The aim of this study was to use landscape grass from species-rich orchards for biogas production, thus preserving these very valuable areas for future generations. Since these grass clippings have high lignocellulose content, the substrate has to be pretreated before being fed into the biogas digester. In this study, three different mechanical treatment processes (cross-flow grinder, ball mill and a mounted mower) were investigated and compared with untreated grass clippings. Chemical composition, specific methane yield, degradation kinetics and microscopic images were analyzed. In order to derive recommendations, the harvesting and pretreatment processes were examined in terms of energy demand, additional methane yield, and suitability of the substrate for use in biogas plants, taking into account conservation aspects. Within the pretreatment process, ball milling leads to the highest significant increase in specific methane yield of up to 5.8% and the fastest gas formation kinetics (lag time λ_BM: 0.01 ± 0.0 d; duration to reach half of total gas production ½M(x)_BM: 5.4 ± 0.2 d) compared to the untreated variant (λ_UT: 1.02 ± 0.2 d; ½M(x)_UT: 6.5 ± 0.2 d). A comparison of the energy required for the mechanical disintegration of the substrates with the increased yield of methane during the digestion process shows that the mechanical processing of these substrates appears to be useful. A positive energy balance was achieved for the cross-flow grinder (12.3 kWh t_VS⁻¹) and the ball mill (21.4 kWh t_VS⁻¹), while the Amazone Grasshopper left a negative balance (−18.3 kWh t_VS⁻¹), requiring more energy for substrate pretreatment than was generated as methane surplus. In summary, the pretreatment of landscape management grass is a suitable approach for utilizing agricultural residues efficiently in a biogas plant and thus contributing to sustainable energy production.

Orchard meadows are biodiversity hotspots, as the understory often consists of species-rich lowland hay meadows. Due to the low energy density of the grass, it is not suitable as feed, but the energetic utilisation of cuttings from orchard meadows for biogas production could facilitate the protection of these semi-natural grasslands. Here, lowland hay meadows and extensively used orchards were investigated to assess their potential for anaerobic digestion in biogas plants. Aboveground biomass was harvested weekly from three lowland hay meadows differing in conservation statuses and analysed for cell wall components (aNDF, ADF, and ADL), nutritional values (XF, XL, XP), and methane formation potential by anaerobic digestion. Further, orchard meadows were harvested twice during summer and analysed in the same way. Specific methane yield decreased linearly with cutting dates from 0.325 m³ kg⁻¹_(oDM) to 0.237 m³ kg⁻¹_(oDM). The cumulated area-related methane yields of the orchards ranged from 818 m³ ha⁻¹ to 1036 m³ ha⁻¹. Specific methane yields were linearly correlated with XL, aNDF, ADF, and ADL.