A novel and simple optical biosensor to detect triglycerides (TGs) has been successfully constructed by using pectin hydrogel membrane as the indicator pH and chromoionophore ETH 5294 (CI), with lipase as the catalyst. The enzymatic working system against TGs releasing H⁺ ions will affect the color absorbance of CI. The characterization results show that a TG biosensor has the optimum condition and sensitivity at the phosphate buffer concentration of 50 mM, pH 7, and enzyme loading of 60 μg. The biosensor works at the tripalmitin (TP) concentration range of 100–400 mg/dL. With the sensitivity of 0.001 (∆A/(mg/dL)), the biosensor response reaches stability after five minutes, and the limit of detection (LOD) of the TG optical biosensor is 15 mg/dL. Relative standard deviation (RSD) in a reproducibility test was 2.5%, with a 15-day lifespan.

In a simple and instant procedure for detecting fish freshness, a hydrogel and hydrophilic pectin matrix membrane was used successfully as an optical pH sensor by immobilizing the chromoionophore ETH 5294 (CI), which is very selective and sensitive for the membrane. The Pe/CI optical pH sensor exhibited excellent linearity between pH 5 and pH 9, with a sensor response time of 5 min and reproducibility of 1.49% relative standard deviation (RSD). The sensor showed response stability for 15 days and a response reduction of 8.6%. The sensor’s capability was demonstrated by the detection of fish freshness for 17 days at 4 °C.

PEMBUATAN STICK DARI IKAN TONGKOL (Euthynnus Affinis) UNTUK PEMBERDAYAAN IBU RUMAH TANGGA DI DESA KUTA BLANG, KECAMATAN SAMADUA, KABUPATEN ACEH SELATAN

One of the government's policies in encouraging economic growth from the fishery sector is to increase fish consumption. Arongan Village, Kuala Pesisir Subdistrict, Nagan Raya Regency is one of the villages that has quite large fishery potential. The high potential of fisheries is not always accompanied by a high level of fishery products consumption as well. In this regard, accurate information is needed regarding the level of fish consumption. In this study, the level of fish consumption was divided into three categories, namely low, medium and high consumption levels. This study aimed to determine the level of fish consumption and analyze the pattern of relationships of factors that influence the level of marine fish consumption in the Arongan Village, Kuala Pesisir District, Nagan Raya Regency. The analytical method used was descriptive quantitative method and multinomial logistic regression. The data used in this study was primary data obtained through observation and questionnaires by taking samples based on random sampling. There were 80 respondents. The results showed that there were 36.25% high fish consumption levels, 51.25% moderate fish consumption levels, and 12.5% low fish consumption levels. It was also found that the variables of education, occupation, and income simultaneously did not have a significant effect on the level of fish consumption, while partially, the variables of education, occupation and income did not have a significant effect on the level of marine fish consumption.