Broccoli sprouts contain health-promoting glucosinolate and phenolic compounds that can be enhanced by applying ultraviolet light (UV). Here, the effect of UVA or UVB radiation on glucosinolate and phenolic profiles was assessed in broccoli sprouts. Sprouts were exposed for 120 min to low intensity and high intensity UVA (UVA_L, UVA_H) or UVB (UVB_L, UVB_H) with UV intensity values of 3.16, 4.05, 2.28 and 3.34 W/m², respectively. Harvest occurred 2 or 24 h post-treatment; and methanol/water or ethanol/water (70%, v/v) extracts were prepared. Seven glucosinolates and 22 phenolics were identified. Ethanol extracts showed higher levels of certain glucosinolates such as glucoraphanin, whereas methanol extracts showed slight higher levels of phenolics. The highest glucosinolate accumulation occurred 24 h after UVB_H treatment, increasing 4-methoxy-glucobrassicin, glucobrassicin and glucoraphanin by ~170, 78 and 73%, respectively. Furthermore, UVA_L radiation and harvest 2 h afterwards accumulated gallic acid hexoside I (~14%), 4-O-caffeoylquinic acid (~42%), gallic acid derivative (~48%) and 1-sinapoyl-2,2-diferulolyl-gentiobiose (~61%). Increases in sinapoyl malate (~12%), gallotannic acid (~48%) and 5-sinapoyl-quinic acid (~121%) were observed with UVB_H Results indicate that UV-irradiated broccoli sprouts could be exploited as a functional food for fresh consumption or as a source of bioactive phytochemicals with potential industrial applications.

The consumption of fruits and vegetables is related to the prevention and treatment of chronic–degenerative diseases due to the presence of secondary metabolites with pharmaceutical activity. Most of these secondary metabolites, also known as nutraceuticals, are present in low concentrations in the plant tissue. Therefore, to improve the health benefits of horticultural crops, it is necessary to increase their nutraceutical content before reaching consumers. Applying ultraviolet radiation (UVR) to fruits and vegetables has been a simple and effective technology to biofortify plant tissue with secondary metabolites. This review article describes the physiological and molecular basis of stress response in plants. Likewise, current literature on the mechanisms and effects of UVA and UVB radiation on the accumulation of different bioactive phytochemicals are reviewed. The literature shows that UVR is an effective tool to biofortify horticultural crops to enhance their nutraceutical content.