Fuel burn during the actual route flown is an important indicator of aircraft operational efficiency. This study aims to assess and systematically evaluate the method for fuel consumed during flights using data from the automatic dependent surveillance–broadcast (ADS-B), European reanalysis (ERA5) meteorological dataset, and BADA 3 performance. A literature background and comprehensive methodology are provided for fuel estimation using track data. The airborne part of the trajectory was used to estimate the total trip fuel consumed during several flights of a commercial airliner. The calculated fuel burn is compared with measured fuel consumption from the flight data recorder (FDR). The results show that fuel consumption for the entire airborne part of the trajectory can be estimated with an average error of 1.2% and with a standard deviation of 1.3%. Detailed results of fuel burn for individual flight phases, from the initial climb to the approach, are also presented. In addition, this paper also discusses the sources of errors and the potential applications of the method for network operations and environmental monitoring.