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Carlo Mazzetti; Emanuele Ferri; Monica Pozzi; Massimo Labra. Author Correction: Quantification of the content of cannabidiol in commercially available e-liquids and studies on their thermal and photo-stability. 2021, 11, 1 .
AMA StyleCarlo Mazzetti, Emanuele Ferri, Monica Pozzi, Massimo Labra. Author Correction: Quantification of the content of cannabidiol in commercially available e-liquids and studies on their thermal and photo-stability. . 2021; 11 ():1.
Chicago/Turabian StyleCarlo Mazzetti; Emanuele Ferri; Monica Pozzi; Massimo Labra. 2021. "Author Correction: Quantification of the content of cannabidiol in commercially available e-liquids and studies on their thermal and photo-stability." 11, no. : 1.
Sub-Saharan African crop production largely relies on smallholder farms, located both in urban and agricultural landscapes. In this context, the investigation of plant and pollinator diversity and their interactions is of primary importance since both these factors are threatened by land use intensification and the consequent loss of natural habitats. In this study, we evaluated for the first time how plant and pollinator insect assemblages and interactions in Sub-Saharan farming conditions are shaped by land use intensification. To do that, we complemented biodiversity field surveys in Northern Tanzania with a modern DNA metabarcoding approach to characterize the foraged plants and thus built networks describing plant-pollinator interactions at the individual insect level. Moreover, we coupled this information with quantitative traits of landscape composition and floral availability surrounding each farm. We found that pollinator richness decreased with increasing impervious and agricultural cover in the landscape, whereas the flower density at each farm correlated with pollinator richness. The intensification of agricultural land use and urbanization correlated with a higher foraging niche overlap among pollinators due to convergence of individuals’ flower visiting strategies. Furthermore, within farms, the higher availability of floral resources drove lower niche overlap among individuals, while a greater flower visitors abundance shaped higher generalization at the networks level (H2′), possibly due to increased competition. These mechanistic understandings leading to individuals’ foraging niche overlap and generalism at the network level, could imply stability of interactions and of the pollination ecosystem service. Our integrative survey proved that plant-pollinator systems are largely affected by land use intensification and by local factors in smallholder farms of Sub-Saharan Africa. Thus, policies promoting nature-based solutions, among which the introduction of more pollinator-friendly practices by smallholder farmers, could be effective in mitigating the intensification of both urban and rural landscapes in this region, as well as in similar Sub-Saharan contexts.
Nicola Tommasi; Paolo Biella; Lorenzo Guzzetti; Julius Vincent Lasway; Henry Kenneth Njovu; Andrea Tapparo; Giulia Agostinetto; Marcell Karl Peters; Ingolf Steffan-Dewenter; Massimo Labra; Andrea Galimberti. Impact of land use intensification and local features on plants and pollinators in Sub-Saharan smallholder farms. Agriculture, Ecosystems & Environment 2021, 319, 107560 .
AMA StyleNicola Tommasi, Paolo Biella, Lorenzo Guzzetti, Julius Vincent Lasway, Henry Kenneth Njovu, Andrea Tapparo, Giulia Agostinetto, Marcell Karl Peters, Ingolf Steffan-Dewenter, Massimo Labra, Andrea Galimberti. Impact of land use intensification and local features on plants and pollinators in Sub-Saharan smallholder farms. Agriculture, Ecosystems & Environment. 2021; 319 ():107560.
Chicago/Turabian StyleNicola Tommasi; Paolo Biella; Lorenzo Guzzetti; Julius Vincent Lasway; Henry Kenneth Njovu; Andrea Tapparo; Giulia Agostinetto; Marcell Karl Peters; Ingolf Steffan-Dewenter; Massimo Labra; Andrea Galimberti. 2021. "Impact of land use intensification and local features on plants and pollinators in Sub-Saharan smallholder farms." Agriculture, Ecosystems & Environment 319, no. : 107560.
Corchorus olitorius L. is an African leafy vegetable of high nutritional interest. To assess its agricultural suitability to sustainable cultivation conditions and its potential benefits for human nutrition, its phytochemical content in response to conservation agriculture practices [i.e., no-tillage (NT) and cover crop maintenance] and low water regime were evaluated and compared with response under conventional agriculture management. Hydric stress and NT did not affect the content of antioxidant metabolites, compared to conventional agricultural practices. In both conditions, leaves were found to be a great source of phenolic compounds. The effect of these phenolic fractions was assessed on two colon cell phenotypes to evaluate putative nutraceutical properties. Polyphenol-enriched extracts (PEEs) displayed selective cytotoxic activities against tumor Caco-2 cells but not on the healthy CCD841 line. PEEs were able to trigger oxidative stress and to inhibit the activity of glutathione-independent antioxidant enzymes on Caco-2 cells. C. olitorius showed to be a promising crop for improving both agricultural sustainability and health benefits due to the great amount of antioxidant compounds in leaves, whose occurrence is not altered by stressful farming conditions. Given its high adaptability, the cultivation of this crop is therefore recommendable also in the Mediterranean Basin.
Lorenzo Guzzetti; Davide Panzeri; Marynka Ulaszewska; Grazia Sacco; Matilde Forcella; Paola Fusi; Nicola Tommasi; Andrea Fiorini; Luca Campone; Massimo Labra. Assessment of Dietary Bioactive Phenolic Compounds and Agricultural Sustainability of an African Leafy Vegetable Corchorus olitorius L. Frontiers in Nutrition 2021, 8, 1 .
AMA StyleLorenzo Guzzetti, Davide Panzeri, Marynka Ulaszewska, Grazia Sacco, Matilde Forcella, Paola Fusi, Nicola Tommasi, Andrea Fiorini, Luca Campone, Massimo Labra. Assessment of Dietary Bioactive Phenolic Compounds and Agricultural Sustainability of an African Leafy Vegetable Corchorus olitorius L. Frontiers in Nutrition. 2021; 8 ():1.
Chicago/Turabian StyleLorenzo Guzzetti; Davide Panzeri; Marynka Ulaszewska; Grazia Sacco; Matilde Forcella; Paola Fusi; Nicola Tommasi; Andrea Fiorini; Luca Campone; Massimo Labra. 2021. "Assessment of Dietary Bioactive Phenolic Compounds and Agricultural Sustainability of an African Leafy Vegetable Corchorus olitorius L." Frontiers in Nutrition 8, no. : 1.
The COVID-19 emergency and the consequent social distancing requirements have caused major disruptions in daily food-related practices at the household level. In this paper, we evaluate the transformations that occurred in the daily nutritional choices and behaviors of a convenience sample (n = 2288) of Italian residents during the first nation-wide lockdown (March–May 2020) to assess the impact on the health and socio-environmental sustainability of their diets. Results portray a scenario of wide-spread change, especially in relation to the quantity of daily food consumed, the composition of diets and the time and commitment devoted to home-cooking, with young individuals emerging as the most impacted generational cohort. Through the construction of an indicator for healthy–sustainable transition (HST index), we demonstrate that such changes unfold on a gradient, revealing that while for many respondents lockdown nutrition implied overeating and weight gain, a substantial segment of the population conversely improved the healthiness and sustainability of their daily nutritional patterns. In this sense, improvements are associated with young age, socio-economic status, frequency and enjoyment of cooking-from-scratch and, more generally, an attentive attitude towards the quality, provenance and materiality of food that, in turn, the COVID-19 crisis appears to have re-kindled. We conclude by highlighting five areas of institutional intervention (i.e., young people, time, tools, food supply at work, and local food chains) on which to focus in order to ensure the current crisis does not represent a missed opportunity for creating the necessary conditions for sustainable food production and consumption to take hold as the ‘new’ normal in the post-pandemic era.
Raffaele Matacena; Mariangela Zenga; Marco D’Addario; Silvia Mari; Massimo Labra. COVID-19 as an Opportunity for a Healthy-Sustainable Food Transition. An Analysis of Dietary Transformations during the First Italian Lockdown. Sustainability 2021, 13, 5661 .
AMA StyleRaffaele Matacena, Mariangela Zenga, Marco D’Addario, Silvia Mari, Massimo Labra. COVID-19 as an Opportunity for a Healthy-Sustainable Food Transition. An Analysis of Dietary Transformations during the First Italian Lockdown. Sustainability. 2021; 13 (10):5661.
Chicago/Turabian StyleRaffaele Matacena; Mariangela Zenga; Marco D’Addario; Silvia Mari; Massimo Labra. 2021. "COVID-19 as an Opportunity for a Healthy-Sustainable Food Transition. An Analysis of Dietary Transformations during the First Italian Lockdown." Sustainability 13, no. 10: 5661.
In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules.
Stefano Bertacchi; Stefania Pagliari; Chiara Cantù; Ilaria Bruni; Massimo Labra; Paola Branduardi. Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids. International Journal of Environmental Research and Public Health 2021, 18, 1146 .
AMA StyleStefano Bertacchi, Stefania Pagliari, Chiara Cantù, Ilaria Bruni, Massimo Labra, Paola Branduardi. Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids. International Journal of Environmental Research and Public Health. 2021; 18 (3):1146.
Chicago/Turabian StyleStefano Bertacchi; Stefania Pagliari; Chiara Cantù; Ilaria Bruni; Massimo Labra; Paola Branduardi. 2021. "Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids." International Journal of Environmental Research and Public Health 18, no. 3: 1146.
This paper intends to investigate the behaviours adopted during this lockdown period due to SARS-CoV-2, in which most individuals have found themselves forced to carry out their daily lives within domestic boundaries, and compare them with previously practiced food-related habits. The aim is to understand whether strategies of buying, preparing and consuming food have changed with respect to established habits of 'ordinary' periods, and how these choices are linked to the psychological and emotional wellbeing/feeling experienced by individuals, to the physiological well-being of individuals and to social, environmental and economic sustainability. The analysis is based on the survey carried out by the Department of Psychology in collaboration with the interdepartmental center BEST4Food of Milano-Bicocca university.
Marco D’Addario; Massimo Labra; Silvia Mari; Raffaele Matacena; Mariangela Zenga. An analysis of the transaction towards sustainable food consumption practises during the Italian lockdown for SARS-CoV-2: the experience of the Lombardy region. Proceedings e report 2021, 127, 121 -126.
AMA StyleMarco D’Addario, Massimo Labra, Silvia Mari, Raffaele Matacena, Mariangela Zenga. An analysis of the transaction towards sustainable food consumption practises during the Italian lockdown for SARS-CoV-2: the experience of the Lombardy region. Proceedings e report. 2021; 127 ():121-126.
Chicago/Turabian StyleMarco D’Addario; Massimo Labra; Silvia Mari; Raffaele Matacena; Mariangela Zenga. 2021. "An analysis of the transaction towards sustainable food consumption practises during the Italian lockdown for SARS-CoV-2: the experience of the Lombardy region." Proceedings e report 127, no. : 121-126.
Combining no-till and cover crops (NT + CC) as an alternative to conventional tillage (CT) is generating interest to build-up farming systems’ resilience while promoting climate change adaptation in agriculture. Our field study aimed to assess the impact of long-term NT + CC management and short-term water stress on soil microbial communities, enzymatic activities, and the distribution of C and N within soil aggregates. High-throughput sequencing (HTS) revealed the positive impact of NT + CC on microbial biodiversity, especially under water stress conditions, with the presence of important rhizobacteria (e.g., Bradyrhizobium spp.). An alteration index based on soil enzymes confirmed soil depletion under CT. C and N pools within aggregates showed an enrichment under NT + CC mostly due to C and N-rich large macroaggregates (LM), accounting for 44% and 33% of the total soil C and N. Within LM, C and N pools were associated to microaggregates within macroaggregates (mM), which are beneficial for long-term C and N stabilization in soils. Water stress had detrimental effects on aggregate formation and limited C and N inclusion within aggregates. The microbiological and physicochemical parameters correlation supported the hypothesis that long-term NT + CC is a promising alternative to CT, due to the contribution to soil C and N stabilization while enhancing the biodiversity and enzymes.
Eren Taskin; Roberta Boselli; Andrea Fiorini; Chiara Misci; Federico Ardenti; Francesca Bandini; Lorenzo Guzzetti; Davide Panzeri; Nicola Tommasi; Andrea Galimberti; Massimo Labra; Vincenzo Tabaglio; Edoardo Puglisi. Combined Impact of No-Till and Cover Crops with or without Short-Term Water Stress as Revealed by Physicochemical and Microbiological Indicators. Biology 2021, 10, 23 .
AMA StyleEren Taskin, Roberta Boselli, Andrea Fiorini, Chiara Misci, Federico Ardenti, Francesca Bandini, Lorenzo Guzzetti, Davide Panzeri, Nicola Tommasi, Andrea Galimberti, Massimo Labra, Vincenzo Tabaglio, Edoardo Puglisi. Combined Impact of No-Till and Cover Crops with or without Short-Term Water Stress as Revealed by Physicochemical and Microbiological Indicators. Biology. 2021; 10 (1):23.
Chicago/Turabian StyleEren Taskin; Roberta Boselli; Andrea Fiorini; Chiara Misci; Federico Ardenti; Francesca Bandini; Lorenzo Guzzetti; Davide Panzeri; Nicola Tommasi; Andrea Galimberti; Massimo Labra; Vincenzo Tabaglio; Edoardo Puglisi. 2021. "Combined Impact of No-Till and Cover Crops with or without Short-Term Water Stress as Revealed by Physicochemical and Microbiological Indicators." Biology 10, no. 1: 23.
The essential oils (EOs) of the Eurasiatic Valeriana officinalis (Vo), the Himalayan Valeriana jatamansi (Vj) and Nardostachys jatamansi (Nj), are traditionally used to treat neurological disorders. A multidisciplinary approach based on micromorphology, DNA barcoding, EOs characterization as well as evaluation of the biological effects on the nervous system was carried out. Nj showed the highest inhibitory activity on acetylcholinesterase (IC50 67.15 μg/mL), followed by Vo (IC50 127.30 μg/mL) and Vj (IC50 246.84 μg/mL). Microelectrode arrays analyses on rat cortical neurons revealed stronger inhibition by Nj (IC50 18.8 and 11.1 μg/mL) and Vo (16.5 and 22.5 μg/mL), compared with Vj (68.5 and 89.3 μg/mL). These results could be related to the different EOs composition and in particular to the different content of oxygenated compounds such as aldehydes and ketones, which represents a discriminating factor in determining the order of potency. In conclusion, this multidisciplinary approach could be a useful tool to quickly discriminate these three plant species and avoid adulterations.
Laura Cornara; Gabriele Ambu; Domenico Trombetta; Marcella Denaro; Susanna Alloisio; Jessica Frigerio; Massimo Labra; Govinda Ghimire; Marco Valussi; Antonella Smeriglio. Multidisciplinary Screening of Three Species belonging to Caprifoliaceae Family Traditionally Used as Antidepressants. Biology and Life Sciences Forum 2020, 4, 2 .
AMA StyleLaura Cornara, Gabriele Ambu, Domenico Trombetta, Marcella Denaro, Susanna Alloisio, Jessica Frigerio, Massimo Labra, Govinda Ghimire, Marco Valussi, Antonella Smeriglio. Multidisciplinary Screening of Three Species belonging to Caprifoliaceae Family Traditionally Used as Antidepressants. Biology and Life Sciences Forum. 2020; 4 (1):2.
Chicago/Turabian StyleLaura Cornara; Gabriele Ambu; Domenico Trombetta; Marcella Denaro; Susanna Alloisio; Jessica Frigerio; Massimo Labra; Govinda Ghimire; Marco Valussi; Antonella Smeriglio. 2020. "Multidisciplinary Screening of Three Species belonging to Caprifoliaceae Family Traditionally Used as Antidepressants." Biology and Life Sciences Forum 4, no. 1: 2.
Omics tools offer the opportunity to characterize and trace traditional and industrial fermented foods. Bioinformatics, through machine learning, and other advanced statistical approaches, are able to disentangle fermentation processes and to predict the evolution and metabolic outcomes of a food microbial ecosystem. By assembling microbial artificial consortia, the biotechnological advances will also be able to enhance the nutritional value and organoleptics characteristics of fermented food, preserving, at the same time, the potential of autochthonous microbial consortia and metabolic pathways, which are difficult to reproduce. Preserving the traditional methods contributes to protecting the hidden value of local biodiversity, and exploits its potential in industrial processes with the final aim of guaranteeing food security and safety, even in developing countries.
Andrea Galimberti; Antonia Bruno; Giulia Agostinetto; Maurizio Casiraghi; Lorenzo Guzzetti; Massimo Labra. Fermented food products in the era of globalization: tradition meets biotechnology innovations. Current Opinion in Biotechnology 2020, 70, 36 -41.
AMA StyleAndrea Galimberti, Antonia Bruno, Giulia Agostinetto, Maurizio Casiraghi, Lorenzo Guzzetti, Massimo Labra. Fermented food products in the era of globalization: tradition meets biotechnology innovations. Current Opinion in Biotechnology. 2020; 70 ():36-41.
Chicago/Turabian StyleAndrea Galimberti; Antonia Bruno; Giulia Agostinetto; Maurizio Casiraghi; Lorenzo Guzzetti; Massimo Labra. 2020. "Fermented food products in the era of globalization: tradition meets biotechnology innovations." Current Opinion in Biotechnology 70, no. : 36-41.
This study is aimed at valorizing artichoke (Cynara cardunculus var. scolymus L.) by-products as source of inulin, a fiber showing relevant prebiotic properties, through the realization of a waste value chain. Starting from artichoke by-products, the inulin fraction was assessed both in terms of total amount and degree of polymerization as a function of the harvest season and storage conditions. These parameters have been found significant at influencing inulin yield of extraction. For the first time, artichoke wastes were proposed to be exploited taking into account the optimal conditions to preserve their high-added chemical value. Our data suggest that Italian farms could obtain from their wastes a total amount of 16 t/year of inulin with an average polymerization degree higher than 40 and would allow the development of a circular economy process within the artichoke supply chain, by exploiting its wastes representing 70% of the total artichoke biomass.
Sofia Cavini; Lorenzo Guzzetti; Francesca Givoia; Maria Elena Regonesi; Patrizia Di Gennaro; Chiara Magoni; Luca Campone; Massimo Labra; Ilaria Bruni. Artichoke (Cynara cardunculus var. scolymus L.) by-products as a source of inulin: how to valorise an agricultural supply chain extracting an added-value compound. Natural Product Research 2020, 1 -5.
AMA StyleSofia Cavini, Lorenzo Guzzetti, Francesca Givoia, Maria Elena Regonesi, Patrizia Di Gennaro, Chiara Magoni, Luca Campone, Massimo Labra, Ilaria Bruni. Artichoke (Cynara cardunculus var. scolymus L.) by-products as a source of inulin: how to valorise an agricultural supply chain extracting an added-value compound. Natural Product Research. 2020; ():1-5.
Chicago/Turabian StyleSofia Cavini; Lorenzo Guzzetti; Francesca Givoia; Maria Elena Regonesi; Patrizia Di Gennaro; Chiara Magoni; Luca Campone; Massimo Labra; Ilaria Bruni. 2020. "Artichoke (Cynara cardunculus var. scolymus L.) by-products as a source of inulin: how to valorise an agricultural supply chain extracting an added-value compound." Natural Product Research , no. : 1-5.
The ongoing pandemic caused by the coronavirus disease 2019 (COVID-19) is literally changing the world. From December 2019 to date, more than 22 million cases have been reported worldwide and global health institutions are acting to slow down the virus transmission and are looking for possible prevention strategies in case of a new outbreak. As in other endemic or pandemic phenomena, the issues mostly covered by scientific and media attention are related to the diagnostic and therapeutic approach of COVID-19. However, a still neglected issue regards the adoption of a more systemic approach considering the close connection among the infection, the environment, and human behaviors, including the role of diet and urban management. To shed light on this issue, we brought together a faculty group involving experts in environment and biodiversity, food safety, human nutrition, and behavior, bioprospecting, as well as medical doctors having a deep knowledge of the complex historical relationship between humanity and vector-borne infections. Two main aspects emerged from the integrative overview of the current COVID-19 pandemic: (i) the scientific community should start sharing social actions and policy advocacy based on the assumption that human health strongly depends upon a sustainable exploitation of natural resources in populated areas; (ii) the specific strategic role of the cities in developing sustainable food systems and promoting healthy dietary patterns. Definitely, some priority issues should be addressed to achieve these goals, such as global efforts to increase food safety and security, which would benefit from urban and peri-urban agriculture enhancement, smallholder food producers support, and ecosystem services and local biodiversity maintenance.
Andrea Galimberti; Hellas Cena; Luca Campone; Emanuele Ferri; Mario Dell'Agli; Enrico Sangiovanni; Michael Belingheri; Michele Augusto Riva; Maurizio Casiraghi; Massimo Labra. Rethinking Urban and Food Policies to Improve Citizens Safety After COVID-19 Pandemic. Frontiers in Nutrition 2020, 7, 569542 .
AMA StyleAndrea Galimberti, Hellas Cena, Luca Campone, Emanuele Ferri, Mario Dell'Agli, Enrico Sangiovanni, Michael Belingheri, Michele Augusto Riva, Maurizio Casiraghi, Massimo Labra. Rethinking Urban and Food Policies to Improve Citizens Safety After COVID-19 Pandemic. Frontiers in Nutrition. 2020; 7 ():569542.
Chicago/Turabian StyleAndrea Galimberti; Hellas Cena; Luca Campone; Emanuele Ferri; Mario Dell'Agli; Enrico Sangiovanni; Michael Belingheri; Michele Augusto Riva; Maurizio Casiraghi; Massimo Labra. 2020. "Rethinking Urban and Food Policies to Improve Citizens Safety After COVID-19 Pandemic." Frontiers in Nutrition 7, no. : 569542.
The essential oils (EOs) of three Caprifoliaceae species, the Eurasiatic Valeriana officinalis (Vo), the Himalayan Valeriana jatamansi (Vj) and Nardostachys jatamansi (Nj), are traditionally used to treat neurological disorders. Roots/rhizomes micromorphology, DNA barcoding and EOs phytochemical characterization were carried out, while biological effects on the nervous system were assessed by acetylcholinesterase (AChE) inhibitory activity and microelectrode arrays (MEA). Nj showed the highest inhibitory activity on AChE (IC50 67.15 μg/mL) followed by Vo (IC50 127.30 μg/mL) and Vj (IC50 246.84 μg/mL). MEA analyses on rat cortical neurons, carried out by recording mean firing rate (MFR) and mean bursting rate (MBR), revealed stronger inhibition by Nj (IC50 18.8 and 11.1 μg/mL) and Vo (16.5 and 22.5 μg/mL), compared with Vj (68.5 and 89.3 μg/mL). These results could be related to different EO compositions, since sesquiterpenes and monoterpenes significantly contribute to the observed effects, but the presence of oxygenated compounds such as aldehydes and ketones is a discriminating factor in determining the order of potency. Our multidisciplinary approach represents an important tool to avoid the adulteration of herbal drugs and permits the evaluation of the effectiveness of EOs that could be used for a wide range of therapeutic applications.
Laura Cornara; Gabriele Ambu; Domenico Trombetta; Marcella Denaro; Susanna Alloisio; Jessica Frigerio; Massimo Labra; Govinda Ghimire; Marco Valussi; Antonella Smeriglio. Comparative and Functional Screening of Three Species Traditionally used as Antidepressants: Valeriana officinalis L., Valeriana jatamansi Jones ex Roxb. and Nardostachys jatamansi (D.Don) DC. Plants 2020, 9, 994 .
AMA StyleLaura Cornara, Gabriele Ambu, Domenico Trombetta, Marcella Denaro, Susanna Alloisio, Jessica Frigerio, Massimo Labra, Govinda Ghimire, Marco Valussi, Antonella Smeriglio. Comparative and Functional Screening of Three Species Traditionally used as Antidepressants: Valeriana officinalis L., Valeriana jatamansi Jones ex Roxb. and Nardostachys jatamansi (D.Don) DC. Plants. 2020; 9 (8):994.
Chicago/Turabian StyleLaura Cornara; Gabriele Ambu; Domenico Trombetta; Marcella Denaro; Susanna Alloisio; Jessica Frigerio; Massimo Labra; Govinda Ghimire; Marco Valussi; Antonella Smeriglio. 2020. "Comparative and Functional Screening of Three Species Traditionally used as Antidepressants: Valeriana officinalis L., Valeriana jatamansi Jones ex Roxb. and Nardostachys jatamansi (D.Don) DC." Plants 9, no. 8: 994.
Microalgae are a precious source of polyunsaturated fatty acids (PUFA), however extraction is difficult due to the peculiar microalgae cell structure. Here we describe a new method based on the application of chitosan nanoaggregates as CO2 responsive emulsifier, used to promote the swelling of algae cell wall and the formation of a large oil - ethanol interphase area during the ethanolysis. Tests were carried out with Pseudokirchneriella subcapitata and Nannochloropsis sp. at different biomass/ethanol/chitosan ratios. CO2 was added to trigger demulsification to promote an easy recovery of the lipid fraction. The highest yields in PUFA were obtained with Nannochloropsis sp. (207.9 mg/g of oil) using 0.4% wt of chitosan and 1:10 biomass:ethanol ratio; 43.6 mg/g of linolenic acid were obtained from Pseudokirchneriella subcapitata. Overall, because the method employs ethanol, a generally recognized as safe (GRAS) solvent, and food grade additives, it is suited for the preparation of PUFA supplements.
Francesco Saliu; Chiara Magoni; Anna Torelli; Radiana Cozza; Marina Lasagni; Massimo Labra. Omega-3 rich oils from microalgae: A chitosan mediated in situ transesterification method. Food Chemistry 2020, 337, 127745 .
AMA StyleFrancesco Saliu, Chiara Magoni, Anna Torelli, Radiana Cozza, Marina Lasagni, Massimo Labra. Omega-3 rich oils from microalgae: A chitosan mediated in situ transesterification method. Food Chemistry. 2020; 337 ():127745.
Chicago/Turabian StyleFrancesco Saliu; Chiara Magoni; Anna Torelli; Radiana Cozza; Marina Lasagni; Massimo Labra. 2020. "Omega-3 rich oils from microalgae: A chitosan mediated in situ transesterification method." Food Chemistry 337, no. : 127745.
Honey is a natural food widely consumed due to its high content in nutrients and bioactive substances. In order to prevent hive infections, xenobiotics such as pesticides and antibiotics are commonly used. Chloramphenicol (CAP) is a broad-spectrum antibiotic used to treat honeybee larvae diseases. However, CAP has toxic and nondose-dependent effects in sensitive subjects; for this reason, its use has been prohibited in food-producing animals, such as the honeybee. In this study, we proposed a rapid, simple, and cheap analytical method, based on salting-out assisted liquid-liquid extraction coupled with UHPLC MS/MS detection for the accurate determination of CAP in honey to be used in routine analyses. The parameters that influence the extraction efficiency have been optimized using an experimental design in order to maximize the recovery of the analyte by reducing the matrix effects. Therefore, the developed method was internally validated according to the 2002/657/EC Decision guidelines and applied to the analysis of 96 honey samples.
Serena Rizzo; Mariateresa Russo; Massimo Labra; Luca Campone; Luca Rastrelli. Determination of Chloramphenicol in Honey Using Salting-Out Assisted Liquid-Liquid Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry and Validation According to 2002/657 European Commission Decision. Molecules 2020, 25, 3481 .
AMA StyleSerena Rizzo, Mariateresa Russo, Massimo Labra, Luca Campone, Luca Rastrelli. Determination of Chloramphenicol in Honey Using Salting-Out Assisted Liquid-Liquid Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry and Validation According to 2002/657 European Commission Decision. Molecules. 2020; 25 (15):3481.
Chicago/Turabian StyleSerena Rizzo; Mariateresa Russo; Massimo Labra; Luca Campone; Luca Rastrelli. 2020. "Determination of Chloramphenicol in Honey Using Salting-Out Assisted Liquid-Liquid Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry and Validation According to 2002/657 European Commission Decision." Molecules 25, no. 15: 3481.
Traceability, quality and safety of edible insects are important both for the producers and the consumers. Today, alongside the burst of edible insects in western countries, we are facing a gap of knowledge of insect microbiota associated with the microbial ecosystems of insect-based products. In this context, High-Throughput DNA Sequencing (HTS) techniques can give insight into the carryover of insect microbiota into final food products. In this study, we investigated the microbiota composition of insect-based commercial food products, applying HTS techniques coupled with bioinformatic analysis. The work aimed to analyse the microbiota variability of different categories of some insect-based commercial food products made of A. domesticus (house cricket), T. molitor (mealworm beetle), and A. diaperinus (lesser mealworm or litter beetle), including commercial raw materials and processed food items, purchased via e-commerce from different companies. Our data revealed that samples cluster per insect species based on microbiota profile and preliminary results suggested that a small number of prevalent bacteria formed a “core microbiota” characterizing the products depending on the insect. This microbial signature can be recognized despite the different food processing levels, rearing conditions and selling companies. Furthermore, differences between raw and processed food made of the same insect or similar product produced by different companies was found. These results support the application of HTS analysis for studying the composition of insect-based commercial food products in a wider perspective, for food traceability and food quality control.
Jessica Frigerio; Giulia Agostinetto; Andrea Galimberti; Fabrizio De Mattia; Massimo Labra; Antonia Bruno. Tasting the differences: Microbiota analysis of different insect-based novel food. Food Research International 2020, 137, 109426 .
AMA StyleJessica Frigerio, Giulia Agostinetto, Andrea Galimberti, Fabrizio De Mattia, Massimo Labra, Antonia Bruno. Tasting the differences: Microbiota analysis of different insect-based novel food. Food Research International. 2020; 137 ():109426.
Chicago/Turabian StyleJessica Frigerio; Giulia Agostinetto; Andrea Galimberti; Fabrizio De Mattia; Massimo Labra; Antonia Bruno. 2020. "Tasting the differences: Microbiota analysis of different insect-based novel food." Food Research International 137, no. : 109426.
Cannabidiol (CBD) has become a buzzword in many products that have bloomed on the market. The scientific community and some authorities have recently raised concerns on the quality of these products. In particular, the discrepancy between the labelled and the real content of cannabidiol in liquids for e-cigarettes seems to be emerging as a major issue. Furthermore, to-date no studies have been carried out on the chemical stability upon storage of these type of products. The aim of this work was to investigate the accuracy in labelling of thirteen commercially e-liquids containing CBD and the effects of different storage conditions on their quality. The results showed that only 38% of samples were labelled within a ±10% variance from their labels. Stability tests showed a maximum degradation of CBD when samples were stored at 37 °C for 30 days with average values up to 20%. The effect of light was lower but still significant with averages values up to 15% degradation after 30 days. In conclusion, we believe that health authorities should regulate and control this market more stringently to protect customers and their health. Furthermore, our stability tests have shown that if clear indications are provided on the best storage conditions, the quality of these products can be mostly preserved.
Carlo Mazzetti; Emanuele Ferri; Monica Pozzi; Massimo Labra. Quantification of the content of cannabidiol in commercially available e-liquids and studies on their thermal and photo-stability. Scientific Reports 2020, 10, 1 -6.
AMA StyleCarlo Mazzetti, Emanuele Ferri, Monica Pozzi, Massimo Labra. Quantification of the content of cannabidiol in commercially available e-liquids and studies on their thermal and photo-stability. Scientific Reports. 2020; 10 (1):1-6.
Chicago/Turabian StyleCarlo Mazzetti; Emanuele Ferri; Monica Pozzi; Massimo Labra. 2020. "Quantification of the content of cannabidiol in commercially available e-liquids and studies on their thermal and photo-stability." Scientific Reports 10, no. 1: 1-6.
Beer is one the most consumed alcoholic beverage in the world and its contamination with mycotoxins is of public health concern. This study reports a fast and automated analytical procedure based on a multi-heart-cutting two-dimensional liquid chromatography tandem mass spectrometry method using electrospray ionization for the determination of seven mycotoxins (aflatoxins B1, B2, G2 and G1, ochratoxin A, fumonisins B1 and B2) in beers. The developed method was based on the heart-cutting 2D- HPLC technique in which only the specific portions of the first dimension, in the retention time of analytes, were transferred into the second dimension for the further separation and successive determination. The method uses two different chromatographic columns; in the first dimension, 50 μL of sample was injected on first column, and mycotoxins elution regions were collected in a loop and transferred into the second column for the separation of analytes. Each column operated in gradient elution mode in order to eliminate interfering compounds and improve separation and peak shape. After the optimization, the method has been validated according to EU regulation and finally applied for the analysis of forty beer samples collected from Italian supermarkets. Among all mycotoxins studied, fumonisins B1 was the most widely distributed in analysed beers (>21%) in the range from 0.6 to 12.3 ng mL−1. The automated methodology developed was able to determine accurately and simultaneously seven mycotoxins in beer. This provided a significant reduction of sample handle and, consequently of analysis time.
Luca Campone; Serena Rizzo; Anna Lisa Piccinelli; Rita Celano; Imma Pagano; Mariateresa Russo; Massimo Labra; Luca Rastrelli. Determination of mycotoxins in beer by multi heart-cutting two-dimensional liquid chromatography tandem mass spectrometry method. Food Chemistry 2020, 318, 126496 .
AMA StyleLuca Campone, Serena Rizzo, Anna Lisa Piccinelli, Rita Celano, Imma Pagano, Mariateresa Russo, Massimo Labra, Luca Rastrelli. Determination of mycotoxins in beer by multi heart-cutting two-dimensional liquid chromatography tandem mass spectrometry method. Food Chemistry. 2020; 318 ():126496.
Chicago/Turabian StyleLuca Campone; Serena Rizzo; Anna Lisa Piccinelli; Rita Celano; Imma Pagano; Mariateresa Russo; Massimo Labra; Luca Rastrelli. 2020. "Determination of mycotoxins in beer by multi heart-cutting two-dimensional liquid chromatography tandem mass spectrometry method." Food Chemistry 318, no. : 126496.
The need to develop experimental tools for a responsible research and innovation (RRI) framework is relevant for managing research agendas and policy making that seriously take into account the complex conditions of innovation development (linked to multidisciplinarity and interaction processes) between the researchers and their fieldwork activities. The adoption of an RRI framework is even more important for multidisciplinary and complex issues, such as the agri-food system. In this context, the SASS (Sustainable Agri-food Systems for Sustainable Development (SASS) project represents a good example for verifying the application of the RRI strategy in a varied research group committed to the development of sustainable agri-food systems in Sub-Saharan Africa. The project, which involves more than 50 researchers from different fields of knowledge and theoretical backgrounds, showed the importance of the processes of reflection, re-driving, and convergence in the definition of research objectives and strategies. This process started by experimenting with new dedicated RRI tools in order to allow interactions between the researchers, including exchanging their experience in data collection and theoretical reflection development. With respect to this analysis, it was interesting to analyze how the RRI tools and strategies have been activated between researchers and different stakeholders, generating reflections capable of re-adapting the results towards shared and accessible innovation for the extended society. Following the discussion based on the description of the SASS-RRI agenda tools and following an internal verification given from an RRI-based web survey, this contribution provides new insights, in terms of tools and strategies, to promote and refine RRI approaches. This work underlines how RRI methods have promoted internal and external interactions to connect the research objectives towards a model of open innovation.
Luca Tricarico; Andrea Galimberti; Ausilia Campanaro; Chiara Magoni; Massimo Labra. Experimenting with RRI tools to Drive Sustainable Agri-Food Research: The SASS Case Study from Sub-Saharan Africa. Sustainability 2020, 12, 827 .
AMA StyleLuca Tricarico, Andrea Galimberti, Ausilia Campanaro, Chiara Magoni, Massimo Labra. Experimenting with RRI tools to Drive Sustainable Agri-Food Research: The SASS Case Study from Sub-Saharan Africa. Sustainability. 2020; 12 (3):827.
Chicago/Turabian StyleLuca Tricarico; Andrea Galimberti; Ausilia Campanaro; Chiara Magoni; Massimo Labra. 2020. "Experimenting with RRI tools to Drive Sustainable Agri-Food Research: The SASS Case Study from Sub-Saharan Africa." Sustainability 12, no. 3: 827.
Nowadays, agriculture is facing the great challenge of climate change which puts the productivity of the crops in peril due to unpredictable rain patterns and water shortages, especially in the developing world. Besides productivity, nutritional values of the yields of these crops may also be affected, especially under low mechanization and the low water availability conditions of the developing world. Conservation agriculture (CA) is a topic of emerging interest due to the provision of adequate yields and reduced environmental impact, such as greenhouse gas emissions, by being based on three main principles: minimum soil disturbance (reduced or no tillage), cover crop maintenance, and crop rotation. The aim of this study was to assess the impact of CA management on the growth performance and the nutritional profile of cowpea (Vigna unguiculata L. Walp), a pulse of African origin, commonly known as black eye bean under field conditions. A field experiment was designed to assess the effect of conventional tillage (CT) and no-tillage (NT) combined with the usage of a set of cover crops, coupled to normal and deficient water regimes. Cowpea was revealed to be able to grow and yield comparably at each level of the treatment tested, with a better ability to face water exhaustion under CA management. After a faster initial growth phase in CT plots, the level of adaptability of this legume to NT was such that growth performances improved significantly with respect to CT plots. The flowering rate was higher and earlier in CT conditions, while in NT it was slower but longer-lasting. The leafy photosynthetic rate and the nutritional profile of beans were slightly influenced by tillage management: only total starch content was negatively affected in NT and watered plots while proteins and aminoacids did not show any significant variation. Furthermore, significantly higher carbon and nitrogen concentration occurred in NT soils especially at the topmost (0–5 cm) soil horizon. These findings confirm the capability of CA to enrich soil superficial horizons and highlight that cowpea is a suitable crop to be grown under sustainable CA management. This practice could be pivotal to preserve soils and to save agronomical costs without losing a panel of nutrients that are important to the human diet. Due to its great protein and aminoacidic composition, V. unguiculata is a good candidate for further cultivation in regions of the word facing deficiencies in the intake of such nutrients, such as the Mediterranean basins and Sub-Saharan countries.
Lorenzo Guzzetti; Andrea Fiorini; Davide Panzeri; Nicola Tommasi; Fabrizio Grassi; Eren Taskin; Chiara Misci; Edoardo Puglisi; Vincenzo Tabaglio; Andrea Galimberti; Massimo Labra. Sustainability Perspectives of Vigna unguiculata L. Walp. Cultivation under No Tillage and Water Stress Conditions. Plants 2019, 9, 48 .
AMA StyleLorenzo Guzzetti, Andrea Fiorini, Davide Panzeri, Nicola Tommasi, Fabrizio Grassi, Eren Taskin, Chiara Misci, Edoardo Puglisi, Vincenzo Tabaglio, Andrea Galimberti, Massimo Labra. Sustainability Perspectives of Vigna unguiculata L. Walp. Cultivation under No Tillage and Water Stress Conditions. Plants. 2019; 9 (1):48.
Chicago/Turabian StyleLorenzo Guzzetti; Andrea Fiorini; Davide Panzeri; Nicola Tommasi; Fabrizio Grassi; Eren Taskin; Chiara Misci; Edoardo Puglisi; Vincenzo Tabaglio; Andrea Galimberti; Massimo Labra. 2019. "Sustainability Perspectives of Vigna unguiculata L. Walp. Cultivation under No Tillage and Water Stress Conditions." Plants 9, no. 1: 48.
In the context of novel foods, a category for which the market demand is increasing worldwide, the consumption of edible insects and related insect-based products is expected to grow in the next years. Insects represent an important source of energy for the human diet but there is a lack of scientific knowledge about their processing to ensure safe food items to the consumer. In this study we adopted a combined DNA-based approach to verify the identity of the declared species in five categories of commercial insect-based products (mt COI DNA barcoding) and to characterize plant declared ingredients or contaminants (nu ITS2 DNA metabarcoding) with particular attention to putative elements of allergenic concern belonging, for example to the insect rearing substrate. Moreover, the same approach has been used to assess its sensitivity to cases of contamination and counterfeits to insect flour with low cost (and potentially allergenic) vegetable flours like wheat and soybean. Results show the success of insect DNA barcoding authentication even for highly processed products. Furthermore, the DNA metabarcoding analysis revealed a high efficacy as a screening method to identify both plant ingredients and vegetal traces belonging to insect farming or possible adulteration events, also acting as an early warning strategy for the occurrence of allergens of human concern. This approach could support the development of new risk assessment procedures for novel foods by regulatory authorities to ensure their quality, safety, and acceptance which will become more required in order to face the challenge of feeding the world population in the next decades.
Jessica Frigerio; Giulia Agostinetto; Anna Sandionigi; Valerio Mezzasalma; Nadia Maria Berterame; Maurizio Casiraghi; Massimo Labra; Andrea Galimberti. The hidden ‘plant side’ of insect novel foods: A DNA-based assessment. Food Research International 2019, 128, 108751 .
AMA StyleJessica Frigerio, Giulia Agostinetto, Anna Sandionigi, Valerio Mezzasalma, Nadia Maria Berterame, Maurizio Casiraghi, Massimo Labra, Andrea Galimberti. The hidden ‘plant side’ of insect novel foods: A DNA-based assessment. Food Research International. 2019; 128 ():108751.
Chicago/Turabian StyleJessica Frigerio; Giulia Agostinetto; Anna Sandionigi; Valerio Mezzasalma; Nadia Maria Berterame; Maurizio Casiraghi; Massimo Labra; Andrea Galimberti. 2019. "The hidden ‘plant side’ of insect novel foods: A DNA-based assessment." Food Research International 128, no. : 108751.