The present study was conducted to evaluate the toxicity induced by the increasing doses of potassium dichromate in rabbit doe. Twenty-eight adult does of 6 months of age were divided into four groups (A, B, C, and D; n = 7), with comparable average body weight (bw). Group A rabbits received only distilled water daily and served as a control, while groups B, C, and D received, respectively, 10 mg/kg bw, 20 mg/ kg bw, and 40 mg/kg bw of potassium dichromate via gavage for 28 days, after which animals were anesthetized with ether vapor and sacrificed. Blood samples were obtained via cardiac puncture and collected without anticoagulant for biochemical dosages and with anticoagulant (EDTA) for complete blood count. Follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) were dosed in serum and in homogenates of ovary with the help of AccuDiag^TM ELISA kits from OMEGA DIAGNOSTICS LTD (Scotland, England) while respecting the immuno-enzymatic method. Activities of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and concentration of malondialdehyde (MDA) in liver, kidney, ovary and uterus were measured. Hematology revealed a significant (p < 0.05) decrease in mean values of hemoglobin and platelets while white blood cells and lymphocytes showed a significant (p < 0.05) increase in exposed groups. No significant (p > 0.05) difference was registered in monocytes, red blood cells, hematocrits, and plaquetocrits values with respect to the control. No matter the organ considered, no significant (p > 0.05) change was recorded in weight and volume. Nephrotoxicity analysis registered a significant (p < 0.05) increase in urea and creatinine, unlike renal tissue protein, which decreased significantly (p < 0.05). However, hepatotoxicity registered no significant (p > 0.05) variation in aspartate aminotransferase but total protein, alanine aminotransferase, and total cholesterol increased significantly (p < 0.05), while hepatic tissue protein revealed a significant (p < 0.05) decrease. Analysis on reproductive parameters showed a significant (p < 0.05) decrease in ovarian and uterine tissue proteins, as well as in follicle stimulating hormone, luteinizing hormone, and estradiol. Oxidative stress markers recorded no significant (p > 0.05) difference in glutathione reductase except in ovary where a significant (p < 0.05) decrease was seen when compared with the control, while catalase revealed a significant (p < 0.05) decrease, except in liver where there was no significant (p > 0.05) change. Superoxide dismutase and malondialdehyde recorded a significant (p < 0.05) decrease and increase respectively, with respect to the control. Results obtained from this study showed that the reduction process of chromium in tissues may cause the generation of reactive oxygen species, which are involved in hematoxic, nephrotoxic, hepatotoxic, and reproductive toxicity effects.

Nephropathies and especially nephrotoxicity have become one of the serious causes of life-threatening conditions because of intensive exposure to xenobiotic whether by environmental pollution or by drug abuse. The present study was undertaken to assess the protective effects of Cinnamomum zeylanicum stem bark aqueous extract (AECZ) on gentamicin-induced nephrotoxicity. AECZ was prepared by maceration in water and tested orally at the doses of 200 and 400mg/kg/day to prevent gentamicin-induced nephropathies in male Wistar rats. Gentamicin (100mg/kg/day) was administered for 14 consecutive days by intraperitoneal route, concomitantly with AECZ or silymarin (50mg/kg/day) used as reference drug. Animal body weight was monitored during the treatment. After the last treatment on the 14th day, animals were sacrificed. Blood was collected for the evaluation of hematological and renal function biomarkers. The homogenate of one kidney was used to assess oxidative stress markers and proinflammatory cytokines, while the other one was fixed in formaldehyde for histopathological studies. Gentamicin decreased body weight, serum total proteins, and calcium level but increased kidneys relative weight, serum creatinine, urea, and uric acid. Moreover, the levels of reduced glutathione, catalase, and superoxide dismutase activities were decreased, while an increase in malondialdehyde, proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and nitrites was observed in the negative control group as compared to normal control. Histological analysis of the kidney revealed the presence of tubular necrosis, glomerular degeneration, and macrophage infiltration in the gentamicin-treated group. All these impairment parameters were prevented by AECZ and silymarin treatments. AECZ has a protective effect against gentamicin-induced nephrotoxicity. The antioxidant and anti-inflammatory potentials of this extract may highly contribute to its nephroprotective activity.

Leishmaniasis causes the ninth largest disease burden among infectious diseases but remains a very neglected tropical disease. Although the disease is endemic in Cameroon and some neighboring countries, data on its epidemiology are very scanty. The present review summarizes the available information on leishmaniasis in the central region of Africa. According to available records, Cameroon, Chad and Nigeria have been identified as endemic foci of both cutaneous (CL) and visceral leishmaniasis (VL). In addition, the phlebotomine vectors of leishmaniasis have been reported in these three countries and also in Congo and the Central African Republic. Although Gabon, Central African Republic, Equatorial Guinea and Congo are all situated next to the above leishmaniasis-endemic countries and are characterized by similar landscapes and vegetation, they lack published reports of autochthonous cases of leishmaniasis. Considering that many cases of the disease might remain unreported, it might not be an overstatement to recommend that research should be carried out in Gabon, Equatorial Guinea, Central African Republic and Congo to identify cases of leishmaniasis (CL and/or VL), the parasite and vector species, and the mammalian reservoir host. This review updates data on leishmaniasis and its insect vector in the geographical region of Central Africa. Such updates are basic requirement for the development of successful control programmes in individual countries and the whole region. In order to address the shortcomings identified in the present review, the authors recommend training of more scientists in leishmaniasis epidemiology in the region that should be accompanied by necessary funding. This training must be multidisciplinary and include development of laboratory and field skills for studies of the parasite, the vector, the reservoir, the vegetation and the soil in potential endemic foci. In addition, prospective studies involving geographers and other experts should develop a disease risk map of the Central Africa region.

Vitex cienkowskii stem-bark is used in Cameroonian traditional medicine to treat cardiovascular diseases including hypertension. In previous studies, the methanol/methylene chloride stem-bark extract of Vitex cienkowskii (MMVC) showed a preventive activity in L-NAME-induced hypertension and improved blood pressure of spontaneously hypertensive rats. The present study investigated the curative effects in L-NAME-induced hypertensive rats (LNHR). Hypertension was induced in rats by oral administration of L-NAME (40mg/kg/day) for 28 days. The animals were divided into 2 groups: one group of 5 rats receiving distilled water (10ml/kg) and another 20 rats receiving L-NAME. At the end of 4 weeks of administration of L-NAME, the animals were divided into 4 groups of 5 rats each: one group of hypertensive rats receiving distilled water, another one receiving captopril (25mg/kg), and two groups of hypertensive rats receiving MMVC at doses of 200 and 400mg/kg, respectively. Body weight, food, and water intake were measured weekly. At the end of the treatment, blood pressure and heart rate were recorded by invasive method. Whole heart, left ventricle, kidneys, and liver were weighed. The effects of plant extract on lipid profile and oxidative stress markers, as well as markers of hepatic and renal functions were assessed spectrophotometrically according to well described protocols. Results show that L-NAME significantly increases the mean arterial blood pressure (MABP), atherogenic index, lipid profile, and creatinine and transaminase activities of normotensive rats. MMVC significantly reduced the blood pressure in LNHR. Body weight, food and water intake, left ventricular hypertrophy, antioxidant level, renal and hepatic markers, and lipid profile were improved by the treatment with MMVC. The curative effect of MMVC on L-NAME-induced hypertension is probably related to its antihypertensive, hypolipidemic, and antioxidant properties. These results confirmed the use of Vitex cienkowskii for the treatment of hypertension in traditional medicine.

Objective. To determine the prevalence rate of HIV and diabetes among tuberculosis (TB) patients and also the comorbidity rate. Design. Cross-sectional study. Setting. This study was carried out at the Tuberculosis Reference Laboratory, Regional Hospital Bamenda, North West Region of Cameroon, from January 2017 to December 2019. Participants. 1115 cases of pulmonary tuberculosis aged ≥14 years (mean 42.5 ± 15.28 years). Methods. Sputum samples collected were acid-fast stained and examined macroscopically as well as inoculated for culture. A chest X-ray was performed for further confirmation of TB diagnosis. After the TB diagnosis was done, fasting blood glucose, 2 h-PG test, HbA1c, and biochemical enzymatic tests were performed for the diagnosis of diabetes. Rapid strip test and enzyme-linked immunosorbent assay were used to diagnose HIV infection. Interventions. No intervention was done during the period of study. Outcome Measures. The prevalence of TB/HIV and TB/HIV/DM, signs and symptoms, imaging results, and bacteriology status among TB/HIV, TB/HIV/DM coinfected, and comorbidity cases. Results. Of 1115 participants, 38.57% had TB/HIV, and 5.83% had TB/HIV/DM. Among TB/HIV/DM cases, 20.39% had a cough for more than 2 weeks [ ; OR (95%CI): 4.866 (3.170–7.404)], and 35.71% had a fever for at least 2 weeks [ ; OR (95%CI): 7.824 (5.336–11.36)]. The majority of TB/HIV/DM patients (77.42%) had chest pain for at least 2 weeks [ ; OR (95%CI): 114.3 (59.78–207.1)]. 7.41%, 14.18%, and 9.09% of TB/HIV/DM, respectively, had chest abnormality, positive smear, and positive culture (p = 0.018). Significant differences were observed between signs and symptoms, imaging results, bacteriology, treatment history for TB cases and those with HIV and/or DM, and those without HIV and/or DM coinfection and comorbidity. Conclusion. This study reports a high prevalence of DM comorbidity and HIV coinfection among active TB patients in the North West Region of Cameroon as well as TB/HIV/DM comorbidity.

Leishmaniasis, an anthropozoonotic infection transmitted to humans and animals through female sandfly bite, is endemic in the far north region of Cameroon, which currently lacks a control programme. This study aimed to assess the occurrence and monthly distribution of sandflies in Kousseri County, a region with reported cases of visceral leishmaniasis (VL), to provide data needed for combatting the disease. Sandfly occurrence was evaluated by monthly deployment of six CDC light traps over a twelve-month period (from September 2020 to August 2021) in urban, peri-urban and rural ecotopes. Our survey collected 4,214 sandflies, including 51.02% females. The highest abundance of sandflies was recorded in the rural areas (43.43%, n = 1830) and in January during the dry season (16.22%, n = 679). Ten sand fly species from the Sergentomyia genus Franca and Parrot 1920 and one species of the Phlebotomus genus Rondani and Berté 1840 were identified. The monthly dynamics of the collected female sandflies varied markedly: Se. antennata Newstead, 1912, the most prevalent species and Se. squamipleuris Newstead, 1912 showed unimodal peaks in January and February, respectively; Se. schwetzi Adler, Theodor & Parrot, 1929 exhibited a trimodal pattern with a major peak in September; Se. distincta followed a bimodal trend with peaks in January and May. The aforementioned sandfly species were present throughout the study period, while other species displayed variable and intermittent presence. Ph. duboscqi, the sole known vector of leishmaniasis in this study, was absent in January and July, but peaked in September, representing only 1.07% of the total catch. The Shannon–Wiener index was high (H > 1) in all the collected ecotopes and months, indicating high evenness and Simpson indices. Overall, the findings suggest that sandflies are active year-round in Kousseri, with elevated abundance during the dry season, particularly in January and March. The presence of animal shelters surrounding houses, vegetation (primarily rice paddies) and building conditions with unplastered, fissured mud walls likely favour a high abundance of sandflies in rural areas in Kousseri. These findings highlight the need of optimising surveillance and intervention strategies, especially in rural settings and during months when sandflies are more active. Further studies on climatic and environmental factors affecting sandfly distribution, as well as investigations into their feeding habits and Leishmania infection rates, are crucial for enhancing disease control in the region.