This study aimed to investigate the relationship between body condition score (BCS) and reproductive recovery in Sarda dairy breed ewes, as well as its association with some hematochemical parameters, focusing on the male effect as a method to induce out-of-season ovulation. The male effect is commonly used in sheep farming as an alternative to hormonal treatments, but its success can be influenced by factors such as BCS. In this experiment, 200 healthy lactating ewes (aged 3–5 years) were divided into four groups based on BCS (2.0, 2.5, 3.0, and 3.5). BCS was measured on a 1–5 scale by touching the amounts of muscling and fat deposition in the loin region. On 15 May, 10 rams of proven fertility were introduced, and removed 50 days later. Blood samples were collected at days 15, 30, and 50 for glucose, cholesterol, triglycerides, total protein, and albumin analysis, along with BCS evaluations. Lambing dates, fertility rates, litter size, and the time from ram introduction to lambing were recorded. The results showed that ewes with a higher BCS (3.0 and 3.5) had improved fertility rates, earlier lambing, and a better overall reproductive performance compared to ewes with a lower BCS (2.0 and 2.5). Higher BCS groups also exhibited higher levels of glucose, cholesterol, and triglycerides. The study concluded that a BCS of 3.0 is optimal for reproductive efficiency in Sarda ewes during the male effect in spring, promoting better reproductive outcomes and animal welfare without the need for hormonal treatments.

This study aimed to evaluate the discrepancies between genetic and visual coat color assignment in the Sarcidano Horse and to elucidate potential reasons. Individual DNA from 90 Sarcidano Horses was used for genetic assignment of coat color to explore the correspondence with individual forms containing phenotypical traits. The MC1R exon 1 and ASIP exon 3 have been genotyped and sequenced to obtain a picture of the coat color distribution in this breed. Surprisingly, once we compared the genetic results with the individual forms reporting the phenotypic data for each subject, a certain degree of non-correspondence between the phenotypic and genetic data in relation to coat color emerged. From the genetic analysis, Chestnuts (n = 58) resulted the most common Sarcidano Horse (n = 58), followed by a quite large number of Blacks (n = 28) and a very small number of Bays (n = 4), whereas phenotypic distribution resulted in 38 Chestnuts, 40 Bays, only 2 Blacks, and 10 Grays (without the possibility of recognizing the true color they carried). Chestnut resulted a very representative coat color, while many horses that visually identified as Bays were genetically Blacks. This discrepancy, that could be due to a variety of individual and external factors, including age, time of year, living situation and dietary condition, suggesting the importance of accurate coat color identification to ensure adequate features registration and reliable prediction of offspring’s coat color.

This study aimed to evaluate the discrepancies between genetic and visual coat color assignment in the Sarcidano Horse and to elucidate potential reasons. Individual DNA from 90 Sarcidano Horses was used for genetic assignment of coat color to explore the correspondence with individual forms containing phenotypical traits. The MC1R exon 1 and ASIP exon 3 have been genotyped and sequenced to obtain a picture of the coat color distribution in this breed. Surprisingly, once we compared the genetic results with the individual forms reporting the phenotypic data for each subject, a certain degree of non-correspondence between the phenotypic and genetic data in relation to coat color emerged. From the genetic analysis, Chestnuts (n = 58) resulted the most common Sarcidano Horse (n = 58), followed by a quite large number of Blacks (n = 28) and a very small number of Bays (n = 4), whereas phenotypic distribution resulted in 38 Chestnuts, 40 Bays, only 2 Blacks, and 10 Grays (without the possibility of recognizing the true color they carried). Chestnut resulted a very representative coat color, while many horses that visually identified as Bays were genetically Blacks. This discrepancy, that could be due to a variety of individual and external factors, including age, time of year, living situation and dietary condition, suggesting the importance of accurate coat color identification to ensure adequate features registration and reliable prediction of offspring’s coat color.