A new endemic species, Eranthis tanhoensis sp. nov., is described from the Republic of Buryatia and Irkutsk Province, Russia. It belongs to Eranthis section Shibateranthis and is morphologically similar to E. sibirica and E. stellata. An integrative taxonomic approach, based on cytogenetical, molecular and biochemical analyses, along with morphological data, was used to delimit this new species.

The karyotypes of Adonis amurensis from Amur Oblast,' and Adonis apennina from Altai Republic, Khakassia Republic, and Irkutsk Oblast' have been investigated. The karyotype formula was obtained as 2n = 2x =16 = 8m + 8sm(4sat) for all specimens. We estimated karyotype asymmetry through the calculation of the Coefficient of Variation of Chromosome Length (CVCL), Coefficient of Variation of Centromeric Index (CVCI), and Mean Centromeric Asymmetry (MCA), and determination of Stebbins asymmetry index. The chromosome set of Adonis amurensis was found to be more symmetrical than the chromosome set of Adonis apennina. The average absolute nuclear DNA content (2?–value) was originally determined for Adonis amurensis and Adonis apennina by flow cytometry and attained on average 20.38 pg and 17.29 pg, respectively. Keywords: Adonis L., chromosomes, 2C–value, karyotype, nuclear DNA content, Ranunculaceae References Altinordu F., Peruzzi L., Yu Y., He X. (2016) A tool for the analysis of chromosomes: KaryoType. Taxon, 65(3), 586–592. Cronquist A. (1981) An integrated system of classification of flowering plants. Columbia Univ. Press, New York. Del Grosso F., Pogliani M. (1971) Studio cariologico di Adonis distortus Ten. Biogeographia – The Journal of Integrative Biogeography, 2(1), 69–79. DOI: 10.21426/B62110466 Dole

Comparative karyomorphological analyses of six out of the eight white-flowered species of Eranthis sect. Shibateranthis have been carried out. All studied specimens of E. byunsanensis, E. lobulata, E. pinnatifida, and E. stellata had a somatic chromosome number 2n = 16 with basic chromosome number x = 8. On the contrary, E. tanhoensis and E. sibirica had a basic chromosome number x = 7. The specimens of E. tanhoensis were diploid with 2n = 14, while the specimens of E. sibirica were polyploid with 2n = 42. Monoploid chromosome sets of the investigated diploid species had 4–5 metacentric chromosomes and 2–4 submetacentric/subtelocentric/acrocentric chromosomes. The highest level of interchromosomal asymmetry, estimated via CVCL, was found in E. byunsanensis and E. pinnatifida. The highest levels of intrachromosomal asymmetry (MCA) and heterogeneity in centromere position (CVCI) were found in E. lobulata and E. byunsanensis, while E. sibirica had the most symmetric karyotype. A multivariate PCoA analysis of basic karyotype parameters (2n, x, THL, CVCL, MCA, and CVCI) highlighted no overlap among species accessions, which was also confirmed by LDA. The average absolute monoploid DNA content (1Cx) of the 23 investigated samples of six Eranthis species varied from 9.26 ± 0.25 pg in E. sibirica to 15.93 ± 0.32 pg in E. stellata. Overall karyological affinity was highlighted between E. lobulata and E. stellata, on one side, and between E. byunsanensis and E. pinnatifida, on the other side. Interestingly, there was no significant correlation between total haploid (monoploid) chromosome length (THL) and 1Cx values in these species.

The comparative karyological analysis of Adonis vernalis and Adonis volgensis (Ranunculaceae) from 11 populations from Russia (Altai Territory, Novosibirsk Region, Republic of Khakassia) and Bulgaria was carried out. The somatic chromosome number was 2n = 16 in all studied plants. Chromosome sets of A. vernalis from the Altai Territory (near Sibirskiye Ogni village) and the Republic of Khakassia (Tashtipskiy District), A. volgensis from the Altai Territory (near Chernopyatovo village) and Bulgaria (Kavarna Municipality) consist of eight metacentric and eight submetacentric chromosomes. Two pairs of submetacentric chromosomes had small satellites in short arms. The karyotype formula of these plants was 2n = 2x = 16 = 8m + 4sm + 4smsat. The specimen with the chromosome rearrangement (nonreciprocal translocation) was found in the Altai Territory population. The chromosome mutation has changed the chromosome morphology of submetacentric pair to metacentric pair of different size. The karyotype formula of this specimen was 2n = 2x = 16 = 2n = 10m + 6sm. A similar mutation was previously found out in A. apennina (A. sibirica) from the Tomsk Region. The nuclear DNA content (2C-value) was determined in eight Adonis populations by flow cytometry. The average absolute nuclear DNA content in A. vernalis attained on from 18.88 ± 1.01 to 19.47 ± 1.17 pg, and in A. volgensis attained on from 16.10 ± 0.41 to 16.47 ± 0.47 pg. Differences in 2C-value between A. volgensis and A. vernalis correlated with differences in total chromosome lengths of the set.

The karyotypes (chromosome sets) of Trollius austrosibiricus, T. kytmanovii, and T. riederianus have been investigated for the first time. The karyotype formula has been obtained as 2n = 2x =16 = 2m + 2m/sm + 12sm for Trollius austrosibiricus and T. riederianus, and 2n = 2x =16 = 4m + 12sm for T. kytmanovii. A comparative analysis of the chromosome sets was conducted for these three species and the previously studied Trollius altaicus, T. asiaticus, T. ledebourii, and T. lilacinus (Hegemone lilacina) for several karyological parameters. The average absolute nuclear DNA content (2?-value) was originally determined for Trollius austrosibiricus, T. kytmanovii, T. ledebourii, and T. riederianus by flow cytometry. Keywords: chromosomes, C-value, globe-flower, karyotype, nuclear DNA content, Ranunculaceae, Trollius L. References Altinordu F., Peruzzi L., Yu Y., He X. (2016) A tool for the analysis of chromosomes: KaryoType. Taxon, 65 (3), 586–592. Badaeva E. D., Salina E. A. (2013) Genome structure and chromosomal analysis of plants. Vavilov Journal of Genetics and Breeding, 17 (4/2), 1017–1043 [In Russian]. Baltisberger M., Hörandl E. (2016) Karyotype evolution supports the molecular phylogeny in the genus Ranunculus (Ranunculaceae). Perspectives in Plant Ecology, Evolution and Systematics, 18, 1–14. DOI: 10.1016/j.ppees.2015.11.001 Butkov A. Y. (1953) Hegemone. In: Vvedensky A. I. & Korovin E. P. (eds.), Flora Uzbekistan. 2, 439. “FAN”, Taschkent (In Russian). Czerepanov S. K. (1995) Vascular Plants of Russia and Adjacent States (the Former USSR). Cambridge University Press, Cambridge. Del Grosso F., Pogliani M. (1971) Studio cariologico di Adonis distortus Ten. Biogeographia – The Journal of Integrative Biogeography, 2(1), 69–79. DOI: 10.21426/B62110466 Dole

A comparative karyotype analysis of four species of yellow-flowered Eranthis sect. Eranthis, i.e., E. bulgarica, E. cilicica, E. hyemalis, and E. longistipitata from different areas, has been carried out for the first time. All the studied specimens had somatic chromosome number 2n = 16 with basic chromosome number x = 8. Karyotypes of the investigated plants included five pairs of metacentric chromosomes and three pairs of submetacentric/subtelocentric chromosomes. The chromosome sets of the investigated species differ mainly in the ratio of submetacentric/subtelocentric chromosomes, their relative lengths, and arm ratios. A new oligonucleotide probe was developed and tested to detect 45S rDNA clusters. Using this probe and an oligonucleotide probe to 5S rDNA, 45S and 5S rDNA clusters were localized for the first time on chromosomes of E. cilicica, E. hyemalis, and E. longistipitata. Major 45S rDNA clusters were identified on satellite chromosomes in all the species; in E. cilicica, minor clusters were also identified in the terminal regions of one metacentric chromosome pair. The number and distribution of 5S rDNA clusters is more specific. In E. cilicica, two major clusters were identified in the pericentromeric region of a pair of metacentric chromosomes. Two major clusters in the pericentromeric region of a pair of submetacentric chromosomes and two major clusters in the interstitial region of a pair of metacentric chromosomes were observed in E. longistipitata. E. hyemalis has many clusters of different sizes, localized mainly in the pericentromeric regions. Summarizing new data on the karyotype structure of E. sect. Eranthis and previously obtained data on E. sect. Shibateranthis allowed conclusions to be formed about the clear interspecific karyological differences of the genus Eranthis.