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Prof. Simone Morais
REQUIMTE/LAQV, Instituto Superior de Engenharia do Instituto Politécnico do Porto

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0 Air Pollution
0 Environment, Health, and Safety (EHS)
0 biomonitoring
0 Occupational exposure
0 biosensor development

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biomonitoring
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biosensor development

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Journal article
Published: 18 August 2021 in Foods
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Algae are an underexploited source of natural bioactive compounds in Western countries, so an increasing interest in the valorization of these marine organisms has emerged in recent years. In this work, the effect of extracting solvent on the extraction yield, phenolic content, antioxidant capacity, and antimicrobial activity of nine brown macroalgae species (Ascophyllum nodosum, Himanthalia elongata, Undaria pinnatifida, Pelvetia canaliculata, Saccharina latissima, Bifurcaria bifurcata, Laminaria ochroleuca, Sargassum muticum, and Fucus spiralis) was assessed. Total phenolic content (TPC) and the antioxidant properties of extracts by different assays: radical scavenging activity (DPPH-RSA) and ferric reducing antioxidant power (FRAP) were performed. The antimicrobial activity of extracts was studied against six different foodborne microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Escherichia coli, Salmonella enteritidis, and Pseudomonas aeruginosa. The highest extraction yield was achieved in ethanolic extracts. However, the highest TPC and FRAP values were obtained on the ethyl acetate extracts, especially from A. nodosum. Concerning algal species, the highest TPC and FRAP values were found in A. nodosum, while the highest DPPH-RSA values were achieved in the hexane extracts of B. bifurcata. The antimicrobial activity of algal extracts varied according to the solvent and alga selected, suggesting the species- and solvent-dependent behavior of this property, with B. bifurcata extracts showing the highest results for a wide range of bacteria. Our results provide insight on the characterization of widespread brown algae in the coasts of the North-Western region of the Iberian Peninsula, reflecting multiple health-enhancing properties which may lead to their exploitation in food, pharmacological, and cosmetic industries.

ACS Style

Aurora Silva; Carla Rodrigues; Paula Garcia-Oliveira; Catarina Lourenço-Lopes; Sofia A. Silva; Pascual Garcia-Perez; Ana P. Carvalho; Valentina F. Domingues; M. Fátima Barroso; Cristina Delerue-Matos; Jesus Simal-Gandara; Miguel A. Prieto. Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula. Foods 2021, 10, 1915 .

AMA Style

Aurora Silva, Carla Rodrigues, Paula Garcia-Oliveira, Catarina Lourenço-Lopes, Sofia A. Silva, Pascual Garcia-Perez, Ana P. Carvalho, Valentina F. Domingues, M. Fátima Barroso, Cristina Delerue-Matos, Jesus Simal-Gandara, Miguel A. Prieto. Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula. Foods. 2021; 10 (8):1915.

Chicago/Turabian Style

Aurora Silva; Carla Rodrigues; Paula Garcia-Oliveira; Catarina Lourenço-Lopes; Sofia A. Silva; Pascual Garcia-Perez; Ana P. Carvalho; Valentina F. Domingues; M. Fátima Barroso; Cristina Delerue-Matos; Jesus Simal-Gandara; Miguel A. Prieto. 2021. "Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula." Foods 10, no. 8: 1915.

Journal article
Published: 17 August 2021 in Chemical Engineering Journal
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Biofuel cells are an interesting way of microenergy production that can be advantageously channeled for biosensing applications. In this study, a biofuel cell was developed and applied as a self-powered biosensor for glucose detection. Glucose oxidase was initially tested for the bioanode and, since no evidence of direct electron transfer was observed in the absence of the co-substrate, it was not further considered. The enzymes Pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQGDH) and Bilirubin oxidase (BOx) were immobilized on Pencil graphite electrodes (PGE), previously nanostructured with MWCNT, serving as bioanode and biocathode, respectively. The bifunctional crosslinker 1-pyrenebutanoic acid succinimidyl ester (PBSE) was used as a simple but efficient tethering agent, enabling the establishment of direct electron transfer events. Cyclic and linear sweep voltammetry, and amperometry were employed for individual characterization of bioanode and biocathode as biosensors for the respective enzymatic substrates. A mean sensitivity of 77.7 ± 5.9 μA cm−2 mM−1 was obtained for glucose with a Limit of detection (LOD) of 4.0 ± 2.2 μM, whereas in the detection of O2, the sensitivity and LOD achieved were 336 ± 22 μA cm−2 mM−1 and 3.2 ± 1.0 μM, respectively. Finally, the PQQGDH bioanode and BOx biocathode were conjugated into a biofuel cell and further characterized as a self-powered biosensor for glucose, exhibiting a linear range up to 1 mM, an excellent sensitivity of 8.08 μW cm−2 mM−1, a low LOD of 0.084 mM and long stability (94% of the original response after 12 days). Therefore, PGE transducers and PBSE are valuable strategies for the design of simple and efficient self-powered biosensors.

ACS Style

Álvaro Torrinha; Miguel Tavares; Cristina Delerue-Matos; Simone Morais. A self-powered biosensor for glucose detection using modified pencil graphite electrodes as transducers. Chemical Engineering Journal 2021, 426, 131835 .

AMA Style

Álvaro Torrinha, Miguel Tavares, Cristina Delerue-Matos, Simone Morais. A self-powered biosensor for glucose detection using modified pencil graphite electrodes as transducers. Chemical Engineering Journal. 2021; 426 ():131835.

Chicago/Turabian Style

Álvaro Torrinha; Miguel Tavares; Cristina Delerue-Matos; Simone Morais. 2021. "A self-powered biosensor for glucose detection using modified pencil graphite electrodes as transducers." Chemical Engineering Journal 426, no. : 131835.

Journal article
Published: 05 July 2021 in Food and Chemical Toxicology
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Considering the importance of seaweeds for the development of sustainable and innovative food products, this study aimed to characterize the impact of hydrothermal processing on iodine, sodium, potassium, selenium, and arsenic concentrations of four seaweed species (S. latissima, L. digitata, U. pinnatifida, and C. crispus) and on the associated health risks-benefits for consumers. These elements revealed a common pattern for leachable fractions of iodine, total arsenic, and selenium: L. digitata ≥ S. latissima > C. crispus > U. pinnatifida after rehydration and boiling during different periods. The behavior for sodium was: S. latissima > L. digitata > C. crispus > U. pinnatifida, and for potassium: U. pinnatifida > L. digitata > S. latissima > C. crispus. Generally, the species that attained more significant losses were S. latissima and L. digitata. A health-relevant sodium/potassium ratio below 0.7 was found for all species except for U. pinnatifida. In some species, the risk-benefit analysis revealed that high iodine and arsenic levels might promote risks for consumption, even after 20 min boiling, but 5 g of processed U. pinnatifida could contribute to adequate iodine, sodium, potassium, and selenium intakes for all population groups. Standardized processing treatments of seaweeds can open new opportunities for the sector.

ACS Style

Helena Correia; Cristina Soares; Simone Morais; Edgar Pinto; António Marques; Maria Leonor Nunes; Agostinho Almeida; Cristina Delerue-Matos. Seaweeds rehydration and boiling: Impact on iodine, sodium, potassium, selenium, and total arsenic contents and health benefits for consumption. Food and Chemical Toxicology 2021, 155, 112385 .

AMA Style

Helena Correia, Cristina Soares, Simone Morais, Edgar Pinto, António Marques, Maria Leonor Nunes, Agostinho Almeida, Cristina Delerue-Matos. Seaweeds rehydration and boiling: Impact on iodine, sodium, potassium, selenium, and total arsenic contents and health benefits for consumption. Food and Chemical Toxicology. 2021; 155 ():112385.

Chicago/Turabian Style

Helena Correia; Cristina Soares; Simone Morais; Edgar Pinto; António Marques; Maria Leonor Nunes; Agostinho Almeida; Cristina Delerue-Matos. 2021. "Seaweeds rehydration and boiling: Impact on iodine, sodium, potassium, selenium, and total arsenic contents and health benefits for consumption." Food and Chemical Toxicology 155, no. : 112385.

Review article
Published: 20 June 2021 in Environment International
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Firefighting is physically and physiologically exhausting besides encompassing exposure to toxic fire emissions. Biomonitoring studies from the past five years have been significantly contributing to characterize the occupational-related health effects in this group of professionals and to improve risk assessment. Therefore, this study gathers and critically discusses the most characterized biomarkers of effect (oxidative stress, DNA and protein damage, stress hormones, inflammation, and vascular, lung, and liver injury), including those potentially more promising to be explored in future studies, and their relation with health outcomes. Various studies proved an association between exposures to fire emissions and/or heat and significantly altered values of biomarkers of inflammation (soluble adhesion molecules, tumor necrosis factor, interleukins, and leucocyte count), vascular damage and tissue injury (pentraxin-3, vascular endothelial growth factor, and cardiac troponin T) in firefighting forces. Moreover, preliminary data of DNA damage in blood, urinary mutagenicity and 8-isoprostaglandin in exhaled breath condensate suggest that these biomarkers of oxidative stress should be further explored. However, most of the reported studies are based on cross-sectional designs, which limit full identification and characterization of the risk factors and their association with development of work-related diseases. Broader studies based on longitudinal designs and strongly supported by the analysis of several types of biomarkers in different biological fluids are further required to gain deeper insights into the firefighters occupational related health hazards and contribute to implementation of new or improved surveillance programs.

ACS Style

Bela Barros; Marta Oliveira; Simone Morais. Firefighters’ occupational exposure: Contribution from biomarkers of effect to assess health risks. Environment International 2021, 156, 106704 .

AMA Style

Bela Barros, Marta Oliveira, Simone Morais. Firefighters’ occupational exposure: Contribution from biomarkers of effect to assess health risks. Environment International. 2021; 156 ():106704.

Chicago/Turabian Style

Bela Barros; Marta Oliveira; Simone Morais. 2021. "Firefighters’ occupational exposure: Contribution from biomarkers of effect to assess health risks." Environment International 156, no. : 106704.

Journal article
Published: 12 June 2021 in Foods
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The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.

ACS Style

Cristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods 2021, 10, 1366 .

AMA Style

Cristina Soares, Sara Sousa, Susana Machado, Elsa Vieira, Ana Carvalho, Maria Ramalhosa, Simone Morais, Manuela Correia, Teresa Oliva-Teles, Valentina Domingues, Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods. 2021; 10 (6):1366.

Chicago/Turabian Style

Cristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. 2021. "Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination." Foods 10, no. 6: 1366.

Journal article
Published: 07 June 2021 in Separations
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A multiresidue analytical methodology based on a solid-phase microextraction (SPME), followed by gas chromatography-ion trap mass spectrometry (GC–IT/MS), has been developed for trace analysis of 20 known or suspected endocrine-disrupting pesticides. The SPME conditions are optimized considering several key parameters to obtain the maximum sensitivity. After the optimization, the method validation is performed, and the limits of detection (ranged from 2–150 ng/L) and the coefficient of determination (above 0.990) of studied compounds are determined for all the analytes. A robust sampling of twenty sampling points of surface water samples from the north and center of Portugal is performed, and the validated methodology is applied. In total, 20 compounds from four chemical families (13 organochlorine, 1 organophosphorus, 2 dicarboximide, and 4 pyrethroids) are studied, and the pesticides most frequently detected are eight organochlorine pesticides (α-, β-HCHs, lindane, HCB, o,p′-DDT, p,p′-DDE, p,p′-DDD, α-endosulfan), cypermethrin, and vinclozolin.

ACS Style

José Vera; Virgínia Fernandes; Luísa Correia-Sá; Catarina Mansilha; Cristina Delerue-Matos; Valentina Domingues. Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS. Separations 2021, 8, 81 .

AMA Style

José Vera, Virgínia Fernandes, Luísa Correia-Sá, Catarina Mansilha, Cristina Delerue-Matos, Valentina Domingues. Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS. Separations. 2021; 8 (6):81.

Chicago/Turabian Style

José Vera; Virgínia Fernandes; Luísa Correia-Sá; Catarina Mansilha; Cristina Delerue-Matos; Valentina Domingues. 2021. "Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS." Separations 8, no. 6: 81.

Original paper
Published: 24 May 2021 in Waste and Biomass Valorization
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Recently, the value of wood residues has received increasing attention due to the presence of pharmacologically active compounds. In the present study, apple bark was treated with subcritical water (SWE) with the aim of bioactivity determination and chemical analysis by GC–MS applying two different sample preparation protocols and by HPLC. Extracts were screened for their total antioxidant capacity by means of total phenolic (TPC) and flavonoid contents (TFC), ferric reducing antioxidant power (FRAP) and DPPH-radical scavenging activity (DPPH-RSA). Anti-microbial and anti-proliferative activities of the apple bark subcritical water extracts were also examined. Different lignin and cellulose degradation products were identified by GC–MS, as well as naturally present compounds. Identified compounds encompassed organic and fatty acids, alcohols, sugars, phenol, benzaldehyde, cinnamic acid, cinnamaldehyde and azulene derivatives, as well as other organic compounds. Phenolic profiles of apple bark extracts defined by HPLC–PDA analysis revealed that the main contributors were gallic acid (9.4 ± 0.5 mg/g DW) and catechin (8.3 ± 0.4 mg/g DW). The diversity of the extracts composition supports the fact that this medium is able to extract compounds of different polarities, simultaneously acting as catalyst and reactant. The characterized apple bark extracts demonstrated good antioxidant (TPC = 31.47 ± 1.86 mg GAE/g; TFC = 17.40 ± 1.89 mg EE/g; FRAP = 22.45 ± 2.22 mg AAE/g; DPPH-RSA = 22.57 ± 2.24 mg Trolox E/g DW), antimicrobial (MIC = 19.53–156.25 μg/mL) and anti-proliferative activity (IC50 = 19.88–47.44 μg/mL). The findings of this study showed that SWE can be used as an environmentally friendly and safe technique for the valorization of apple tree wood residues, applicable in food and pharmaceutical industries.

ACS Style

Jaroslava Švarc-Gajić; Víctor Cerdà; Cristina Delerue-Matos; Pavle Mašković; Sabrina Clavijo; Ruth Suarez; Aleksandra Cvetanović; Maria João Ramalhosa; M. Fátima Barroso; Manuela Moreira; Simone Morais; Hannes Withouck; Annick Boeykens. Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water. Waste and Biomass Valorization 2021, 1 -14.

AMA Style

Jaroslava Švarc-Gajić, Víctor Cerdà, Cristina Delerue-Matos, Pavle Mašković, Sabrina Clavijo, Ruth Suarez, Aleksandra Cvetanović, Maria João Ramalhosa, M. Fátima Barroso, Manuela Moreira, Simone Morais, Hannes Withouck, Annick Boeykens. Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water. Waste and Biomass Valorization. 2021; ():1-14.

Chicago/Turabian Style

Jaroslava Švarc-Gajić; Víctor Cerdà; Cristina Delerue-Matos; Pavle Mašković; Sabrina Clavijo; Ruth Suarez; Aleksandra Cvetanović; Maria João Ramalhosa; M. Fátima Barroso; Manuela Moreira; Simone Morais; Hannes Withouck; Annick Boeykens. 2021. "Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water." Waste and Biomass Valorization , no. : 1-14.

Review
Published: 21 May 2021 in International Journal of Environmental Research and Public Health
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Copper chrome arsenate (CCA) water-borne solution used to be widely used to make timber highly resistant to pests and fungi, in particular, wood products designed for outdoor use. Nowadays, CCA is a restricted chemical product in most countries, since potential environmental and health risks were reported due to dermal contact with CCA residues from treated structures and the surrounding soil, as well as the contamination of soils. However, large quantities of CCA-treated timber are still in use in framings, outdoor playground equipment, landscaping, building poles, jetty piles, and fencing structures around the world, thus CCA remains a source of pollutants to the environment and of increasing toxic metal/metalloid exposure (mainly in children). International efforts have been dedicated to the treatment of materials impregnated with CCA, however not only does some reuse of CCA-treated timber still occur, but also existing structures are leaking the toxic compounds into the environment, with impacts on the environment and animal and human health. This study highlights CCA mechanisms and the documented consequences in vivo of its exposure, as well as the adverse environmental and health impacts.

ACS Style

Simone Morais; Henrique Fonseca; Sónia Oliveira; Helena Oliveira; Vivek Gupta; Bechan Sharma; Maria De Lourdes Pereira. Environmental and Health Hazards of Chromated Copper Arsenate-Treated Wood: A Review. International Journal of Environmental Research and Public Health 2021, 18, 5518 .

AMA Style

Simone Morais, Henrique Fonseca, Sónia Oliveira, Helena Oliveira, Vivek Gupta, Bechan Sharma, Maria De Lourdes Pereira. Environmental and Health Hazards of Chromated Copper Arsenate-Treated Wood: A Review. International Journal of Environmental Research and Public Health. 2021; 18 (11):5518.

Chicago/Turabian Style

Simone Morais; Henrique Fonseca; Sónia Oliveira; Helena Oliveira; Vivek Gupta; Bechan Sharma; Maria De Lourdes Pereira. 2021. "Environmental and Health Hazards of Chromated Copper Arsenate-Treated Wood: A Review." International Journal of Environmental Research and Public Health 18, no. 11: 5518.

Review article
Published: 03 May 2021 in TrAC Trends in Analytical Chemistry
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Carbon cloth and carbon paper have unique characteristics such as conductivity, mechanical strength, porosity, reduced dimensions, and adjustable size and shape. With these materials, different simple modification strategies can be employed, with emphasis on the direct growth of metal-based catalysts through single-step hydrothermal methods. Thus, owing to these interesting features, highly efficient, versatile, and disposable electrochemical (bio)sensors based on carbon cloth and carbon paper have been designed for biomolecules, biomarkers, and hazardous and chemical compounds detection in clinical, environmental and food samples, as well as in industrial products. Moreover, the advantage of facile integration in miniaturized and portable devices, envisages their successful application in point-of-care diagnostics and in-situ measurements. Therefore, this review highlights the main advances and the current research gaps concerning the design of these novel electroanalytical tools.

ACS Style

Álvaro Torrinha; Simone Morais. Electrochemical (bio)sensors based on carbon cloth and carbon paper: An overview. TrAC Trends in Analytical Chemistry 2021, 142, 116324 .

AMA Style

Álvaro Torrinha, Simone Morais. Electrochemical (bio)sensors based on carbon cloth and carbon paper: An overview. TrAC Trends in Analytical Chemistry. 2021; 142 ():116324.

Chicago/Turabian Style

Álvaro Torrinha; Simone Morais. 2021. "Electrochemical (bio)sensors based on carbon cloth and carbon paper: An overview." TrAC Trends in Analytical Chemistry 142, no. : 116324.

Paper
Published: 08 April 2021 in Analytical Methods
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A simple and robust sensor (fMWCNT–Nafion®0.5%/GCE) for determination of imidacloprid, a widely used neonicotinoid, was developed using a glassy carbon electrode (GCE) modified with functionalized multi-walled carbon nanotubes (fMWCNT) and Nafion®.

ACS Style

Evellin E. S. Bruzaca; Raissa C. de Oliveira; Mateus S. S. Duarte; Camila Pinheiro Sousa; Simone Morais; Adriana Nunes Correia; Pedro de Lima-Neto. Electrochemical sensor based on multi-walled carbon nanotubes for imidacloprid determination. Analytical Methods 2021, 13, 2124 -2136.

AMA Style

Evellin E. S. Bruzaca, Raissa C. de Oliveira, Mateus S. S. Duarte, Camila Pinheiro Sousa, Simone Morais, Adriana Nunes Correia, Pedro de Lima-Neto. Electrochemical sensor based on multi-walled carbon nanotubes for imidacloprid determination. Analytical Methods. 2021; 13 (18):2124-2136.

Chicago/Turabian Style

Evellin E. S. Bruzaca; Raissa C. de Oliveira; Mateus S. S. Duarte; Camila Pinheiro Sousa; Simone Morais; Adriana Nunes Correia; Pedro de Lima-Neto. 2021. "Electrochemical sensor based on multi-walled carbon nanotubes for imidacloprid determination." Analytical Methods 13, no. 18: 2124-2136.

Book chapter
Published: 18 March 2021 in Advances in Applied Microbiology
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Firefighters are the professional force at high risk of suffering potential health consequences due to their chronic exposure to numerous hazardous pollutants during firefighting activities. Unfortunately, determination of fire emission exposure is very challenging. As such, the identification and development of appropriate biomarkers is critical in meeting this need. This chapter presents a critical review of current information related with the use of different urinary biomarkers of effect and exposure in occupationally exposed firefighters over the last 25 years. Evidence suggests that urinary isoprostanes and mutagenicity testing are promising biomarkers of early oxidative stress. Data indicate that firefighters participating in firefighting activities present with increased urinary biomarkers of exposure. These include polycyclic aromatic hydrocarbons, heavy metals and metalloids, organo-chlorine and -phosphorus compounds, environmental phenols, phthalates, benzene and toluene. More studies are urgently needed to better evaluate firefighter occupational safety and health and to support the implementation of preventive measures and mitigation strategies to promote the protection of this chronically exposed group of workers.

ACS Style

Bela Barros; Marta Oliveira; Simone Morais. Urinary biohazard markers in firefighters. Advances in Applied Microbiology 2021, 1 .

AMA Style

Bela Barros, Marta Oliveira, Simone Morais. Urinary biohazard markers in firefighters. Advances in Applied Microbiology. 2021; ():1.

Chicago/Turabian Style

Bela Barros; Marta Oliveira; Simone Morais. 2021. "Urinary biohazard markers in firefighters." Advances in Applied Microbiology , no. : 1.

Journal article
Published: 15 January 2021 in Talanta
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In the present work, the exceptional electrochemical properties of carbon paper were characterized and explored in the development of a sensor for the anti-inflammatory drug, ketoprofen. An initial electrochemical characterization of the carbon paper with a redox indicator revealed much higher voltammetric peaks in comparison with other carbon-based electrodes, namely, screen-printed carbon electrode, boron-doped diamond electrode, glassy carbon electrode, pyrolytic graphite electrode, and pencil graphite electrode, predicting a good sensing performance. Ketoprofen showed a pronounced cathodic peak around −1.1 V vs Ag/AgCl (KCl, 3 M) at pH 5 in Britton-Robinson buffer, presenting a diffusion-controlled process. An optimized differential pulse voltammetry procedure was employed for ketoprofen determination achieving a limit of detection of 0.11 ± 0.01 μM, a linearity up to 6.02 μM, and a high sensitivity of 24 ± 4 or 35 ± 3 μA μM−1 cm−2 (depending on the calibration range: 0.088–1.96 μM or 1.96–6.02 μM, respectively) with acceptable (6–15% relative standard deviation) reproducibility and repeatability considering the challenging conditions of its detection in aqueous solutions. The sensor revealed to be highly selective in the presence of common interferents and other widely consumed anti-inflammatory drugs. Moreover, the developed sensor reached good accuracy in wastewater and fish samples with recoveries varying from 82.3 ± 4.4 to 88.6 ± 4.5%.

ACS Style

Álvaro Torrinha; Marco Martins; Miguel Tavares; Cristina Delerue-Matos; Simone Morais. Carbon paper as a promising sensing material: Characterization and electroanalysis of ketoprofen in wastewater and fish. Talanta 2021, 226, 122111 .

AMA Style

Álvaro Torrinha, Marco Martins, Miguel Tavares, Cristina Delerue-Matos, Simone Morais. Carbon paper as a promising sensing material: Characterization and electroanalysis of ketoprofen in wastewater and fish. Talanta. 2021; 226 ():122111.

Chicago/Turabian Style

Álvaro Torrinha; Marco Martins; Miguel Tavares; Cristina Delerue-Matos; Simone Morais. 2021. "Carbon paper as a promising sensing material: Characterization and electroanalysis of ketoprofen in wastewater and fish." Talanta 226, no. : 122111.

Journal article
Published: 30 December 2020 in International Journal of Environmental Research and Public Health
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Grilling activities release large amounts of hazardous pollutants, but information on restaurant grill workers’ exposure to polycyclic aromatic hydrocarbons (PAHs) is almost inexistent. This study assessed the impact of grilling emissions on total workers’ exposure to PAHs by evaluating the concentrations of six urinary biomarkers of exposure (OHPAHs): naphthalene, acenaphthene, fluorene, phenanthrene, pyrene, and benzo(a)pyrene. Individual levels and excretion profiles of urinary OHPAHs were determined during working and nonworking periods. Urinary OHPAHs were quantified by high-performance liquid-chromatography with fluorescence detection. Levels of total OHPAHs (∑OHPAHs) were significantly increased (about nine times; p ≤ 0.001) during working comparatively with nonworking days. Urinary 1-hydroxynaphthalene + 1-hydroxyacenapthene and 2-hydroxyfluorene presented the highest increments (ca. 23- and 6-fold increase, respectively), followed by 1-hydroxyphenanthrene (ca. 2.3 times) and 1-hydroxypyrene (ca. 1.8 times). Additionally, 1-hydroxypyrene levels were higher than the benchmark, 0.5 µmol/mol creatinine, in 5% of exposed workers. Moreover, 3-hydroxybenzo(a)pyrene, biomarker of exposure to carcinogenic PAHs, was detected in 13% of exposed workers. Individual excretion profiles showed a cumulative increase in ∑OHPAHs during consecutive working days. A principal component analysis model partially discriminated workers’ exposure during working and nonworking periods showing the impact of grilling activities. Urinary OHPAHs were increased in grill workers during working days.

ACS Style

Marta Oliveira; Sílvia Capelas; Cristina Delerue-Matos; Simone Morais. Grill Workers Exposure to Polycyclic Aromatic Hydrocarbons: Levels and Excretion Profiles of the Urinary Biomarkers. International Journal of Environmental Research and Public Health 2020, 18, 230 .

AMA Style

Marta Oliveira, Sílvia Capelas, Cristina Delerue-Matos, Simone Morais. Grill Workers Exposure to Polycyclic Aromatic Hydrocarbons: Levels and Excretion Profiles of the Urinary Biomarkers. International Journal of Environmental Research and Public Health. 2020; 18 (1):230.

Chicago/Turabian Style

Marta Oliveira; Sílvia Capelas; Cristina Delerue-Matos; Simone Morais. 2020. "Grill Workers Exposure to Polycyclic Aromatic Hydrocarbons: Levels and Excretion Profiles of the Urinary Biomarkers." International Journal of Environmental Research and Public Health 18, no. 1: 230.

Journal article
Published: 09 December 2020 in Applied Sciences
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The oil industry generates great quantities of oilseed cakes that remain after oil extraction. New technologies are required for their valorization, owing to their high nutritional value. Pumpkin, flax and hemp seed cakes were extracted by subcritical water under different conditions that included different gas atmospheres and homogenous catalysis, and for the first time their properties were directly compared. Extracts obtained in a nitrogen atmosphere, nitrogen atmosphere with the addition of a catalyst, and carbon dioxide atmosphere were chemically and nutritionally characterised. In the aqueous extracts obtained under different extraction conditions, the content of lipids, proteins and selected minerals (calcium, potassium, sodium and phosphorus) were determined. A detailed amino acid profile was determined by chromatographic analysis. The highest relative content of essential amino acids was observed in pumpkin seed extracts (51.49 ± 0.47 to 58.58 ± 0.45 mg/100 g dry extract), whereas hemp seed extracts were the richest in flavour amino acids aspartic acid, glutamic acid and alanine. Extraction in a carbon dioxide atmosphere or in nitrogen atmosphere with a HCl modifier released generally more minerals into the aqueous phase. Aqueous oilseed cake extracts demonstrated a favorable chemical composition and great nutritional value, opening new possibilities for exploitation of this biowaste. Based on the obtained results, oilseed cake extracts obtained by subcritical water have great potential to be used for the fortification of different food products, as well as in cosmetics.

ACS Style

Jaroslava Švarc-Gajić; Simone Morais; Cristina Delerue-Matos; Elsa F. Vieira; Giorgia Spigno. Valorization Potential of Oilseed Cakes by Subcritical Water Extraction. Applied Sciences 2020, 10, 8815 .

AMA Style

Jaroslava Švarc-Gajić, Simone Morais, Cristina Delerue-Matos, Elsa F. Vieira, Giorgia Spigno. Valorization Potential of Oilseed Cakes by Subcritical Water Extraction. Applied Sciences. 2020; 10 (24):8815.

Chicago/Turabian Style

Jaroslava Švarc-Gajić; Simone Morais; Cristina Delerue-Matos; Elsa F. Vieira; Giorgia Spigno. 2020. "Valorization Potential of Oilseed Cakes by Subcritical Water Extraction." Applied Sciences 10, no. 24: 8815.

Journal article
Published: 01 December 2020 in Food and Chemical Toxicology
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This work assessed the concentrations of 18 polycyclic aromatic hydrocarbons (PAHs) in edible tissues of whitemouth croaker (Micropogonias furnieri) and meagre (Argyrosomus regius) captured in different Atlantic Ocean fishing areas and available to Brazilian and Portuguese consumers. Total PAH (∑PAHs) levels ranged from 1.32 to 5.41 μg/kg ww in wild-caught croaker and 2.66 (wild)-18.0 (farmed) μg/kg ww in meagre. Compounds with 2- and 3-rings represented 54-86% of ∑PAHs in the muscle tissues of wild-caught species (croaker and meagre) being naphthalene, fluorene, and phenanthrene the predominant compounds. ∑PAHs in farmed meagre were 4-7 times higher than in wild-caught meagre, with 2-, 3-, 4-, 5-, and 6-ring compounds representing 15, 18, 44, 22, and 1% of ∑PAHs, respectively. Benzo(a)pyrene levels in farmed meagre varied between 0.06 and 0.34 μg/kg ww. Crude oil refining and combustion sources were identified as the major sources of PAHs in FAO 27, 34 and 41 Atlantic Ocean fishing areas. The biometric characteristics (length, weight, moisture, and fat content) and ∑PAHs allowed to discriminate between wild-caught and farmed meagre samples and between meagre and croaker. Assessment of carcinogenic risks suggested that a diet exclusive on farmed meagre may pose additional risks for the health of European top consumers fish.

ACS Style

Marta Oliveira; Carolina De Gasperi Portella; Maria João Ramalhosa; Cristina Delerue-Matos; L.S. Sant’Ana; Simone Morais. Polycyclic aromatic hydrocarbons in wild and farmed whitemouth croaker and meagre from different Atlantic Ocean fishing areas: Concentrations and human health risk assessment. Food and Chemical Toxicology 2020, 146, 111797 .

AMA Style

Marta Oliveira, Carolina De Gasperi Portella, Maria João Ramalhosa, Cristina Delerue-Matos, L.S. Sant’Ana, Simone Morais. Polycyclic aromatic hydrocarbons in wild and farmed whitemouth croaker and meagre from different Atlantic Ocean fishing areas: Concentrations and human health risk assessment. Food and Chemical Toxicology. 2020; 146 ():111797.

Chicago/Turabian Style

Marta Oliveira; Carolina De Gasperi Portella; Maria João Ramalhosa; Cristina Delerue-Matos; L.S. Sant’Ana; Simone Morais. 2020. "Polycyclic aromatic hydrocarbons in wild and farmed whitemouth croaker and meagre from different Atlantic Ocean fishing areas: Concentrations and human health risk assessment." Food and Chemical Toxicology 146, no. : 111797.

Chapter
Published: 01 December 2020 in Sustainable Agriculture Reviews
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The importance of food quality has become a crucial issue due to the widespread use of pesticides in agriculture. The frequent use of pesticides has resulted in contamination of the environmental, fruit crops and also caused potential risk to human health. Strawberries are at the top of the list of fruits with the highest concentration of pesticides. To date, several analytical techniques have been used for the extraction and detection of pesticides in strawberries, from classic to advanced analysis methodologies. In this chapter, the studies published by the scientific community performed from 2000 to the present, concerning the extraction and analysis of pesticide residues in strawberries, were discussed. The most broadly used detection technique for the analyse of pesticides in strawberries is mass spectrometry combined with gas and/or liquid chromatography. In general, multi-residue methods with selective sample treatment methodologies have been developed for this purpose. Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS), liquid-liquid, solid-liquid, and solid-phase extraction and microextractions are the selected sample preparation techniques. The analytical parameters of the methodology and the findings on pesticide residues are described. The presented chapter shows the importance of these data in terms of food safety on the situation regarding pesticide residue in strawberries and human health regarding the risk of the ingestion of pesticides contaminated strawberries.

ACS Style

Virgínia Cruz Fernandes; M. Luz Maia; Luísa Correia Sá; Sara Sousa; Paula Paíga; José L. Vera; Valentina F. Domingues; Cristina Delerue-Matos. Extraction Procedures and Chromatography of Pesticides Residues in Strawberries. Sustainable Agriculture Reviews 2020, 167 -201.

AMA Style

Virgínia Cruz Fernandes, M. Luz Maia, Luísa Correia Sá, Sara Sousa, Paula Paíga, José L. Vera, Valentina F. Domingues, Cristina Delerue-Matos. Extraction Procedures and Chromatography of Pesticides Residues in Strawberries. Sustainable Agriculture Reviews. 2020; ():167-201.

Chicago/Turabian Style

Virgínia Cruz Fernandes; M. Luz Maia; Luísa Correia Sá; Sara Sousa; Paula Paíga; José L. Vera; Valentina F. Domingues; Cristina Delerue-Matos. 2020. "Extraction Procedures and Chromatography of Pesticides Residues in Strawberries." Sustainable Agriculture Reviews , no. : 167-201.

Chapter
Published: 01 December 2020 in Sustainable Agriculture Reviews
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Pyrethroids are a class of extensively used insecticides for human and animal health protection. Concern has been growing about the possible human health effects from environmental exposure, e.g. chronic exposure to low levels, to pyrethroids. Several metabolites, specific or common to different pyrethroids, can be used for the assessment of pyrethroids exposure. 3-Phenoxybenzoic acid is a major urinary biomarker for numerous pyrethroids used nowadays and has been extensively analyzed and reported. Biomonitoring pyrethroids in human samples is undoubtedly necessary in order to support the clarification of potential correlations between exposure and adverse health effects. The present chapter is an overview of pyrethroids’ metabolitesanalyses in human urine samples, including analytical methods and reported values. Data from several studies from 1990 until March 2019 were collected. Studies from different countries and continents that assess exposure in diverse populations are addressed and different applied analytical methodologies are also discussed. Additionally, human biomonitoring was performed based on highly sensitive analytical methods and reports values for several metabolites, showing the relevance of human biomonitoring of pyrethroids. Some metabolites were below the human biomonitoring assessment values of 2 μg/L for the general population. However, we must consider that exceeds in the upper exposure percentiles of the general population, e.g. children.

ACS Style

Luísa Correia Sá; Sara Sousa; Virgínia Cruz Fernandes; M. Luz Maia; Cristina Delerue-Matos; Valentina F. Domingues. Pyrethroids Metabolites in Human Urine Samples. Sustainable Agriculture Reviews 2020, 227 -270.

AMA Style

Luísa Correia Sá, Sara Sousa, Virgínia Cruz Fernandes, M. Luz Maia, Cristina Delerue-Matos, Valentina F. Domingues. Pyrethroids Metabolites in Human Urine Samples. Sustainable Agriculture Reviews. 2020; ():227-270.

Chicago/Turabian Style

Luísa Correia Sá; Sara Sousa; Virgínia Cruz Fernandes; M. Luz Maia; Cristina Delerue-Matos; Valentina F. Domingues. 2020. "Pyrethroids Metabolites in Human Urine Samples." Sustainable Agriculture Reviews , no. : 227-270.

Review
Published: 17 October 2020 in Antibiotics
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The search for food resources is a constant in human history. Nowadays, the search for natural and safe food supplies is of foremost importance. Accordingly, there is a renewed interest in eco-friendly and natural products for substitution of synthetic additives. In addition, microbial contamination of food products during their obtaining and distribution processes is still a sanitary issue, and an important target for the food industry is to avoid food contamination and its related foodborne illnesses. These diseases are fundamentally caused by certain microorganisms listed in this review and classified according to their Gram negative or positive character. Algae have proven to possess high nutritional value and a wide variety of biological properties due to their content in active compounds. Among these capabilities, macroalgae are recognized for having antimicrobial properties. Thus, the present paper revises the actual knowledge of microbial contaminants in the food industry and proposes antimicrobial algal compounds against those pathogenic bacteria responsible for food contamination as valuable molecules for its growth inhibition. The capacity of algae extracts to inhibit some major food pathogen growth was assessed. Moreover, the main applications of these compounds in the food industry were discussed while considering their favorable effects in terms of food safety and quality control.

ACS Style

Aurora Silva; Sofia A. Silva; C. Lourenço-Lopes; C. Jimenez-Lopez; M. Carpena; P. Gullón; M. Fraga-Corral; V. F. Domingues; M. Fátima Barroso; J. Simal-Gandara; M. A. Prieto. Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens. Antibiotics 2020, 9, 712 .

AMA Style

Aurora Silva, Sofia A. Silva, C. Lourenço-Lopes, C. Jimenez-Lopez, M. Carpena, P. Gullón, M. Fraga-Corral, V. F. Domingues, M. Fátima Barroso, J. Simal-Gandara, M. A. Prieto. Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens. Antibiotics. 2020; 9 (10):712.

Chicago/Turabian Style

Aurora Silva; Sofia A. Silva; C. Lourenço-Lopes; C. Jimenez-Lopez; M. Carpena; P. Gullón; M. Fraga-Corral; V. F. Domingues; M. Fátima Barroso; J. Simal-Gandara; M. A. Prieto. 2020. "Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens." Antibiotics 9, no. 10: 712.

Journal article
Published: 09 September 2020 in Journal of Pharmaceutical and Biomedical Analysis
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Synthetic musks and organophosphorus pesticides represent a potential risk to the human health since exposure can lead to distinct types of carcinogenesis and endocrine disorders. These are lipophilic compounds as such, prone to deposit and persist in fat tissues, mainly in adipose tissue. Very few studies have reported on the occurrence and accumulation profile of these contaminants in human adipose tissue. Analytical methods for the detection and quantification of synthetic musks and organophosphorus pesticides in adipose tissue are lacking. In this study, the efficacy of different extraction with ultrasonic homogenizer and dispersive solid-phase extraction (d-SPE) clean-up methods were evaluated in human adipose tissue. The relative sample clean-up was assessed by measurement of total lipid content. The quantification of four synthetic musks and six organophosphorus pesticides were performed by gas chromatography (GC) mass spectrometry (MS) and flame photometric detection (FPD), respectively. The d-SPE clean-up with 50 mg PSA, 150 mg MgSO4, 100 mg C18EC and 50 mg Z-Sep provided the most effective clean-up, removing the greatest amount of interfering substances including lipids and simultaneously ensuring good chromatographic separation and recoveries. Method detection limits were between 4 to 9 ng/g for synthetic musk and 1 to 7 ng/g for organophosphorus pesticides in adipose tissue. The proposed method was applied to adipose tissue of obese patients and positive samples were confirmed with GC tandem mass spectrometry. Galaxolide was found in all the samples tested with concentrations ranging from 0.08 to 0.5 μg/g of adipose tissue. No other synthetic musk studied was detected. Organophosphorus pesticides were not found in the analysed samples. The developed analytical procedures were successful and can easily be applied to biomonitoring these compounds in human adipose tissue.

ACS Style

Sara Sousa; Diogo Pestana; Gil Faria; Fernando Vasconcelos; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue. Journal of Pharmaceutical and Biomedical Analysis 2020, 191, 113598 .

AMA Style

Sara Sousa, Diogo Pestana, Gil Faria, Fernando Vasconcelos, Cristina Delerue-Matos, Conceição Calhau, Valentina Fernandes Domingues. Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue. Journal of Pharmaceutical and Biomedical Analysis. 2020; 191 ():113598.

Chicago/Turabian Style

Sara Sousa; Diogo Pestana; Gil Faria; Fernando Vasconcelos; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. 2020. "Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue." Journal of Pharmaceutical and Biomedical Analysis 191, no. : 113598.

Review article
Published: 08 September 2020 in Heliyon
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Shrimps are widely distributed in coastal areas, estuaries and rivers. Although this shellfish is a good source of nutrients, it can also accumulate environmental contaminants, such as organochlorine pesticides (OCPs), brominated flame retardants (BFRs), synthetic musks (SMs) and polycyclic aromatic hydrocarbons (PAHs). Due to their bioaccumulative properties, these pollutants are endocrine disruptors. In this review, an overview of the world's shrimp market, pollutants legislation and values found in shrimp samples will be discussed. Shrimps analysed from all continents showed the presence of contaminants, Asia being the continent with the highest values reported. The concentration values reached a maximum of 26100 ng/g wet weight (ww) for OCPs, of 226.45 ng/g ww for BFRs, of 12.1 ng/g ww for SMs and of 50650 ng/g ww for PAHs. Exposure data and risk, taken from different studies, are very variable and indicate that shrimp's consumption may represent a risk especially in certain geographic areas.

ACS Style

Maria Luz Maia; Sara Sousa; Luísa Correia-Sá; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. Organochlorine pesticides, brominated flame retardants, synthetic musks and polycyclic aromatic hydrocarbons in shrimps. An overview of occurrence and its implication on human exposure. Heliyon 2020, 6, 1 .

AMA Style

Maria Luz Maia, Sara Sousa, Luísa Correia-Sá, Cristina Delerue-Matos, Conceição Calhau, Valentina Fernandes Domingues. Organochlorine pesticides, brominated flame retardants, synthetic musks and polycyclic aromatic hydrocarbons in shrimps. An overview of occurrence and its implication on human exposure. Heliyon. 2020; 6 (9):1.

Chicago/Turabian Style

Maria Luz Maia; Sara Sousa; Luísa Correia-Sá; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. 2020. "Organochlorine pesticides, brominated flame retardants, synthetic musks and polycyclic aromatic hydrocarbons in shrimps. An overview of occurrence and its implication on human exposure." Heliyon 6, no. 9: 1.