BRAF mutation in colorectal carcinomas with signet ring cell component

In this study, polyhydroxybutyrate (PHB) coated magnetic nanoparticles were prepared which are targetable to tumor cells in the presence of a magnetic field. The structural properties, functional groups, size distribution, and magnetic properties of the synthesized PHB coated magnetic nanoparticles were characterized by X-ray diffraction, Fourier transform infrared spectrometer, transmission electron microscopy, dynamic light scattering, vibrating sample magnetometry, and thermogravimetric analysis. PHB coated nanoparticles are efficiently internalized by the cancer cells in culture, without cytotoxicity. The results demonstrated that PHB coated iron oxide nanoparticles are suitable for targeted delivery of drugs such as chemotherapeutics, siRNA, miRNA, or antibodies.

In this study, MNPs were synthesized, coated with biocompatible polyethylene glycol (PEG) and conjugated with folic acid. Crystal and chemical structures, shape, size and magnetic properties of synthesized nanoparticles were characterized. Agglomeration tendency of naked nanoparticles were prevented by oleic acid addition during the synthesis. All synthesized MNPs have been found to exhibit superparamagnetic behavior at 23°C and 37°C. Cytotoxic effects of MNPs were investigated on Doxorubicin, Zoledronic acid and Paclitaxel resistant MCF-7 breast cancer cell lines. The synthesized nanoparticles have been found to be suitable for drug targeting in terms of size, shape, magnetic and cytotoxic properties.