Vitis vinifera L. subsp. sylvestris (sylvestris) is the only native wild grapevine in Eurasia (Europe and western Asia) and is the existing ancestor of the grapevine varieties (for wine and table grape production) belonging to the subsp. sativa. In Slovenia, the prevailing opinion has been that there are no Slovenian sylvestris habitats. This study describes sylvestris in Slovenia for the first time and aims to present an overview of the locations of the wild grapevine in the country. In this project, a sample set of 89 accessions were examined using 24 SSR and 2 SSR markers plus APT3 markers to determine flower sex. The accessions were found in forests on the left bank of the Sava River in Slovenia, on the border between alluvial soils and limestone and dolomite soils, five different sites, some of which are described for the first time. The proportion of female to male accessions differed between sites. At two sites, female plants dominated; at others, the ratio was balanced. The plants’ genetic diversity and structure were compared with autochthonous and unique varieties of subsp. sativa from old vineyards in Slovenia and with rootstocks escaped from nature from abandoned vineyards. Sylvestris was clearly distinguishable from vinifera and the rootstocks. Based on genetic analyses, it was confirmed that Slovenian sylvestris is closest to the Balkan and German sylvestris groups. Meanwhile, a safety duplication of the wild grapevine accessions has been established at the University Centre of Viticulture and Enology Meranovo, Faculty of Agriculture and Life Sciences at the University of Maribor.

In this study, the development trends of bioclimatic parameters recorded at the Maribor and Murska Sobota climate stations from 1952 to 2022 and the dynamics of grape ripening in early-, medium-, and late-ripening grape varieties in the Podravje wine-growing region in Slovenia (north-eastern Slovenia) from 1980 to 2022 were investigated. Based on the data on soluble solids content, total acidity, and the recommended harvest date per year (until the technological ripeness of the grapes; 76°Oe), trends for shortening the growing period of the vines were calculated. Temperature changes have been more pronounced since 1980. The number of so-called hot days (with a maximum of T > 30 °C) has increased the most, which has the greatest impact on other bioclimatic parameters, e.g., the average temperature and growing degree days (GDDs) and the Huglin index (HI). For the period of 1980 to 2022, the trends were 0.44 °C (Murska Sobota) and 0.51 °C (Maribor) per decade, respectively. The trends were more pronounced for the average temperature in the period of May–June (TMJ). After 1980, the HI increased by about 10 units per year. As a result of the climate warming, grapes in north-eastern Slovenia ripened 26 (‘Sauvignon Blanc’) to 35 (‘Welschriesling’) days earlier. The trends showed a decrease in total acidity, which can be attributed to the higher temperatures during the growing season period, especially during the ripening period of the grapes (véraison). After 2010, the average temperatures during the growing season (1 April to 31 October) in Podravje were 1.6 °C higher than in the 1980s. In line with the earlier ripening of the grapes, the actual average temperature from 1 April to the harvest date was a further 1.0 °C higher. The higher temperatures in the late-ripening varieties ‘Riesling’ and ‘Furmint’ had a positive effect on the lower total acidity. Total annual precipitation and precipitation in the growing season for the period 1980 to 2022 in the Maribor area show decreasing trends of 6 mm/m² (p = 0.001) and 4 mm/m² (p = 0.012), respectively. In the eastern sub-wine-growing region of Podravje (Murska Sobota), the trends in precipitation were not significant.

The long-term use of copper (Cu) fungicides in viticulture in Europe has led to Cu accumulation in vineyard top soils. However, less is known about the accumulation of Cu in grapevine grafts after the callusing process/before planting in the nursery. This paper presents the capacity of 5BB and SO4 rootstocks to accumulate Cu, as well as the patterns of translocation in the grafts. After heat forcing (callusing), the grapevine grafts of Sauvignon Blanc on 5BB and SO4 rootstocks were grown in pots for six months in a glasshouse and exposed to various Cu formulations (Cu-oxychloride, Cu-gluconate) and concentrations in peat (50, 150, 500, and 1000 mg Cu of dry weight (DW)). In addition to monitoring the shoot growth dynamics and analyzing the copper content in graft organs, bioaccumulation (BAFs) and translocation factors (TFs) of Cu were calculated. The mean Cu concentrations were ranked as follows: roots (15–164) > rootstock trunks (8–38) > canes (5–21) mg kg⁻¹ DW. The Cu concentrations depended on the Cu formulation and concentration in the substrate. Higher Cu content was found in the roots of both rootstocks (5BB and SO4, 23–155 and 15–164 mg kg⁻¹ DW, respectively) and the lowest in the canes (less than 10 mg kg⁻¹ DW) of grafts grown in Cu-oxychloride-treated peat. Based on the BAFs and TFs, both rootstocks could be considered as Cu exclusive. A higher translocation rate was determined in systemic Cu-gluconate and SO4 rootstock. With shoot length measurements, the significant inhibitory effects of Cu on grapevine grafts growth could not be confirmed, despite the inhibitory effects that were clearly expressed in the first two months of growth. Soils containing more than 500 mg Cu/kg⁻¹ are less suitable for growing vine grafts.

This study examined the structure and trends of climate parameters important for grape production from 1952 to 2022 in the wine-growing regions of Podravje, Posavje, and Primorska in Slovenia. Average and extreme temperature and precipitation data from six meteorological stations in three wine-growing regions were divided into annual and growing seasons. The results show that in the period 1991–2022, there was a warming in the growing season in all regions by 1.4–1.7 °C, except the southern part of Primorska (Koper station) 0.6 °C, compared to the reference period 1961–1990. The heat accumulation indices (GDDs and HI) increased significantly, which is mainly due to the increase in the maximum temperature in the growing season temperature (GST max) and the number of days with Tmax > 30 °C (NDT30). The NDT30 increased the most, by a factor of more than four. In the reference period (1961–1990), however, the trend in the number of hot days was even slightly negative. The mean seasonal temperature rose to around 17 °C in regions with a continental climate and to around 19 °C in the Mediterranean region, which could be reflected in the earlier ripening of the grapes. The trends show a decrease in total annual precipitation (AP) after 1991, but this was significant only at one inland location (Maribor), while the total precipitation during the growing season (GSP) decreased significantly at three locations (Maribor, Bilje, and Koper).

The aim of this research work was to find historical varieties that existed in this area before phylloxera and to identify them on the basis of historical written sources and genetic analyses. At the beginning of the 19th century, around 300 grape varieties were cultivated in Styria. Between 2020 and 2022, old vineyards were monitored at 115 locations in Styria (between the Mura and Sava rivers) in Slovenia. The directly collected samples (340 grapevine accessions) were determined by molecular analysis with 24 SSR markers. A total of 66 different genotypes were detected. After comparison with the available databases, 29 historical varieties and 37 unknown historical genotypes were identified. Several parameters were calculated to evaluate the usefulness of the selected loci in this work, and a dendrogram representing the genetic similarities between the origins was created using the neighbor-joining method to investigate possible ancestry relationships in the sample set. The most common historical varieties were ‘Belina’ (‘Heunisch weiss’), ‘Vrbovec’ (‘Tantovina Eihenblaetrige’), ‘Ranfol’ (‘Ranfol beli’), and ‘Pelesovna’ (‘Vulpea’). Varieties from the current variety list were also frequently found, such as ‘Frankinja’ (‘Blaufraenkisch’) and ‘Žametovka’ (‘Kavčina črna’). In a few locations, one of the most important red varieties from the beginning of the 20th century was also found in this area (alongside ‘Frankinja’ and ‘Žametovka’), i.e., ‘Vranek’ (‘Zimmettraube balu’). At that time, this variety was planted in multi-variety vineyards and was preserved, but its importance in single-variety vineyards quickly declined due to female flower. In addition, genetic analyses have shown that 37 unknown historical genotypes have been found in this area. These genotypes need to be described ampelographically and technologically evaluated in the future. Most of the vegetative offsprings of these genotypes have already been transferred to the Meranova gene bank, where they can be accurately described ampelographically under the same pedoclimatic conditions.