Accurately estimating shrub biomass in arid and semi-arid regions is critical for understanding ecosystem productivity and carbon stocks at both local and global scales. Due to the short and sparse features of shrubs, capturing the shrub biomass accurately by satellite observations is challenging. Previous studies mostly used ground samples and satellite observations to estimate shrub biomass by establishing a direct connection, which was often hindered by the limited number of ground samples and spatial scale mismatch between samples and observations. Unmanned aerial vehicles (UAVs) provide opportunities to obtain more samples that are in line with the aspects of satellite observations (i.e., scale) for regional-scale shrub biomass estimations accurately with low costs. However, few studies have been conducted based on the air-space-ground-scale connection assisted by UAVs. Here we developed a framework for estimating 10 m shrub biomass at a regional scale by integrating ground measurements, UAV, Landsat, and Sentinel-1/2 observations. First, the spatial distribution map of shrublands and non-shrublands was generated in 2023 in the Helan Mountains of Ningxia province, China. This map had an F1 score of 0.92. Subsequently, the UAV-based shrub biomass map was estimated using an empirical model between the biomass and the crown area of shrubs, which was aggregated at a 10 m × 10 m grid to match the spatial resolution of Sentinel-1/2 images. Then, a regional-scale estimation model of shrub biomass was developed with a random forest regression (RFR) approach driven by ground biomass measurements, UAV-based biomass, and the optimal satellite metrics. Finally, the developed model was used to produce the biomass map of shrublands over the study area in 2023. The uncertainty of the resultant biomass map was characterized by the pixel-level standard deviation (SD) using the leave-one-out cross-validation (LOOCV) method. The results suggested that the integration of multi-scale observations from the ground, UAVs, and satellites provided a promising approach to obtaining the regional shrub biomass accurately. Our developed model, which integrates satellite spectral bands and vegetation indices (R² = 0.62), outperformed models driven solely by spectral bands (R² = 0.33) or vegetation indices (R² = 0.55). In addition, our estimated biomass has an average uncertainty of less than 4%, with the lowest values (30%) and biomass production (>300 g/m²). This study provides a methodology to accurately monitor the shrub biomass from satellite images assisted by near-ground UAV observations as well as ground measurements.

Accurately estimating shrub biomass in arid and semi-arid regions is critical for understanding ecosystem productivity and carbon stocks at both local and global scales. However, capturing the shrub biomass accurately by satellite observations is challenging due to the short and sparse features of shrubs. In this study, we presented a framework to estimate shrub biomass at a 10m spatial resolution by integrating ground in-situ data, unmanned aerial vehicle (UAV), Sentinel-2, and Sentinel-1 observations. The pilot study was conducted in the Helan mountains of Ningxia province, China. First, the spatial distribution map of shrubland and other land cover types was generated in 2023. Then, a prediction model of shrub biomass was developed in a Random Forest Regression (RFR) approach driven by different predicted variable datasets based on in-situ measurements, UAV, and satellite images. Finally, the developed model was used to produce the biomass map of shrubland in 2023. The uncertainty was characterized by creating a standard deviation (SD) map using the Leave-One-Out Cross-Validation (LOOCV) method in the shrub biomass estimates. The resultant shrubland distribution map in 2023 has an F1 score of 0.92. The shrub biomass model driven by spectral bands and vegetation indices (R2= 0.62) demonstrated superior performance than that only driven by spectral bands (R2=0.33) or vegetation indices (R2=0.55). The uncertainty of the estimated biomass was lower than 4%, with the lowest values (30%) and biomass production (>30kg/m2) regions. Furthermore, the findings show that the magnitude of shrub biomass was affected by the aridity and precipitation significantly in the study region. This study provides a workflow to accurately monitor the biomass of shrublands in a complicated environment based on remote sensing images from multi-platform observations.