Infrastructure plays the largest role in the amount of annual emissions, so much so that investments promoted in the European Union must be subjected to a careful assessment of the sustainability of projects. The current landscape for assessing the sustainability of infrastructure is varied and complex. Considering the object of the assessment methodologies (such as the Environmental Impact Assessment or the Ecological Management System) and specific tools such as Envision, there is a shift from the infrastructure in itself and the company’s actions to promoting sustainable development. This article introduces a methodology to examine how tools used in environmental impact assessments of transport infrastructure projects, regardless of the actor implementing them, align with different sustainable development objectives. Moreover, it identifies the Sustainable Development Goals (SDGs) as a reference point that can be used in estimating the validity of these instruments. This paper also validates the methodology proposed in our study, by comparing the results obtained on the Envision model with those obtained from its application in a case study regarding the Terzo Valico dei Giovi, a railway infrastructure in Italy. The article shows that although the final target is in many respects the same, the nuances with which actors pursue sustainability through the different instruments vary.