N-acyl-homoserine lactones (AHLs), a well-described group of quorum sensing molecules, may modulate plant defense responses and plant growth. However, there is limited knowledge regarding the defense responses of non-model crops to AHLs and the mechanism of action responsible for the modulation of defense responses against microbial pathogens. In the present study, long-chain N-3-oxo-tetradecanoyl-l-homoserine lactone (oxo-C14-HSL) was shown to have a distinct potential to prime cucumber for enhanced defense responses against the biotrophic oomycete pathogen Pseudoperonospora cubensis and the hemibiotrophic bacterium Pseudomonas syringae pv. lachrymans. We provide evidence that AHL-mediated enhanced defense against downy mildew disease is based on cell wall reinforcement by lignin and callose deposition, the activation of defense-related enzymes (peroxidase, β-1,3-glucanase, phenylalanine ammonia-lyase), and the accumulation of reactive oxygen species (hydrogen peroxide, superoxide) and phenolic compounds. Quantitative analysis of salicylic acid and jasmonic acid, and transcriptional analysis of several of genes associated with these phytohormones, revealed that defense priming with oxo-C14-HSL is commonly regulated by the salicylic acid signaling pathway. We also show that treatment with short- (N-hexanoyl-l-homoserine lactone) and medium-chain (N-3-oxo-decanoyl-l-homoserine lactone) AHLs promoted primary root elongation and modified root architecture, respectively, resulting in enhanced plant growth.

This study introduces a simple and cost-effective method for the indirect determination of field capacity (FC) in soil, a critical parameter for soil hydrology and environmental modeling. The relationships between FC and soil moisture constants, specifically maximum capillary water capacity (MCWC) and retention water capacity (RWC), were established using undisturbed soil core samples analyzed via the pressure plate method and the “filter paper draining method”. The aim was to reduce the time and costs associated with traditional FC measurement methods, as well as allowing for the use of legacy databases containing MCWC and RWC values. The results revealed the substantial potential of the “filter paper draining method” as a promising approach for indirect FC determination. FC determined as soil water content at −33 kPa can be effectively approximated by the equation FC33 = 1.0802 RWC − 0.0688 (with RMSE = 0.045 cm³/cm³ and R = 0.953). FC determined as soil water content at −5 or −10 kPa can be effectively approximated by both equations FC5 = 1.0146 MCWC − 0.0163 (with RMSE = 0.027 cm³/cm³ and R = 0.961) and FC10 = 1.0152 MCWC − 0.0275 (with RMSE = 0.033 cm³/cm³ and R = 0.958), respectively. Historical pedotransfer functions by Brežný and Váša relating FC to fine particle size fraction were also evaluated for practical application, and according to the results, they cannot be recommended for use.

The positive influence of?surface compost application without incorporation on?soil physical properties is?known but remains underexplored. This study evaluated the effects of?surface-applied stable and mature compost on?basic soil physical an...