L -Methionine -lyase (MGL) is a pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyzes -elimination of L -methionine. The crystal structure of MGL from Citrobacter freundii has been determined at 1.9 Å resolution. The spatial fold of the protein is similar to those of MGLs from Pseudomonas putida and Trichomonas vaginalis. The comparison of these structures revealed that there are differences in PLP-binding residues and positioning of the surrounding flexible loops.