Taenia crassiceps is a zoonotic tapeworm of the genus Taenia that is distributed throughout the Northern Hemisphere. Wild and domestic carnivores are final hosts, while rodents and rabbits are primarily intermediate hosts, although many other mammals may harbour the larval stage, Cysticercus longicollis. This case report aims to describe C. longicollis infection in a lemur and molecularly characterise the isolated parasite. The excised lesion was subjected to morphological and histopathological examination, which revealed cysticerci of the tapeworm. Formalin-fixed and paraffin-embedded block (FFPEB), as well as the cysticerci fixed with formalin stored for one year, were subjected to molecular analysis, which aimed at detecting the partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of Taenia sp. Based on the morphological characteristics, the parasite was identified as a metacestode of T. crassiceps. The presence of the cox1 gene was detected using polymerase chain reaction (PCR) in all samples. A randomly selected PCR product was sequenced and compared with other sequences from the GenBank database, confirming that the detected parasite was T. crassiceps. This article reports the first case of T. crassiceps cysticercosis in a lemur (Lemur catta) in Croatia and emphasises the potential risk of transmission from wild carnivores.

Liver fluke infections are recognised as diseases with worldwide distribution and considerable veterinary and public health importance. The giant liver fluke, Fascioloides magna, is an important non-native parasite which has been introduced to Europe, posing a threat to the survival of local wildlife populations such as red deer (Cervus elaphus). The aim of the study was to analyse differences in liver proteomes between F. magna-infected and control red deer groups using a label-based high-throughput quantitative proteomics approach. The proteomics analysis identified 234 proteins with differential abundance between the control and infected groups. Our findings showed that F. magna infection in this definitive host is associated with changes in the metabolism of proteins and fatty acids, oxidative stress, fibrosis, and signaling pathways. The identified proteins and associated biological pathways represent a valuable contribution to the understanding of host–parasite interactions and the pathogenesis of liver fluke infection.

Toxoplasmosis is a food- and waterborne zoonosis of a great importance, ranked as the fourth most important foodborne parasitosis in the world. The objective of this study was to determine the seroprevalence and molecular prevalence of Toxoplasma gondii in wild boar (Sus scrofa) and to identify genotypes circulating in Croatia. A total of 103 wild boars from four hunting areas in Croatia were screened. Cardiac fluid samples were tested for anti-T. gondii antibodies by MAT, while heart samples were tested for T. gondii DNA using qPCR. The seroprevalence in the cardiac fluid reached 54.4%, while 19.4% of the heart samples were positive by qPCR. The highest seroprevalence was detected in the hunting ground Visocica with 95.6%. Partial microsatellite genotyping was achieved for 2/20 qPCR-positive heart samples suggesting type II strain of T. gondii. Relatively high seroprevalence and detection of T. gondii DNA in tested samples highlights the risk of human infection through consumption of undercooked meat. Further studies focusing on parasite detection in wild boar meat, especially across different climates, are needed to understand regional differences. Expanding research to other wildlife species is also important to clarify their role in the spread of T. gondii.