and microbial byproducts from the canal. Sodium hypochlorite, a powerful organic tissue dissolver with a broad spectrum of antibacterial properties, is an excellent choice for disinfecting surfaces. Chelating agents, on the other hand, may be used to remove the inorganic components found on the smear layer. This irrigation method is capable of removing the smear layer; however, it is less effective in the apical third. While using irrigant activation devices, irrigating solutions need to be in direct contact with the whole root canal wall surfaces, especially in the apical portions of tiny root canals. The role of irrigants is extremely important because they help not only to clean the canal but also to allow the seepage of the medicaments into the canal system. Thus, the canal needs to be healthy before the obturation procedure. Nowadays, many irrigants have been studied and hence compilation of the various available sources and their effect has to be studied both in vivo and in vitro. The correlation between the irrigants and the canal cleanliness is of utmost importance, as the success of endodontic root canal treatment depends on its proper activation and characteristics. Hence, this review incorporates the current use of various irrigating solutions and their advantages and disadvantages. In the future, endodontists may employ the novel irrigants and irrigant activation devices that were discovered in this study.

The novel coronavirus (COVID-19) is an extremely contagious respiratory disease. It poses a serious threat to healthcare workers which may cause substantial degree of psychological distress. This study aimed to evaluate the level of fear and anxiety among healthcare workers. A cross sectional study was designed to collect data from healthcare workers in Saudi Arabia using an online questionnaire. A non-probability convenient sampling technique was used to collect the data from 1st August to 10th August 2020 with the exclusion of non-healthcare worker and general public. A 5-point Likert scale was used to evaluate the level of fear and anxiety. The data was analyzed by using Spss version 23 .The descriptive data were represented as frequencies and percentage. Multinomial logistic regression analysis was applied to control the confounding factors and assess the relationship between variables. Data analyzed 737 participants revealed that 10.7%, 73.5%, and 15.7% of HCWs had a mild, moderate, and severe degree of fear and anxiety respectively. The multinomial logistic regression analysis revealed that gender and specialty of the participants were significantly associated with moderate and severe anxiety level. This study demonstrates that two-third of the healthcare workers had a moderate risk of fear and anxiety. However, females participants found to have elevated fear and anxiety level. Social distancing has a major impact on social well-being therefore its important to address social support through a policy. Further studies are needed to explore the mental health condition of healthcare workers in order to develop evidence based strategies.

The ideal root end filling material should form a tight seal in the root canal by adhering to the cavity walls. Several materials have been used for root end filling. The present study aims to find out and compare the bioactivity of Neo MTA Plus, Pro Root MTA White, BIODENTINE & glass ionomer cement as root end filling materials using 1% methylene blue as tracer. Materials and methods: 80 extracted human permanent maxillary anterior teeth were used in the study. They were divided into four groups. Specimens were sectioned transversely in the cervical area to separate the crown from the root. The root canal was obturated with gutta percha and zinc oxide eugenol sealers. Thereafter, each sample was resected apically by removing 3 mm of the apex and filled with different materials. Samples were kept in buffering solution at 37 °C until the recommended evaluation periods. The specimens were then suspended in 1% methylene blue for 24 h, prior to the analysis. The teeth were then sectioned, and dye penetration was examined, photographed, and evaluated under a stereomicroscope. Results: Vertical dye penetration showed significant differences across different groups. The minimum dye penetration was seen in Neo MTA plus followed by BIODENTINE, Pro Root MTA and maximum in GIC. There was no significant difference in dye penetration between Neo MTA plus and BIODENTINE both at fifteen days and one-month intervals. Conclusion: The present study suggests Neo MTA plus and BIODENTINE should be the preferred material for root end filling.

Nanodiamonds (NDs) are being used for a range of applications such as drug delivery, tissue regeneration and gene therapy. Although the potential of these remarkable materials has been investigated for dental applications, to date there is no published review to summarize the conducted studies. Due to unique properties such as target cell specificity, nanometer size and fluorescence, NDs have been found useful for various applications in dentistry. Main applications of NDs in dentistry include guided tissue regeneration, reinforcement of polymers and drug delivery to treat infections and cancers. Researchers have suggested that NDs can be used as bioactive or antibacterial dental implant coatings. However, to date, the research conducted on their biocompatibility is limited and inconclusive. Hence, substantially more in vitro and in vivo studies are required to envisage the future of NDs in dentistry. It is hoped that in the next decade, these promising materials will find a variety of routine applications in clinical dentistry.

Objective: The aim of the current study was to carry out a preliminary validation of devices for standardized collection of whole mouth fluid (WMF) in comparison to the passive drooling method for protein analysis in healthy subjects. Materials and Methods: A carefully designed sample collection/pretreatment protocol is crucial to the success of any saliva proteomics project. In this study, WMF was collected from healthy volunteers (n = 10, ages: 18–26 years). Individuals with any oral disease were excluded from the study group. In our study, we evaluated the following collection methods; the classical passive drooling method (unstimulated whole saliva) and standardized tools for saliva collection (Pure·SAL™, and RNAPro·SAL™) from Oasis Diagnostics^® Corporation (Vancouver WA, USA). For estimation of protein levels, we used the bicinchoninic acid assay and protein assay kit (Thermo Fisher). The two-dimensional gel electrophoresis sample analysis was carried out for the estimation of proteins in one of the samples. Results: When gels were compared, the difference was seen in the resolution of spots. Protein spots were fading from high- to low-molecular weight masses. Hence, advanced devices in comparison to spitting method resulted in much clearer protein spots which in turn prove the validation of devices. Conclusions: In this study, we concluded that protein extraction could be possible by both methods such as passive drooling method and through advanced saliva collection devices (Pure·SAL™ and RNAPro·SAL™).