To study the application value of sweet cherry leaves before abscission, a supercritical carbon dioxide (SFE-CO₂) extraction method was established for sweet cherry (Prunus avium L.) leaves.

This study was designed to investigate the impact of blood tumor mutational burden (bTMB) on advanced NSCLC in Southwest China. The relationship between the tTMB estimated by next-generation sequencing (NGS) and clinical outcome was retrospectively analyzed in tissue specimens from 21 patients with advanced NSCLC. Furthermore, the relationship between the bTMB estimated by NGS and clinical outcome was retrospectively assessed in blood specimens from 70 patients with advanced NSCLC. Finally, 13 advanced NSCLC patients were used to evaluate the utility of bTMB assessed by NGS in differentiating patients who would benefit from immunotherapy. In the tTMB group, tTMB ≥ 10 mutations/Mb was related to inferior progression-free survival (PFS) (hazard ratio [HR], 0.30; 95% CI, 0.08-1.17; log-rank P = 0.03) and overall survival (OS) (HR, 0.30; 95% CI, 0.08-1.16; log-rank P = 0.03). In the bTMB group, bTMB ≥ 6 mutations/Mb was associated with inferior PFS (HR, 0.32; 95% CI, 0.14-1.35; log-rank P < 0.01) and OS (HR, 0.31; 95% CI, 0.14-0.7; log-rank P < 0.01). In the immunotherapy section, bTMB ≥ 6 mutations/Mb was related to superior PFS (HR, 0.32; 95% CI, 0.14-1.35; log-rank P < 0.01) and objective response rates (ORRs) (bTMB < 6: 14.2%; 95% CI, 0.03-1.19; bTMB ≥ 6: 83.3%; 95% CI, 0.91-37.08; P = 0.02). These findings suggest that bTMB is a validated predictive biomarker for determining the clinical outcome of advanced NSCLC patients and may serve as a feasible predictor of the clinical benefit of immunotherapies (anti-PD-1 antibody) in the advanced NSCLC population in Yunnan Province.

Moso bamboo (MB), carbonized moso bamboo (CMB), natural color bamboo scrimber (NBS), and dark color bamboo scrimber (DBS) were subjected to Poria vaporaria (PP), Gloeophyllum trabeum (GT), Coriolus versicolor (CV) and Trametes versicolor (TV) in laboratory experiments. The differences in surface chromaticity and mass loss of four samples before and after decay were compared. The results showed that the decay resistance of DBS after four wood rot fungal infections reached grade I, and DBS had the best decay resistance among the four samples. MB had the worst decay resistance among the four samples. After being infected by four kinds of wood rot fungi, the color of MB and NBS changed significantly, as the brightness decreased significantly. The red-green axis color index and yellow–blue axis color index of MB and NBS infected by PP, GT, CV, and TV showed significant increases compared to those of untreated samples. The chroma values of MB and NBS increased significantly after the decay test. SEM analysis showed that after the brown-rot test, the hyphae of GT entered the MB vessels and the parenchyma cells through the pits in the vessel, causing the cell structure to be destroyed.