Improving water use efficiency is essential for the advancement of agricultural production, particularly in arid and semiarid regions. Two-year field experiments were conducted to study the effects of ridge–furrow (RF) and flat planting (FP) plastic film mulching combined with five different nitrogen (N) fertilizers, N1 (KNO₃), the nitrate (NO₃⁻)/ammonium (NH₄⁺) mixtures with different pure nitrogen ratios N2 (1:1), N3 (1:3), and N4 (3:1), and the control N5 (urea) on maize dry matter accumulation, soil water content, grain yield, water use efficiency (WUE), and N partial factor productivity. Our results showed that RF and N4 were more efficient than FP for increasing maize grain yield, WUE, and nitrogen partial factor productivity, and there was a significant interaction for cultivation practices × N formulation. RF and 3:1 NO₃⁻/NH₄⁺ significantly increased grain yield by 14.73% and 13.15%, and 20.07% and 24.14% in 2016 and 2017, respectively, compared to FP and nitrate only. RFN4 produced the highest grain yield in 2016 and 2017 due to the highest dry matter accumulation at filling and physiological maturity stage, ear rows per spike, and row grains per row. Over two growing seasons, the WUE and N partial factor productivity under RFN4 were 18.75% and 29.17% more on average than those of other treatments. Therefore, RFN4 is an effective planting system for increasing the simultaneity of grain yield and WUE for maize production in rain-fed agriculture.

In order to understand the mechanism of action of oil and fatty acid accumulation and desaturase gene expression in how oilseed flax responds to different fertilization conditions, a three-factor split-plot experiment was conducted to investigate the accumulation trends of oil and fatty acids. The results revealed that soluble sugar (SS) and sucrose (SUC) contents decreased, and the starch (ST) content increased gradually with the grain development and maturity of oilseed flax. The application of sheep manure promoted the accumulation of nonstructural carbohydrates in the grains. Soluble sugar (SS) and sucrose (SUC) contents were negatively correlated with the oil content. Compared with chemical fertilizer, organic manure decreased the total saturated fatty acid but increased the unsaturated fatty acid. Organic manure significantly upregulated the expression of various genes, and fad2a gene expression was higher with the 5.8 t ha⁻¹ chicken manure treatment. The 25 t ha⁻¹ sheep manure treatment was more conducive to the expression of fad3a and fad3b genes and promoted the accumulation of linolenic acid (LIN), and the LIN content increased by 0.64–3.90% compared with other treatments. Consequently, an ongoing anthropogenic sheep manure input may impact the regulation of grain oil quality and yield per unit area.

Crop rotation aims to improve the sustainability and production efficiency of agricultural ecosystems, especially as demands for food and energy continue to increase. However, the regulation of soil microbial communities using crop rotation with oilseed flax and its relationship with key soil physicochemical driving factors are still not clear. In order to investigate this matter, we carried out a field study lasting four years involving various crop rotation sequences including FWPF, FPFW, PFWF, FWFP, ContF, and ContF1. In addition to evaluating soil physicochemical parameters, we employed Illumina high-throughput sequencing technology to explore the structure and variety of soil microbial communities. The findings indicated a notable rise in pH value with the FPFW treatment in contrast to other treatments, along with significant increases in AP, MBC, MBN, and qSMBC compared to ContF. The number of OTUs in the FPFW, WFPF, FPFW, and PFWF treatments was significantly increased by 4.10–11.11% compared to ContF (p < 0.05). The presence of Actinobacteria and Acidobacteria was greatly impacted by the FPFW treatment, whereas the presence of Actinobacteria and Chloroflexi was notably influenced by the ContF treatment. The soil bacterial community was primarily influenced by TC, pH, and NO₃⁻-N according to correlation analysis. Specifically, the FPFW therapy notably raised the soil pH level while lowering the TC level. Furthermore, the FPFW therapy led to a notable rise in the proportion of Acidobacteria and a significant decline in the proportion of Actinobacteria. These findings provide important theoretical support for using FPFW rotation to regulate soil microbial communities and solve the problems of continuous cropping.