To improve the self-healing efficiency of bacterial concrete, one type of bacteria which could survive, germinate and induce calcium carbonate precipitation at 7 °C was investigated and incorporated into concrete directly. The results showed that after healing for 14 d, cracks with 400–500 μ m width could be sealed, and higher water resistance was obtained for cracked specimens with bacteria, compared with plain cracked concrete. Therefore, it could be concluded that the bacteria has the potential of promoting concrete self-healed at low temperature.