Astaxanthin is a natural lipid-soluble and red-orange carotenoid. Due to its strong antioxidant property, anti-inflammatory, anti-apoptotic, and immune modulation, astaxanthin has gained growing interest as a multi-target pharmacological agent against various diseases. In the current review, the anti-inflammation mechanisms of astaxanthin involved in targeting for inflammatory biomarkers and multiple signaling pathways, including PI3K/AKT, Nrf2, NF-κB, ERK1/2, JNK, p38 MAPK, and JAK-2/STAT-3, have been described. Furthermore, the applications of anti-inflammatory effects of astaxanthin in neurological diseases, diabetes, gastrointestinal diseases, hepatic and renal diseases, eye and skin disorders, are highlighted. In addition to the protective effects of astaxanthin in various chronic and acute diseases, we also summarize recent advances for the inconsistent roles of astaxanthin in infectious diseases, and give our view that the exact function of astaxanthin in response to different pathogen infection and the potential protective effects of astaxanthin in viral infectious diseases should be important research directions in the future.

East Lake in Wuhan, China, harbors a high number of freshwater fish species of great conservation value, concurrently serving as vital resources for local livelihoods. However, the ecosystem is threatened by an array of anthropogenic activities, thus requiring consistent monitoring of the local fish community to enable more efficacious conservation management. In place of conventional surveying methods, we undertook the first analysis of the fish distribution within East Lake via metabarcoding of environmental DNA (eDNA). The accuracy and efficacy of eDNA metabarcoding rely heavily upon selecting an appropriate primer set for PCR amplification. Given the varying environmental conditions and taxonomic diversity across distinct study systems, it remains a challenge to propose an optimal genetic marker for universal use. Thus, it becomes necessary to select PCR primers suitable for the composition of fish in the East Lake. Here, we evaluated the performance of two primer sets, Mifish-U and Metafish, designed to amplify 12S rRNA barcoding genes in fishes. Our results detected a total of 116 taxonomic units and 51 fish species, with beta diversity analysis indicating significant differences in community structure diversity between the six sampling locations encompassing East Lake. While it was difficult to accurately compare the species-level discriminatory power and amplification bias of the two primers, Mifish outperformed Metafish in terms of taxonomic specificity for fish taxa and reproducibility. These findings will assist with primer selection for eDNA-based fish monitoring and biodiversity conservation in the East Lake and other freshwater ecosystems.

Correlation studies about NOD1 and histones have not been reported. In the present study, we report the functional correlation between NOD1 and the histone H2A variant in response to Streptococcus agalactiae infection. In zebrafish, NOD1 deficiency significantly promoted S. agalactiae proliferation and decreased larval survival. Transcriptome analysis revealed that the significantly enriched pathways in NOD1^−/− adult zebrafish were mainly involved in immune and metabolism. Among 719 immunity-associated DEGs at 48 hpi, 74 DEGs regulated by NOD1 deficiency were histone variants. Weighted gene co-expression network analysis identified that H2A, H2B, and H3 had significant associations with NOD1 deficiency. Above all, S. agalactiae infection could induce the expression of intracellular histone H2A, as well as NOD1 colocalized with histone H2A, both in the cytoplasm and cell nucleus in the case of S. agalactiae infection. The overexpression of H2A variants such as zfH2A-6 protected against S. agalactiae infection and could improve cell survival in NOD1-deficient cells. Furthermore, NOD1 could interact with zfH2A-6 and cooperate with zfH2A-6 to inhibit the proliferation of S. agalactiae. NOD1 also showed a synergetic effect in inducing the expression of many antibacterial genes, especially antibacterial pattern recognition receptors PGRP2, PGRP5, and PGRP6. Collectively, these results firstly highlight the roles of NOD1 deficiency in the regulation of immune-related and metabolic pathways, and the correlation between zebrafish NOD1 and histone H2A variant in the defense against S. agalactiae infection.

East Lake in Wuhan, China, harbors a high number of freshwater fish species of great conservation value, concurrently serving as vital resources for local livelihoods. However, the ecosystem is threatened by an array of anthropogenic activities, thus requiring consistent monitoring of the local fish community to enable more efficacious conservation management. In place of conventional surveying methods, we undertook the first analysis of the fish distribution within East Lake via metabarcoding of environmental DNA (eDNA). The accuracy and efficacy of eDNA metabarcoding relies heavily upon selecting an appropriate primer set for PCR amplification. Given the varying environmental conditions and taxonomic diversity across distinct study systems, it remains a challenge to propose an optimal genetic marker for universal use. Thus, it becomes necessary to select PCR primers suitable for the composition of fish in the East Lake. Here, we evaluated the performance of two primer sets, Mifish-U and Metafish, designed to amplify 12S rRNA barcoding genes in fishes. Our results detected a total of 116 taxonomic units and 51 fish species, with beta diversity analysis indicating significant differences in community structure diversity between the 6 sampling locations encompassing East Lake. While it was difficult to accurately compare the species-level discriminatory power and amplification bias of the two primers, Mifish outperformed Metafish in terms of taxonomic specificity for fish taxa and reproducibility. These findings will assist with primer selection for eDNA-based fish monitoring and biodiversity conservation in the East Lake and other freshwater ecosystems.

The aquatic environments of main stems in large rivers and their connected lakes exhibit significant disparities under human activities. Fish are crucial for sustaining the structure and function of aquatic ecosystems as high-level predators. This study investigated fish communities in 192 samples from lakes and rivers across the Yangtze river (YR) basin utilizing environmental DNA (eDNA) technology. Additionally, the environmental variable impact on fish biodiversity in these two aquatic environments was uncovered. Herein, we identified approximately 230 fish taxa in this basin, with lakes and rivers comprising both prevalent and habitat-specific species. Water quality played different roles in affecting fish diversity in these two water systems. The geography traits, including Longitude, Latitude, and Altitude, as well as the water traits conductivity (CD), demonstrated the variance in fish diversity and community composition in both rivers and lakes. The human activity factors, including permanganate index (PMI), chlorophyll-a (CHLA), and SiO₂, elucidated much more variance in fish diversity and community composition in lakes. These findings suggested that human activity factors exert a more significant influence on fish diversity within lakes compared to rivers. Our outcomes document the complex impacts of water quality on fish diversity in different aquatic habitats of the YR basin and emphasize the distinctive considerations required to protect aquatic biodiversity in this basin. However, it should be noted that eDNA technology provides only a single snapshot of community composition. This method possesses limitations common to all approaches (e.g., detection gaps for certain taxa) as well as inherent biases (such as the difficulty in accurately reflecting the abundance and demographic structure of detected species).

The aquatic environments of main stems in large rivers and their connected lakes exhibit significant disparities under human activities. Fish, the top predators in aquatic ecosystems, have a crucial function in preserving the structure and functioning of these ecosystems. This study investigated fish communities in 192 samples from lakes and rivers across the Yangtze River (YR) basin utilizing environmental DNA (eDNA) technology. Additionally, the environmental variable impact on fish biodiversity in these two aquatic environments was uncovered. Herein, we identified approximately 230 fish species in this basin, with lakes and rivers comprising both prevalent and habitat-specific species. Water quality played different roles in affecting fish diversity in these two water systems. The geography traits, including Longitude, Latitude, and Altitude, as well as the water traits conductivity (CD), demonstrated the variance in fish diversity and community composition in both Rivers and Lakes. The human activity factors, including PMI, CHLA, and SiO2, elucidated much more variance in fish diversity and community composition in Lakes. These findings suggested that human activity factors exert a more significant influence on fish diversity within Lakes compared to Rivers. Our outcomes document the complex impacts of water quality on fish diversity in different aquatic habitats of the YR basin and emphasize the distinctive considerations required to protect aquatic biodiversity in this basin.