A total of 12 Salmonella isolates belonging to different serovars, viz ., Salmonella enterica serovar Enteritidis ( n = 4), Salmonella enterica serovar Weltevreden ( n = 4), Salmonella enterica serovar Newport ( n = 1), Salmonella enterica serovar Litchifield ( n = 1), and untypeable strains ( n = 2) were isolated from 332 diarrheic fecal samples collected from animals, birds, and humans. Of the two molecular typing methods applied, viz ., repetitive element sequence-based PCR (REP-PCR) and pulsed-field gel electrophoresis (PFGE), PFGE could clearly differentiate the strains belonging to different serovars as well as differentiate between strains of the same serovar with respect to their source of isolation, whereas REP-PCR could not differentiate between strains of the same serovar. Thus, it can be suggested that PFGE is more useful and appropriate for molecular typing of Salmonella isolates during epidemiological investigations than REP-PCR.

Canine Mammary Tumour (CMT) is the most common malignancy of unspayed female dogs and is of potential importance as a model for human breast cancer. Mortality rate in female dogs is three times than that of human females with breast cancer due to lack of detection at an early stage. Therefore it is important to identify candidate biomarkers for canine mammary tumour with potential for disease diagnosis at early stages. Tumour antigen P53 (TP53) is well established marker for human breast cancer studies, however few studies have focussed on its characterization in CMTs. Therefore in this study immunodominant region in TP53 gene was amplified and cloned in prokaryotic expression vector. Further the conditions were optimized for the expression of the recombinant protein. The recombinant protein was purified using Ni-NTA affinity chromatography. The hyperimmune sera were raised in rabbits against the recombinant protein. In the hyperimmune sera, antibodies specific to TP53 were detected by Indirect ELISA, confirming the serological reactivity of the immunodominant region. The antibodies raised against canine TP53 will be useful in developing assays for detecting TP53 antigen in serum of dogs with cancers. Further the purified antibodies can also be utilized for detection of TP53 antigen in CMT tissues.