Millisecond pulsars (MSPs) are known for their long-term stability. Using six years of observations from the Neutron Star Interior Composition Explorer (NICER), we have conducted an in-depth analysis of the X-ray timing results for six MSPs: PSRs B1937+21, B1821-24, J0437-4715, J0030+0451, J0218+4232, and J2124-3358. The timing stability parameter ${\sigma }_z$ has been calculated, revealing remarkable timing precision on the order of ${10}^{-14}$ for PSRs B1937+21 and J0437-4715, and ${10}^{-13}$ for PSRs B1821-24, J0218+4232, and J0030+0451 over a timescale of 1000 days. These findings underscore the feasibility of autonomous in-orbit timekeeping using X-ray observations of MSPs. In addition, the consistency of long-term spin-down noise in the X-ray and radio bands has been investigated by comparison with IPTA radio data.