Real-time and high-precision land cover classification is the foundation for efficient and quantitative research on grassland degradation using remote sensing techniques. In view of the shortcomings of manual surveying and satellite remote sensing, this study focuses on the identification and classification of grass species indicating grassland degradation. We constructed a UAV-based hyperspectral remote sensing system and collected field data in grassland areas. By applying artificial intelligence technology, we developed a 3D_RNet-O model based on convolutional neural networks, effectively addressing technical challenges in hyperspectral remote sensing identification and classification of grassland degradation indicators, such as low reflectance of vegetation, flat spectral curves, and sparse distribution. The results showed that the model achieved a classification accuracy of 99.05% by optimizing hyperparameter combinations based on improving residual block structures. The establishment of the UAV-based hyperspectral remote sensing system and the proposed 3D_RNet-O classification model provide possibilities for further research on low-altitude hyperspectral remote sensing in grassland ecology.