Lung squamous cell carcinoma (LUSC) is a common histologic subtype of non-small cell lung cancer with a poor prognosis. RNA-binding proteins (RBPs) are key modulators in the posttranscriptional regulation and RBP alterations are commonly found in various cancer types. However, its roles in predicting the tumorigenesis and prognosis have not been identified in LUSC. To identify the roles of RBPs in the tumorigenesis and prognosis of LUSC, the RNA sequencing data of patients with LUSC were retrieved from The Cancer Genome Atlas (TCGA) databases. The differential expressed genes (DEGs) were evaluated and identified. The intersection of manually curated RBPs and tumorigenesis-related DEGs was filtered to the univariate Cox regression analysis. The intersection genes with prognostic value were defined as prognostic RNA-binding protein genes (PRBPGs). Based on them, the predicted model was constructed. Its accuracy was tested by the area under the curve (AUC) of the receiver operator characteristic curve and the risk score. In addition, to explore the key regulatory network, the relationship among PRBPGs, target RNA, and absolute quantification of 50 hallmarks of cancer was also identified by Pearson correlation analysis. A total of 311 genes were filtered as the intersection of 1542 manually curated RBPs and tumorigenesis-related DEGs and the results revealed 17 PRBPGs. Based on them, we constructed the predict model with a relatively high accuracy (AUC: 0.739). The Kaplan–Meier survival curve showed the significant prognostic value of risk score ( p < 0.001). Moreover, we uncovered the regulatory networks of PHF5A-TOMM22-oxidative phosphorylation, TLR3-CTSO inflammation-related pathway, SECISBP2L-targeted RNA (ADGRF5, TGFBR2, CD302, AC096921.2, AHCYL2, RPS6KA2, SLC34A2, and SFTPB) angiogenesis, and SECISBP2L-AKAP13 signaling (DNA repair, MTORC1 signaling, and MYC targets). The regulation mechanisms and cellular location of key PRBPGs were validated by assay for targeting accessible chromatin with high-throughput sequencing and single-cell RNA sequencing. Our study identifies PRBPGs as reliable indexes in predicting the tumorigenesis and prognosis of patients with LUSC and provides a well-applied model for predicting the overall survival for patients with LUSC. Besides, we also identified the regulatory network among PRBPGs, target RNA, and cancer gene sets in mediating the LUSC tumorigenesis.

Objective To analyze the clinical characteristics, imaging, laboratory examination，treatment and prognosis of Chlamydia psittaci (C. psittaci) pneumonia, so as to improve the early diagnosis and treatment of this disease. Methods A retrospective analysis obtained from the First Affiliated Hospital of Zhengzhou University from September 2019 to June 2022 was performed. The epidemiological characteristics, clinical manifestations, laboratory tests, imaging features, treatment and prognosis of 22 patients with C. psittaci pneumonia confirmed by of Metagenomic next-generation sequencing (mNGS). Results Among the 22 patients, 13 (59.09%) were males and 9 (40.91%) were females, ranging in age from 25 to 81 years old, with an average age of (59.73±12.04) years. 14 patients had a history of contact with poultry or birds. The common symptoms were fever (22/22, 100%), cough (15/22, 68%), fatigue (11/22, 50%), dyspnea (9/22, 41%), expectoration (7/22, 32%), headache (9/16, 56%).The median number of white blood cells (WBC)was within the normal range, C-reactive protein (CRP), procalcitonin (PCT), Creatine kinase (CK), Lactate dehydrogenase (LDH), Aspartate aminotransferase (AST), D-dimer and Fibrinogen (FIB) were significantly increased. CT scan showed consolidation (22/22,100%), Multilobular distribution (11/22, 50%) and pleural effusion (6/22, 27%).According to CURB-65 score, 22 patients were divided into severe group and non-severe group. Compared with non-severe patients, CRP increased more significantly in severe patients (P<0.01).The percentage of neutrophils, neutrophil lymphocyte ratio (NLR)，PCT, LDH and CK increased significantly in severe patients (P<0.05), while the platelet（PLT）decreased significantly (P<0.05). All 22 patients were diagnosed by mNGS, and the time from onset to diagnosis was 5 days. Doxycycline regimen is effective and the temperature of 15 patients (68.2%) returned to normal within 3 days. Among the 22 patients, 5 patients received invasive mechanical ventilation, including 2 patients treated with Veno-veno extracorporeal membrane oxygen (VV-ECMO) and 2 patients developed septic shock and required vasoactive drug treatment. These five patients were Death at follow-up after automatic discharge. Of the remaining 17 patients, 1 received non-invasive mechanical ventilation and 3 received high-flow humidified oxygen therapy. The remaining 17 patients recovered. Conclusions C. psittaci pneumonia is an atypical community-acquired pneumonia (CAP) caused by C. psittaci infection. The characteristic clinical manifestations of C. psittaci pneumonia are high fever, fatigue, lobar pneumonia, normal WBC count and elevated inflammatory indicators. The symptoms of severe patients are not typical, but laboratory results can provide some clues. mNGS technology can detect C. psittaci early, reduce unnecessary antibiotic use. The treatment effect of doxycycline is significant, and most patients have a good prognosis, but if the patient's condition is severe and invasive mechanical ventilation is required, the prognosis is poor. Fundprogram: Joint project of Medical Science and Technology Project of Henan Province in 2020(LHGJ20200316)