We performed an integrative transcriptomic in silico analysis using lung adenocarcinoma A549 cells treated with the neddylation inhibitor MLN4924 and the gefitinib-resistant PC9 cell line (PC9GR). We focused on the transcriptional effects of the top differentially expressed ncRNA biotypes and their correlating stemness factors. Interestingly, MLN4924-treated cells showed a significant upregulation of mRNAs involved in carcinogenesis, cell attachment, and differentiation pathways, as well as a parallel downregulation of stemness maintenance and survival signaling pathways, an effect that was inversely observed in PC9GR cells. Moreover, we found that stemness factor expression could be contrasted by selected up-regulated ncRNAs upon MLN4924 treatment in a dose and time-independent manner. Furthermore, upregulated miRNAs and lncRNA-targeted mRNAs showed an evident enrichment of proliferation, differentiation, and apoptosis pathways, while downregulated ncRNA-targeted mRNAs were implicated in stem cell maintenance. Finally, our results proved that stemness (KLF4 and FGFR2) and epithelial–mesenchymal transition (ZEB2, TWIST2, SNAI2, CDH2, and VIM) factors, which are highly expressed in PC9GR cells compared to gefitinib-sensitive PC9 cells, could be abrogated with the neddylation inhibitor MLN4924 mainly through activation of epithelial differentiation pathways, thus exerting a protective role in lung cancer cells and chemosensitivity against lung tumorigenic transformation.

Indigenous people from the American continent, or Native Americans, are underrepresented in the collective genomic knowledge. A minimal percentage of individuals in international databases belong to these important minority groups. Yet, the study of native American genomics is a growing field. In this work, we reviewed 56 scientific publications where ancient or contemporary DNA of Native Americans across the continent was studied by array, whole-exome, or whole-genome technologies. In total, 13,706 native Americans have been studied with genomic technologies, of which 1292 provided whole genome samples. Data availability is lacking, with barely 3.6% of the contemporary samples clearly accessible for further studies; in striking contrast, 96.3% of the ancient samples are publicly available. We compiled census data on the home countries and found that 607 indigenous groups are still missing representation in genomic datasets. By analyzing authorship of the published works, we found that there is a need for more involvement of the home countries as leads in indigenous genomic studies. We provide this review to aid in the design of future studies that aim to reduce the missing diversity of indigenous Americans.

La contaminación del aire es un problema global con graves consecuencias para la salud humana. Los contaminantes como el polvo, el humo o los gases tóxicos afectan la esperanza de vida e incrementan la incidencia de enfermedades respiratorias, cardiovasculares y diversos tipos de cáncer. Las ciudades, con su alta actividad industrial y tráfico vehicular, son especialmente vulnerables a estos efectos. Además, la contaminación atmosférica puede desplazarse a través de fronteras, afectando comunidades más allá de su origen, lo que convierte la protección del aire en una responsabilidad compartida. Para abordar esta problemática, se han implementado tecnologías y políticas de mitigación, como la prohibición de clorofluorocarbonos, la reducción de partículas emitidas por plantas de energía y la adopción de convertidores catalíticos en vehículos. Estos avances demuestran que mejorar la calidad del aire es posible mediante esfuerzos coordinados. Investigaciones en ciencias ómicas, como la genómica y epigenómica, ayudan a entender cómo los contaminantes afectan la salud a nivel molecular, permitiendo identificar biomarcadores de exposición y diseñar intervenciones preventivas y personalizadas. Este artículo examina la situación en la ciudad de Puebla, México, donde el crecimiento urbano y la actividad industrial elevan las emisiones de PM2.5 y PM10, partículas vinculadas con enfermedades crónicas. Se analizan las tendencias de estas emisiones y los esfuerzos locales e internacionales de mitigación. La meta es ofrecer una visión integral sobre el impacto de la contaminación del aire en la salud y destacar la urgencia de fortalecer las estrategias de control ambiental en áreas vulnerables.