Global and local land-cover mapping products provide important data on land surface. However, the accuracy of land-cover products is the key issue for their further scientific application. There has been neglect of the relationship between inclusion probability and spatial heterogeneity in traditional spatially balanced sampling. The aim of this paper was to propose an improved spatially balanced sampling method using landscape pattern-based inclusion probability. Compared with other global land-cover datasets, Globeland30 has the advantages of high resolution and high classification accuracy. A two-stage stratified spatially balanced sampling scheme was designed and applied to the regional validation of GlobeLand30 in China. In this paper, the whole area was divided into three parts: the Tibetan Plateau region, the Northwest China region, and the East China region. The results show that 7242 sample points were selected, and the overall accuracy of GlobeLand30-2010 in China was found to be 80.46%, which is close to the third-party assessment accuracy of GlobeLand30. This method improves the representativeness of samples, reduces the classification error of remote sensing, and provides better guidance for biodiversity and sustainable development of environment.