Exploring the coupling and coordination relationship between agriculture and tourism industry plays an important role in formulating differentiated integration development policies and promoting regional economic development. We adopt 14 prefectures and cities in Xinjiang, China as examples, and use a coupling coordination model and generalized method of moments estimation to explore the spatial differentiation characteristics and influencing factors between agriculture and tourism industries in various prefectures and cities in Xinjiang from 2009 to 2018. The results indicate: (1) The combined development levels of agriculture and tourism in Xinjiang exhibited a similar development trend over the period, and despite fluctuations in individual years, the overall trend is still increasing. (2) The coupling degree and coordination of agriculture and tourism in various regions presented significant spatial differentiation characteristics. (3) Human capital, residents’ consumption level, and coupling coordination degree are positively correlated, while service capacity, government support, industrial foundation, and market supply and coupling coordination degree are significantly negatively correlated. Finally, based on our results, we present corresponding suggestions on how to improve the level of integrated development of agriculture and tourism in Xinjiang.