The release of oil into the environment represents a pervasive problem stemming from oil exploration, accidents, transportation and releases from landfills, storage facilities or industrial plants. Compared to conventional and costly techniques, bioremediation is an attractive and promising alternative, as it can selectively degrade pollution without affecting the natural features of the site and the flora and fauna living there. In this study, the isolation, identification and characterization of autochthonous bacterial strains from a crude oil-contaminated soil sample was carried out under batch conditions over a period of 28 days. The initial colony forming units value for bacteria was 1.15 × 10⁹/g of soil. The results show that the activity of bacteria was highest at a pH of 7.25. Based on morphology, biochemical tests and an analysis of MALDI TOF, isolated autochthonous bacterial species were identified as Bacillus megaterium, Bacillus licheniformis, Bacillus cereus and Pseudomonas chlororaphis. Since allochthonous bacteria are not highly efficient in a new environment due to their low adaptability and competition with indigenous microorganisms, these autochthonous microorganisms play a pivotal role in enhancing the bioremediation process on an applicable, industrial scale. This advancement aligns with the principles of sustainable development and the green economy, as purified soil is suitable for rational reuse in the environment.