Carob (Ceratonia siliqua L.) is a tree crop cultivated extensively in the eastern Mediterranean regions but that has become naturalized in other regions as well. The present study focused on the green synthesis of zinc oxide nanoparticles (ZnONPs) from Carob and their evaluation for antimicrobial activity in bacteria and fungi. The synthesized ZnONPs showed strong antibacterial activity against Staphylococcus aureus ATCC 25 923 (92%). The NPs inhibited the growth of pathogenic yeast strains, including Candida albicans ATCC90028, Candida krusei ATCC6258, and Candida neoformans ATCC14116, by 90%, 91%, and 82%, respectively, compared to the control. Fungal inhibition zones with the ZnONPs were 88.67% and 90%, respectively, larger for Aspergillus flavus 15UA005 and Aspergillus fumigatus ATCC204305, compared to control fungal growth. This study provides novel information relevant for plant-based development of new and potentially antimicrobial ZnONPs based on extracts. In particular, the development and application of phytogenic nanoparticles enhances the biocompatibility of nano-scale materials, thereby allowing to tune effects to prevent adverse outcomes in non-target biological systems.

In contemporary times, the sustained aspiration of bioengineering and biomedical applications is the progressive advancement of materials characterized by biocompatibility and biodegradability. The investigation of the potential applications of polymers as natural and non-hazardous materials has placed significant emphasis on their physicochemical properties. Thus, this study was designed to investigate the potential of gelatin–chitosan–moringa leaf extract (G–CH–M) as a novel biomaterial for biomedical applications. The wound-dressing G–CH–M biopolymer was synthesized and characterized. The blood haemolysis, anti-inflammatory, antioxidant, and antibacterial activities of the biopolymer were investigated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial isolates. Our results showed that S. aureus swarming motility was drastically affected. However, the biopolymer had no significant effect on the swarming motility of E. coli. In addition, the biopolymer showed high antibacterial capacities, especially against S. aureus. Plasmid DNA was observed to be effectively protected from oxidative stresses by the biopolymer. Furthermore, the biopolymer exhibited greatly suppressed haemolysis (lower than 2%), notwithstanding the elevated concentration of 50 mg/mL. These results indicated that this novel biopolymer formulation could be further developed for wound care and contamination prevention.

Camel milk industrialization faces technological problems related to the presence of colostrum in milk. The determination of color parameters may serve to differentiate between colostrum and milk. This work aimed to study the relationship between the chemical composition of camel colostrum and milk and their colors. Samples of colostrum were collected at 2, 12, 24, 48, 72, 96, 120, 144, 168, and 360 h postpartum (n = 16), and their physicochemical properties (pH, acidity, viscosity, color, dry matter, ash, protein, and fat) were analyzed. The results show that all the components decreased during the first 3 days except fat. The content of this later increased from zero in the three sampling on the first day (2, 12, and 24 h) to 1.92 ± 0.61% at 48 h postpartum. The amount of total dry matter and protein decreased from 20.95 ± 3.63% and 17.43 ± 4.28% to 13.05 ± 0.81% and 3.71 ± 0.46%, respectively, during the first 7 days postpartum. There was a weak correlation between the brightness (L*) of the camel milk and its contents of dry matter, protein, and fat; however, these parameters were strongly correlated with redness (a*) and yellowness (b*). Ash content was poorly correlated with the color parameters. Hence, the measurement of the color parameters of camel colostrum and milk can be a new tool to evaluate their quality.

Faba bean (Vicia Faba var. Minor) is of great importance as it is commonly used as an excellent protein source in food and feed. In Tunisia, the wide variability among local genetic resources might be valorized by preservation and breeding programs. For that, the knowledge of the diversity within this crop and its distribution across the oasis could be of great help in managing and improving its germplasm. The objectives of the present study were to assess the phenotypic diversity within a germplasm of 23 populations of local faba bean cropped in Tunisian arid regions. Characterization was undertaken based on 29 parameters related to seeds, plant growth, flowers, and pods characteristic. This agro-morphological characterization was carried out based on UPOV and Bioversity International descriptors. Crude protein (CP), neutral and acid detergent fibers (NDF, ADF), and in vitro dry/organic matter digestibility (IVDMD/IVOMD) parameters were also analyzed. Results revealed a considerable genetic variability for most of the agro-morphological parameters. In fact, significant differences (p<0.05) were revealed by ANOVA for the majority of the analyzed quantitative traits. The coefficient of variation, used as a homogeneity index, was above 1.44 % for all characters, which ensures the predominance of genetic components in the differences among populations. The overall variability was analyzed via multivariate and dimension reduction approaches relatively using hierarchical clustering and PCA methods, in order to classify populations into relatively homogenous groups after the identification of the major traits contributing to the overall diversity. The superior populations with the best precocity (95 DAS), with high total yielding per plant (500 g), and having the highest digestibility (96.5%), the highest CP content (29.6%), and the least NDF content (44.5%) were identified. This assessment of traits diversity can assist breeders to manage and to valorize populations with desirable characteristics to be used in various breeding programs.

Traditional sun-dried merguez is an authentic Tunisian dried sausage made with a large number of spices and herbs, which was reformulated in this study with camel meat and hump fat and dried as in the artisanal process. This research studied the physicochemical, microbiological, and chemical compositional changes that occurred in fresh camel merguez (FCM) after 12 days of drying to achieve traditional dried camel merguez (DCM). The results showed significant weight loss (54.1%), as well as significant decreases in pH (5.20–4.97), moisture (60.5–12.3%), and water activity (0.986–0.673). These results and the acceptable microbiological quality of DCM can explain the safety of traditionally practiced long-term storage at room temperature. All chemical compositions increased upon drying. The composition of DCM included several organic acids, mainly lactate (2820 mg.kg⁻¹); diverse unsaturated fatty acids, in particular oleic acid (33.2%); and various minerals, specifically iron (8 mg per 100 g), in addition to volatile compounds impacted by herbs and spices rich in terpenes (56.3%). These results can be useful for investing in indigenous products and promoting the exploitation of camel meat.