In India, the per capita availability of water is projected to be 1465 m³ and 1235 m³ by the years 2025 and 2050, respectively, and hence, India would be a water-stressed country as per the United Nations’ standard of less than 1700 m³ per capita water availability. India is predominantly an agricultural-dominant country. Rainfed agriculture in the country contributes 40% of food grain production and supports half of the human population and two-thirds of the livestock population. The country has 15 different agro-climatic zones, and each agro-climatic region has its own constraints of water availability and management along with the potential for their optimum utilization. Such situations warrant the formulation of regional-level strategies. Efforts were made to integrate and evaluate the feasibility of water harvesting and its utilization at twelve different sites representing six different agro-climatic conditions spanning pan India. It was found that water harvesting through tanks/ponds is a feasible approach and can increase the crop production as well as diversification. The results reveal that the range of crop diversification index increased from 0.49–0.85 to 0.65–0.98; the crop productivity index increased from 0.28–0.66 to 0.66–0.90; the cultivated land utilization index increased from 0.05–0.69 to 0.34–0.84; and the crop water productivity index increased from 0.20–0.51 to 0.56–0.96, among other production and diversification indices, due to additional water availability through rainwater harvesting intervention. Moreover, the gross return increased from INR 43,768–704,356 to INR 220,840–1,469,108 ha⁻¹, representing a 108 to 400% increase in the returns due to the availability of water. The findings of this study suggest that the water harvesting in small ponds/tanks is economical and feasible, requires less technological intervention, and increases crop diversification in all the studied agro-climatic conditions, and hence, the same needs to be encouraged in the rainfed areas of the country.

Pulses are a key source of dietary proteins in human nutrition. Despite several efforts to increase the production, various constraints, such as biotic and abiotic factors, threaten pulse production by various means. Bruchids (Callosobruchus spp.) are the serious issue of concern, particularly in storage conditions. Understanding host–plant resistance at morphological, biochemical and molecular levels is the best way to minimize yield losses. The 117 mungbean (Vigna radiata L. Wilczek) genotypes, including endemic wild relatives, were screened for resistance against Callosobruchus chinensis; among them, two genotypes, PRR 2008-2 and PRR 2008-2-sel, which belong to V. umbellata (Thumb.), were identified as highly resistant. The expression of antioxidants in susceptible and resistant genotypes revealed that the activity of phenylalanine ammonia lyase (PAL) was upregulated in the highly resistant wild Vigna species and lower in the cultivated susceptible genotypes, along with other biomarkers. Further, the SCoT-based genotyping revealed SCoT-30 (200 bp), SCoT-31 (1200 bp) and SCoT-32 (300 bp) as unique amplicons, which might be useful for developing the novel ricebean-based SCAR markers to accelerate the molecular breeding programme.

Mungbean is known to be susceptible to waterlogging (WL) stress. Some of the wild species have the potential to tolerate this through various physiological and molecular mechanisms. Auxin Response Factor (ARF) and Auxin/Indole Acetic Acid (AUX/IAA), an early responsive gene family, has multiple functions in growth, development, and stress tolerance. Here, we report the first comprehensive analysis of the ARF and AUX/IAA gene family in mungbean. A total of 26 ARF and 19 AUX/IAA genes were identified from the mungbean genome. The ARF and AUX/IAA candidates were clearly grouped into two major clades. Further, the subgrouping within the major clades indicated the presence of significant diversity. The gene structure, motif analysis, and protein characterization provided the clue for further fundamental research. Out of the10 selected candidate genes, VrARF-5, VrARF-11, VrARF-25, and VrAUX/IAA-9 were found to significantly multiple-fold gene expression in the hypocotyl region of WL-tolerant wild relatives (PRR 2008-2) provides new insight into a role in the induction of lateral root formation under WL stress. The analysis provides an insight into the structural diversity of ARF and AUX/IAA genes in mungbean. These results increase our understanding of ARF and AUX/IAA genes and therefore offer robust information for functional investigations, which can be taken up in the future and will form a foundation for improving tolerance against waterlogging stress.