Accurate identification of protein-coding regions (exons) in DNA sequences has been a challenging task in bioinformatics. Particularly the coding regions have a 3-base periodicity, which forms the basis of all exon identification methods. Many signal processing tools and techniques have been applied successfully for the identification task but still improvement in this direction is needed. In this paper, we have introduced a new promising model-independent time-frequency filtering technique based on S-transform for accurate identification of the coding regions. The S-transform is a powerful linear time-frequency representation useful for filtering in time-frequency domain. The potential of the proposed technique has been assessed through simulation study and the results obtained have been compared with the existing methods using standard datasets. The comparative study demonstrates that the proposed method outperforms its counterparts in identifying the coding regions.

Understanding the behavioral process of life and disease-causing mechanism, knowledge regarding protein-protein interactions (PPI) is essential. In this paper, a novel hybrid approach combining deep neural network (DNN) and extreme gradient boosting classifier (XGB) is employed for predicting PPI. The hybrid classifier (DNN-XGB) uses a fusion of three sequence-based features, amino acid composition (AAC), conjoint triad composition (CT), and local descriptor (LD) as inputs. The DNN extracts the hidden information through a layer-wise abstraction from the raw features that are passed through the XGB classifier. The 5-fold cross-validation accuracy for intraspecies interactions dataset of Saccharomyces cerevisiae (core subset), Helicobacter pylori, Saccharomyces cerevisiae, and Human are 98.35, 96.19, 97.37, and 99.74 percent respectively. Similarly, accuracies of 98.50 and 97.25 percent are achieved for interspecies interaction dataset of Human- Bacillus Anthracis and Human- Yersinia pestis datasets, respectively. The improved prediction accuracies obtained on the independent test sets and network datasets indicate that the DNN-XGB can be used to predict cross-species interactions. It can also provide new insights into signaling pathway analysis, predicting drug targets, and understanding disease pathogenesis. Improved performance of the proposed method suggests that the hybrid classifier can be used as a useful tool for PPI prediction. The datasets and source codes are available at: https://github.com/SatyajitECE/DNN-XGB-for-PPI-Prediction .

The time-frequency representation (TFR) has been used as a powerful technique to identify, measure and process the time varying nature of signals. In the recent past S-transform gained a lot of interest in time-frequency localization due to its superiority over all the existing identical methods. It produces the progressive resolution of the wavelet transform maintaining a direct link to the Fourier transform. The S-transform has an advantage in that it provides multi resolution analysis while retaining the absolute phase of each frequency component of the signal. But it suffers from poor energy concentration in the time-frequency domain. It gives degradation in time resolution at lower frequency and poor frequency resolution at higher frequency. In this paper we propose a modified Gaussian window which scales with the frequency in a efficient manner to provide improved energy concentration of the S-transform. The potentiality of the proposed method is analyzed using a variety of test signals. The results of the study reveal that the proposed scheme can resolve the time-frequency localization in a better way than the standard S-transform.