In order to control the amount of greenhouse gas emissions from agriculture, it is necessary to select the appropriate crop rotation and tillage intensity depending on the soil type and climatic conditions. However, their implementation in agricultural management methods has been insufficient until now. The main objective of this study was to investigate the changes and dependences in soil net CO₂ exchange rate (NCER) and main physico-chemical parameters under different tillage (conventional (CT) and no tillage (NT)) and crop rotation systems. Cropping systems significantly affected the amount of nutrients, but did not affect pH and organic carbon; otherwise, tillage vs. cropping systems had no significant effects on the soil chemical parameters analyzed. The data revealed that in NT treatments, the NCER was 28% higher than in CT. Different crop rotations also revealed a significant effect on NCER from the soil. Carbon dioxide fluxes increased in cropping systems where a higher share of catch crops were included. In NT systems, a comparatively higher soil moisture content was registered. In addition, the rotations with catch crops produced a higher (by 1–3%) soil moisture content. The temperature of the soil surface was not significantly affected by tillage or cropping systems; nevertheless, a trend towards higher soil surface temperatures in CT was determined, which might be affected by enhanced air circulation in the pores. Soil NCER increase correlated negatively with higher soil surface moisture content, while its relationship was positive with soil surface temperature increase. In general, soil surface temperature and moisture were the most significant factors in explaining the fluctuation in NCER from Cambisols in Lithuania under moderate climatic conditions.

Conservation soil management strategies, particularly no-tillage and cropping strategies, have become an effective and widely adopted practice that has a direct influence on root parameters and mitigation of greenhouse gasses. However, the effect of different tillage and cropping strategies on root growth in field conditions is rarely studied. The study aimed to quantify and characterise the relationship between root network development and CO₂ emission and how these parameters are affected by different cropping and tillage strategies. Five different crop rotations were tested, with or without the inclusion of catch crops (CC), by growing them in the soil where different tillage practices were applied. Selected cropping strategies differed among themselves in terms of the frequency of CC grown per rotation. The data revealed that in NT treatments, the CO₂ emission (both autotrophic and heterotrophic simultaneously) was 25% higher than in CT. The cropping strategies were identified as an important factor influencing CO₂ emissions. An increase in CO₂ emission varied between 30 and 35% depending on the share of legume and CC inclusion. The frequency of CC grown per rotation has had an effect on the rate of CO₂ emission. The cropping strategy when CC was grown every year showed the lowest amount of CO₂ (by 26%), while in other cropping strategies, when CC was grown once or twice per rotation, significantly higher CO₂ emissions were observed. Root growth and their development were significantly affected (p < 0.05) by soil depth and cropping strategies concerning root length and root volume changes. The inclusion of CC into the rotations led towards a decrease in root volume (by 21%). Root length (R² = 0.45; p < 0.05) and root volume (R² = 0.82; p < 0.05) had a significant impact on soil CO₂ emissions. The results collected from 2021 to 2023 experiments indicated that cropping strategies and CC management areas are important tools not only for the improvement of root parameters but also for understanding how they affect CO₂ emissions. The main message for stakeholders is that the cropping strategies diversification with the inclusion of CC every year in a winter oil-seed rape, spring wheat and pea crop rotation (R/W/P + CC) had demonstrated the possibilities to reduce CO₂ emission and improve the root network parameters as compared to the monoculture strategy.