Acoustic tweezers with frequency multiplexing enable dynamic manipulation of micro-objects using dual-channel excitation.

Acoustofluidic centrifugation enables the spinning of a fluidic droplet and nanoparticle enrichment and separation.

Acoustic tweezers enable contactless, dynamic, precise, and multifunctional manipulation of bioparticles in Petri dishes.

High-precision isolation of small extracellular vesicles (sEVs) from biofluids is essential toward developing next-generation liquid biopsies and regenerative therapies. However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. Highly precise sEV fractionation without the need for sample preprocessing or complex nanofabrication methods has been demonstrated using ANSWER, showing potential as a powerful tool that will enable more in-depth studies into the complexity, heterogeneity, and functionality of sEV subpopulations.

The limitations of single fluorescent anti‐counterfeiting technologies necessitate the development of more sophisticated encryption methods to protect information and data. Traditional optical anti‐counterfeiting encryption techniques, which rely on light sources with varying wavelengths to identify information, are now insufficient to meet contemporary security demands due to their restricted response to a narrow range of wavelengths. In this study, the fabrication of patterned, lead‐free double perovskite (DP)/poly(vinylidene fluoride‐trifluoroethylene) (P(VDF‐TrFE)) fluorescent piezoelectric composite films (CFs) is reported. These CFs integrate the up‐conversion and down‐conversion photoluminescent properties of Cs ₂ Na _0.8 Ag _0.2 BiCl ₆ :Yb ³⁺ /Er ³⁺ DP crystals with the piezoelectric properties of P(VDF‐TrFE) film, facilitating multi‐modal information protection. The fluorescent signals of different concealed information in CFs are observable under the excitation of 365 nm UV light and 980 nm infrared (IR) light. Additionally, external pressure applied at various locations on the CFs generates corresponding electrical signals, thereby providing triple‐layer encryption for protected information. A multifunctional anti‐counterfeiting device has been further developed by integrating patterned optical and electrical responses onto flexible CFs, achieving synergistic protection of information security in cross fields and bringing a significant advancement to the high‐level anti‐counterfeiting market.