Blue honeysuckle (Lonicera caerulea L.) bears dietary fruits that are rich in bioactive compounds. However, information on the metabolome profiles of honeysuckle varieties grown in Russia is limited. In this study, we employed tandem mass spectrometry to study the metabolome profiles of four L. caerulea varieties (Volhova, Tomichka, Goluboe vereteno, and Amfora) grown in two geographical locations in Russia, i.e., the Russian Far East and St. Petersburg. We observed that the metabolome profiles of the four varieties grown in two locations differ significantly, particularly in the polyphenol’s other compound classes. We were able to identify 122 bioactive compounds in extracts from honeysuckle berries, 75 compounds from the polyphenol group and 47 compounds from other chemical groups. Thirty chemical constituents from the polyphenol group (flavones jaceosidin, cirsiliol, sophoraisoflavone A, chrysoeriol-O-hexoside, flavonols dimethylquercetin-3-O-dehexoside, rhamnocitrin, rhamnetin II, stilbenes pinosylvin, resveratrol, dihydroresveratrol, etc.) and twenty-seven from other chemical groups were identified. The largest number of unique polyphenols is characteristic of the variety Tomichka, the selection of the regional state unitary enterprise “Bakcharskoye”, from the free pollination of L. caerulea, originating in the Primorsky Territory of Russia (L. caerulea subspecies Turczaninow). This genotype has the highest number of similar unique polyphenols, regardless of where it was grown. Blue honeysuckle genotypes originating from Primorsky Krai in Russia can be used in various breeding programs in order to improve and enrich the biochemical composition of fruits. It should also be noted that, regardless of the place of cultivation, the total amount of unique polyphenols remains quite large. Attention should be paid to the Volhova honeysuckle variety, obtained through gamma irradiation of the Pavlovskaya variety (Kamchatka ecotype). This sample is characterized by a stable composition of biologically active substances, regardless of the growing area. These data could support future research on the production of a variety of pharmaceutical products containing ultrapure extracts of L. caerulea.

Blue honeysuckle (Lonicera caerulea) is widespread across the Eurasian continent, mainly in northern latitudes. Its berries are a rich source of biologically active compounds. In this study, plant samples collected in four regions of Russia separated by more than 10,000 km were examined in detail: St. Petersburg, Kamchatka, Magadan and the Far East (Vladivostok). The study was unique in that it covered almost the entire Eurasian continent in northern latitude, which had not been previously presented in other scientific studies. The study revealed the presence of 110 polyphenols and 34 compounds belonging to other chemical groups. In particular, honeysuckle berries were rich in polyphenols, including flavonoids, flavanones, flavanols, flavan-3-ols, anthocyanins, stilbenes, and lignans. The method of tandem mass spectrometry was used to identify biologically active substances from the extracts, which allows obtaining fairly accurate results. The metabolomic composition of L. caerulea berries originating from Kamchatka and Magadan showed the greatest diversity of polyphenols, which is associated with special northern climatic conditions and associated stress factors for plants. The results we obtained provide new data on the composition of the honeysuckle berry metabolome. The wealth of biologically active substances in blue honeysuckle berries can be very interestingly used in the development of both biologically active additives for pharmaceutical use and for the development of functional and specialized nutrition products for various population groups.

A comparative metabolomic study of three varieties of wild Rosa (Rosa acicularis, Rosa amblyotis, and Rosa rugosa) from a Kamchatka expedition (2024) was conducted via extraction with supercritical carbon dioxide modified with ethanol (EtOH), and detection of bioactive compounds was realized via tandem mass spectrometry. Several experimental conditions were investigated in the pressure range 50–350 bar, with the used volume of co-solvent ethanol in the amount of 2% in the liquid phase at a temperature in the range of 31–70 °C. The most effective extraction conditions are the following: pressure 200 Bar and temperature 55 °C for Rosa acicularis; pressure 250 Bar and temperature 60 °C for Rosa amblyotis; pressure 200 Bar and temperature 60 °C for Rosa rugosa. Three varieties of wild Rosa contain various phenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI–ion trap) was applied to detect the target analytes. A total of 283 bioactive compounds (two hundred seventeen compounds from the polyphenol group and sixty-six compounds from other chemical groups) were tentatively identified in extracts from berries of wild Rosa. For the first time, forty-eight chemical constituents from the polyphenol group (15 flavones, 14 flavonols, 4 flavan-3-ols, 3 flavanones, 1 phenylpropanoid, 2 gallotannins, 1 ellagitannin, 4 phenolic acids, 1 dihydrochalcone, and 3 coumarins) were identified in supercritical extracts of R. acicularis, R. amblyotis, and R. rugosa.

For the first time, a comprehensive analysis of polyphenolic compounds in extracts of Kamchatka honeysuckle berries (Lonicera caerulea var. kamtschatica Sevast.) was carried out. We used 5 samples of wild honeysuckle and one variety sample Elena from the collection of the Kamchatka Research Institute of Agriculture. High-resolution tandem mass spectrometry was used to identify target analytes in the extracts. 58 polyphenolic compounds corresponding to the Caprifoliaceae family have been identified. Among the identified analytes, some compounds were determined for the first time: flavones—hydroxy genistein, diosmetin O-hexoside, chrysoeriol-O-diglucoside; flavanols—herbacetin, myricetin, astragalin, taxifolin-O-hexoside, rhamnocitrin; flavan-3-ols—epiafzelechin, epicatechin-O-hexoside, epigallocatechin-epicatechin dimer; phenolic acids—hydroxyferulic acid, hydroxy methoxy dimethylbenzoic acid, neochlorogenic acid, ellagic acid; stilbenes—pinosylvin, resveratrol and many other polyphenols. The richest in the content of polyphenolic compounds was the wild form variety nos. 5–23 polyphenolic compounds, followed by the Elena variety—21 polyphenolic compounds.

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Relevance. The article presents a comparative metabolomic study of blue honeysuckle (L. caerulea) berries, varieties Volhova, Tomichka, Goluboe vereteno, Amfora, presented in the collection of the Far Eastern Experimental Station of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.Methods. To determine the complex of secondary metabolites in extracts of blue honeysuckle berries. used the method of tandem mass spectrometry. Fractional maceration was used to obtain highly concentrated extracts. In extracts of L. caerulea berries, 75 compounds of the polyphenol group were reliably identified by tandem mass spectrometry.Results. Based on the results of the qualitative identification of the component composition of blue honeysuckle berry extracts, a Venn diagram was constructed showing the similarity and difference in polyphenol groups of various L. caerulea varieties. Berries of L. caerulea contain a large amount of biologically active substances, in particular, they have a large number of compounds with antioxidant activity. The isolated polyphenol complex included compounds of the following groups: flavones, flavonols, flavan-3-ols, flavanones, anthocyanins, stilbenes, coumarins, phenolic acids. Also, for the first time in the extracts of L. caerulea berries, 30 chemical compounds of the polyphenol group were identified (flavones Pentahydroxydimethoxyflavone, Cirsiliol, jaceosidin, Sophoraisoflavone A, Dihydroxy tetramethoxyflavone, Chrysoeriol-O-hexoside, Formononetin-7-O-glucoside, flavon-3-ols (epi)-Afzelechin, (epi)-Catechin gallate, stilbenes Pinosylvin, Resveratrol, Dihydroresveratrol, coumarins Fraxetin, Umbelliferone, etc.), not previously mentioned in studies on L. caerulea.