Disturbing the lignocellulose digestive system of termites is considered to be a promising approach for termite control. The research on the tolerance mechanism of the termite lignocellulose digestive system to harmful environment conditions is limited. In this study, we keep Coptotermes formosanus Skiraki under a non-lethal toxic condition by feeding the termites with filter paper containing the kojic acid (a low toxic insecticide). The effects of low toxic stress on the activities and gene expressions of host/symbiotic originated lignocellulases, and on the symbiotic microbial community structure of C. formosanus were explored. Our result showed that the low toxic stress would lead to the synchronous decrease of cellulase and hemicellulase activities, and supplementary increase of corresponding gene expressions. The symbiotic community maintained its role as the main force in the lignocellulolytic system of C. formosanus. Meanwhile, a large number of rare taxa were significantly enriched by kojic acid treatment. These numerically inconspicuous bacterial populations might be responsible for the functions similar to phenoloxidase or insecticide detoxification and enable C. formosanus to tolerate the harmful environment. Overall, our data suggested that the digestive adaptation of C. formosanus to physiotoxic feeding is closely related to the triple collaboration of termites–flagellates–bacteria.

The taxonomy of Reticulitermes Holmgren (Isoptera: Rhinotermitidae) in China is problematic and in need of revision. Most Chinese Reticulitermes species were described in the 1980s and 1990s, and have never been studied since then. In this study, morphological characteristics including coloration, pilosity, shape, and morphometric characteristics of Reticulitermes dichrous Ping and Reticulitermes guangzhouensis Ping were compared. In addition, a portion of the ribosomal RNA large subunit 16S (16S rRNA) and the mitochondrial DNA cytochrome oxidase subunit II (COII) genes from different populations of the 2 species were sequenced and analyzed. Morphological comparisons revealed the similarities between the 2 species in both discrete and morphometric characteristics. In the molecular phylogenetic trees inferred from COII and 16S rRNA genes, all of the examined populations of the 2 species clustered into a common clade with a high bootstrap value. Based on the morphological comparisons and the molecu... Resumen La taxonomía del género Reticulitermes Holmgren (Isoptera: Rhinotermitidae) en China es problemática y necesita revisión. La mayoría de las especies chinas de Reticulitermes fueron descritas en los años ochenta y noventa, y nunca se han estudiado desde entonces. En este estudio se compararon las características morfológicas incluyendo coloración, pilosidad, forma y características morfométricas de Reticulitermes dichrous Ping y Reticulitermes guangzhouensis Ping. Además, se secuenció y analizó una parte de la subunidad 16S (16S rRNA) del ARN ribosómico y los genes de la subunidad II de la citocromo oxidasa (COII) del ADN mitocondrial de diferentes poblaciones de las 2 especies. Las comparaciones morfológicas revelaron las similitudes entre las 2 especies tanto en características discretas como morfométricas. En los árboles filogenéticos moleculares inferidos de los genes COII y 16S rRNA, todas las poblaciones examinadas de las 2 especies se agruparon en un clado común con un alto valor de bootstra...

Cellulose digestion is an essential process of termites, and it is accomplished by three types of cellulases. β-Glucosidase (BG), one of the critical cellulases responsible for cellulose degradation and glucose production, has been considered as a potential target for pest management strategies. Previous experiments identified two new endogenous BG homologs, CfBG-Ia and CfBG-Ib, in the digestive system of Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). The objectives of this study were to assess the impact of RNA interference on CfBG-Ia and CfBG-Ib expression and on termite survival. We tested the expression profiles of worker termites which were injected with gene-specific double-stranded RNA (dsRNA, targeting one gene at a time) and a dsRNA cocktail (targeting CfBG-Ia and CfBG-Ib simultaneously). The expression of CfBG-Ib showed a sharp decline in both dsCfBG-Ib and dsRNA cocktail treatments. The expression of CfBG-Ia reduced quickly and significantly in the dsRNA cocktail treatment; while in dsCfBG-Ia treatment, it decreased on the fifth day. Results showed that treatment with the dsRNA cocktail caused greater inhibition of the transcript expression and a shorter response time. However, the expression of nontarget BG homologs was increased as the target BG homologs were being repressed during the testing period in dsRNA cocktail treatment. These results demonstrate that targeting cellulase-coding genes may be a potential strategy to inhibit termite digestion process, or at least dsRNA cocktails serve as a means for identifying the most susceptible target gene families or biological processes.

The Formosan subterranean termite, Coptotermes formosanus Shiraki, is a serious pest worldwide. Juvenile hormone analog (and its analogs such as methoprene) can induce the transformation of the worker caste into soldier caste in C. formosanus. However, several factors, such as feeding substrate and colony origin, influence the proportion of solider formation. The molecular mechanism of worker to soldier transformation of C. formosanus is still not clear. RT-qPCR is a powerful tool for molecular studies. Accurate gene quantification by the relative quantification method requires a stable expressed gene as the reference gene. However, no reference genes were available for this species in the methoprene bioassay. To study the problem of gene response to methoprene by RT-qPCR we have to first screen reference genes in C. formosanus. Workers were fed with methoprene. Termites were collected during the methoprene bioassay and separated into head and thorax+abdomen. Expression profiles of 10 candidate reference genes in the two body part types were investigated using RT-qPCR. The results were analyzed by a set of established methods (geNorm, NormFinder, BestKeeper, and RefFinder) as well as comparative ΔCt method. Our results suggest that RPS18 is the most stably-expressed gene both in the head and thorax+abdomen for expression analysis in the methoprene bioassay of C. formosanus. The screening of suitable reference genes in C. formosanus establishes the foundation for the molecular study of soldier caste differentiation in this species.

Orphaned colonies of Cryptotermes domesticus readily produce replacement reproductives and continue propagation. In this study, we aimed to investigate the production and fecundity of neotenic reproductives in 5-year-old colonies of C. domesticus after orphaning. All 15 experimental colonies were successfully re-established by the neotenic reproductive pair. Three types of neotenic reproductives with various wing-bud lengths were observed: type I with micro wing buds, type II with short wing buds, and type III with long wing buds. Four patterns of pairs made up of these neotenics, namely, type I + type II, type I + type III, type II + type II, and type II + type III, exhibited reproductive capacities similar to those of the primary reproductive pair. We speculated that these neotenic reproductives were derived from various nymphal instars. The 5-year-old colonies had three instars of nymphs, with the majority being in the second instar, followed by the first. Thus, the combination of neotenic reproductives with short wing buds and micro wing buds was the dominant differentiation pathway of the orphaned colonies. After the removal of the original primary reproductive pair, the nymphs matured into neotenic reproductives and took over reproduction in the colony in 107.40 ± 15.18 days. This study highlights the importance of quarantine and routine inspection of wood, as well as the significance of early prevention and control of C. domesticus infestation in wood. Moreover, this study confirms the high differentiation and reproductive capacities of C. domesticus.