The early loss of dental implants can be avoided with systemic antibiotics, however there are potentially significant side effects. Consequently, the use of local drug administration techniques is necessary to make dental implant therapy more practical. In this study, Y-branched nanotubes were prepared by non-expensive and simple anodization in two steps. Tests were performed to highlight their potential for local antibiotic administration. Y-branched nanotubes were able to incorporate a dose of Tetracycline and ensure its electrochemical stability. The presence of tetracycline significantly enhanced antibacterial efficacy, resulting in an increase of up to 55% for Escherichia coli and Pseudomonas aeruginosa and 50% for Staphylococcus aureus. The comparable antibacterial effects of the nanostructured surfaces highlight the potential of tetracycline in promoting antimicrobial action. Moreover, the addition of tetracycline does not influence the structural, morphological and stability properties of the nanostructured deposited TiO₂ films.