Epimedium (EM), also known as barrenwort, is a traditional medicinal plant rich in isopentenyl flavonols, which have beneficial biological activities and can improve human and animal health, but its mechanism is still unclear. In this study, ultra-high-performance liquid chromatography/quadrupole-time-of-flight-mass spectrometry (UHPLC-Q-TOF/MS) and ultra-high-performance liquid chromatography triple-quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) were used to analyse the main components of EM, and isopentenyl flavonols such as Epimedin A, B, and C as well as Icariin were the major components of EM. Meanwhile, broilers were selected as model animals to illuminate the mechanism of Epimedium isopentenyl flavonols (EMIE) on gut health. The results showed that supplementation with 200 mg/kg EM improved the immune response, increased cecum short-chain fatty acids (SCFAs) and lactate concentrations, and improved nutrient digestibility in broilers. In addition, 16S rRNA sequencing showed that EMIE altered the composition of cecal microbiome, increasing the relative abundance of beneficial bacteria (Candidatus Soleaferrea and Lachbospiraceae NC2004 group and Butyricioccus) and reducing that of harmful bacteria (UBA1819, Negativibacillus, and Eisenbergiella). Metabolomic analysis identified 48 differential metabolites, of which Erosnin and Tyrosyl-Tryptophan were identified as core biomarkers. Erosnin and tyrosyl-tryptophan are potential biomarkers to evaluate the effects of EMIE. This shows that EMIE may regulate the cecum microbiota through Butyricicoccus, with changes in the relative abundance of the genera Eisenbergiella and Un. Peptostreptococcaceae affecting the serum metabolite levels of the host. EMIE is an excellent health product, and dietary isopentenyl flavonols, as bioactive components, can improve health by altering the microbiota structure and the plasma metabolite profiles. This study provides the scientific basis for the future application of EM in diets.

This study aimed to evaluate the effects of different grinding sieve openings (SO), conditioning temperatures (CTP), and conditioning times (CTM) on feed processing performance, growth performance, meat quality, and serum biochemical indices of broiler chickens fed a diet in which 50 % of corn was replaced with aged paddy rice. A total of 960 white-feathered broiler chickens were randomly divided into 8 groups, with the experiment designed to test 2 SO sizes (2.0 mm and 2.5 mm), 2 CTPs (75 °C and 85 °C), and 2 CTMs (90 s and 180 s). The results showed that the 2.0 mm SO and 180 s CTM significantly improved pellet hardness and the pellet durability index (PDI) (P < 0.05). During the early stages of the experiment, when the broilers digestive systems were not fully developed, a 2.5 mm SO significantly reduced the feed conversion ratio (FCR) (P < 0.001), while 75 °C CTP significantly increased average daily gain (ADG) and body weight (BW) at day 21 (P < 0.05). Additionally, 75 °C CTP significantly reduced the cecum organ weight ratio (P < 0.05). In terms of meat quality, a 2.5 mm SO significantly reduced drip loss and cooking loss at 48 h and 72 h (P < 0.01, P < 0.01, P < 0.05), while a 90 s CTM significantly reduced drip loss and cooking loss at 24 h (P < 0.05, P < 0.01). Furthermore, the combination of 2.5 mm SO and 90 s CTM significantly increased serum urea nitrogen (BUN) levels (P < 0.05). Considering the optimization of both growth performance and meat quality, the combination of 2.5 mm SO, 75 °C CTP, and 90 s CTM is recommended for optimizing aged paddy rice feed processing and enhancing broiler growth performance and meat quality.

Background: The limited bioavailability of free phytosterols restricts their clinical application in managing hypercholesterolemia. This study aimed to develop phytosterol nanoparticles (PNs) to enhance bioactivity and investigate their cholesterol-lowering efficacy and underlying mechanisms in vivo. Methods: Phytosterol nanoparticles (PNs) (93.35 nm) were engineered using soy protein isolate and administered orally at concentrations of 4.00–12.50 mg/mL to high-fat-diet-induced hypercholesterolemic mice (n = 60) over a 4-week period. Serum and hepatic lipid profiles, histopathology, gene/protein expression related to cholesterol metabolism, and fecal sterol content were evaluated. Results: PNs dose-dependently reduced serum total cholesterol (TC: 28.6–36.8%), triglycerides (TG: 22.4–30.1%), and LDL-C (31.2–39.5%), while increasing HDL-C by 18.7–23.4% compared to hyperlipidemic controls (p < 0.01). Hepatic TC and TG accumulation decreased by 34.2% and 41.7%, respectively, at the highest dose, with histopathology confirming attenuated fatty degeneration. Mechanistically, PNs simultaneously suppressed cholesterol synthesis through downregulating HMGCR (3.2-fold) and SREBP2 (2.8-fold), while enhancing cholesterol catabolism via CYP7A1 upregulation (2.1-fold) at protein level. Although less potent than simvastatin (p < 0.05), the nanoparticles exhibited unique dual-pathway modulation absent in conventional phytosterol formulations. Fecal analysis revealed dose-responsive cholesterol excretion (36.01 vs. 11.79 mg/g in controls), indicating enhanced enteric elimination. While slightly less potent than simvastatin (p < 0.05), PNs offered unique dual-pathway modulation absent in conventional phytosterol formulations. Conclusions: Nano-encapsulation significantly improves the bioavailability and hypocholesterolemic efficacy of phytosterols. PNs represent a promising nutraceutical strategy for cholesterol management by concurrently regulating cholesterol synthesis and catabolism, with potential application in both preventive and therapeutic contexts.

To evaluate the effects of dietary threonine levels on rice field eels (Monopterus albus), six experimental diets were formulated with graded threonine supplementation: 0 g/kg (T1), 3 g/kg (T2), 6 g/kg (T3), 9 g/kg (T4), 12 g/kg (T5), and 15 g/kg (T6). Rice field eels, with an initial body weight of 18.47 ± 0.11 g, were fed for 60 days. The weight gain ratio (WGR), specific growth rate (SGR), and protein efficiency ratio (PER) significantly increased in the T4 group, while feed conversion ratio (FCR) significantly decreased. Broken-line regression analysis determined the optimal dietary threonine requirement to be 7.5–9.0 g/kg. Concomitantly, whole-body crude protein content increased, while lipid content decreased; serum GOT and GPT activities declined, indicating reduced hepatic stress, whereas hepatic CAT and SOD activities markedly increased, enhancing antioxidant capacity. Digestive enzyme capacity was enhanced, as evidenced by elevated lipase and trypsin activities. Muscle texture properties, including cohesiveness, springiness, and chewiness, were significantly improved in the T4 and T5 groups. At the molecular level, MyoG, MyoD1, and MYF5 expression peaked in T4, while MRF4 expression increased progressively. LEfSe analysis of the microbiome, coupled with KEGG functional prediction, revealed that Corynebacterium and Methylocella were positively correlated with growth metrics; these genera likely promote muscle deposition through three enriched metabolic pathways that enhance energy and amino acid availability. Collectively, dietary threonine supplementation at 7.5–9.0 g/kg promotes growth, enhances digestive function, and improves muscle quality in rice field eels.