In this study, we conducted an exploration of blood collection, hematological and biochemical comparative analysis of the unique vitreous humor and blood of the celestial-eye goldfish (CE) (Carassius auratus), and obtained hematological and biochemical indicators for healthy celestial goldfish blood. After exploring the optimal method for collecting blood and ocular fluid, blood and vitreous humor samples were drawn from healthy CE, and an automatic hematology analyzer was used to measure 17 physiological indicators in the blood and vitreous humor. Additionally, 20 biochemical indices were measured by an automatic biochemical analyzer. Experiments were also conductedto evaluate the effect of blood and vitreous humor collection on the subsequent survival rate of the CE. The results showed that the blood of CE exhibited values for 17 hematological indicators, including red blood cells (mean value: 2.19 × 10¹²/L), white blood cells (mean value: 62.21 × 10⁹/L), hemoglobin (mean value:138.25 g/L), and no eosinophils or basophils. We also found that the levels of aspartate amino transferase (AST) and glutamyl transpeptidase (GGT) in the vitreous humor of CE were significantly higher than those in the serum (p < 0.01). No cells were detected in the vitreous humor of CE, and there was no significant difference in survival rate compared to normal goldfish after extracting blood and vitreous humor from CE (p > 0.05). This study provides fundamental data for the cultivation of healthy goldfish, the development of goldfish hematology, and further research on the development and formation of CE’s unique eye traits.

The aim of this study was to examine and understand the physiological and biochemical components of the blood and vitreous humor of goldfish (Carassius auratus). Blood was drawn from the arterial (static) line and vitreous humour was extracted using a syringe from healthy goldfish. An automatic haematology analyzer was used to detect 17 physiological indicators, including white blood cells and red blood cells. At the same time, 20 biochemical indexes, including albumin, calcium, and glutamic pyruvic transaminase, were found in the automatic biochemical analyzer. Experiments were also conducted on the effect of blood and vitreous fluid collection on the subsequent survival rate of the celestial goldfish. The findings demonstrated that there were values for 17 physiological indicators in the blood of the goldfish, including red blood cells (2.19 × 1012), white blood cells (62.21 × 109), hemoglobin (138.25g/L), and no eosinophils or basophils. However, no data for 17 physiological indications of vitreous humor were discovered. The quantities of total protein, glutamic oxaloacetic transaminase, and potassium in the vitreous humor were substantially lower than those in the serum (P < 0.01) according to the results of 20 biochemical indexes in the serum and vitreous humor of goldfish. Alkaline phosphatase, chlorine, and creatinine between vitreous humor and serum did not differ significantly(P > 0.05). The levels of glutamic pyruvic transaminase and γ-glutamyl transpeptide in vitreous humor were significantly higher than those in serum (P < 0.01). This experiment provides basic datas for the healthy culture of goldfish and the perfection of hematology in goldfish, and the results will be convenient for the further study of development and formation of celestial eye traits.

This study layed the groundwork for the creation of haploid and triploid celestial goldfish, presenting essential data derived from preliminary investigations. The research delved into three main areas: the short-term preservation of celestial goldfish gametes, the interaction between oocytes and foreign sperm, and the effects of temperature on fertilized eggs concerning hatching rates and late-stage ocular development. Initially, the study explored the optimal semen dilution ratio for celestial goldfish under microscopic examination. And the hybridization of the largemouth bass and celestial goldfish was investigated: largemouth bass sperm was crossbred with celestial goldfish eggs, and it was found that their sperm could not stimulate the development of celestial eye eggs. At last, celestial goldfish fertilized eggs were stimulated at 4 °C and −20 °C, respectively, to observe their impact on the hatching rate and later celestial eye rate. The results revealed no significant differences in hatching rate and celestial eye rate between the cold stimulus groups and the control group, but numerically, the 4 °C cold stimulation reduced the celestial eye rate of celestial goldfish fertilized eggs. The research provided fundamental data for artificial breeding and hybridization experiments in celestial goldfish.