Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection.
15 December 2013 in International Journal of Plant Breeding and Genetics
Ghana has great potential to produce rice for local consumption, however, the average rice produced barely meets half of the country's consumption needs. Focus group discussions (FGDs) and surveys were held within the coastal lowland rice production belt in Ghana. The FGDs were held at Okyereko and Afife followed by surveys in the two communities in addition to Dawenya and Ashaiman. The objectives were to assess the production challenges faced by farmers in the four communities and determine the rice traits preferred by the farmers. Twenty farmers were involved in each of the FGDs while 227 respondents were selected through convenient sampling for the interviews. Sixty-nine percent of respondent farmers were male, 53% were above 50 years while 44% had varied levels of education. Farmers preferences mainly related to marketable traits such as aroma (87%), taste (83%) and yield potential (78%). There was special preference for Jasmine 85 (62.8%) and Togo Marshal l (25%), an indication of the high adoption of aromatic rice varieties in the study areas. Challenges such as securing credit, input supplies, soil salinity and ageing work force were matters of grave concern to respondent farmers. Given the importance of rice cultivation in the study areas to Ghana’s quest to achieve self-sufficiency in rice, targeted and coordinated support from relevant governmental and non-governmental organizations is needed to sustain a higher level of production in the study areas.
15 April 2013 in International Journal of Plant Breeding and Genetics