Neuromodulation: Cranial electrode array biocompatibility, Sacral nerve stimulation for bladder malfunction. Cell Biology: Proficient in primary isolation, culture, stem cell induction, and gene transfection. Cell Micro-Encapsulation: Developed encapsulation protocols using a self-constructed device. Flow Cytometry: Established protocols for DNA double-strand break detection. Gene Cloning: Skilled in synthesizing mammalian expression constructs and CRISPR gene editing. Microsurgical Techniques: Efficient in liver transplantation, vascular surgery, and intra-uterine surgeries on rats. Molecular Biology: Experienced in Western blotting, immunoprecipitation, and other foundational techniques. Nano-Encapsulation: Created nanoparticles for cancer treatment and antioxidant therapies in spinal cord injury. Patch-Clamping: Investigated trans-gap-junctional signaling in Caenorhabditis elegans.