The McMurdo Dry Valleys are a polar desert in coastal Antarctica, where glaciers, permafrost, ice-covered lakes, and ephemeral summer streams coexist. Liquid water is found at the surface only in lakes and in the temporary streams that feed them. Past geophysical exploration has yielded ambiguous results regarding the presence of subsurface water. In 2011, we used a helicopter-borne, time-domain electromagnetic (TDEM) sensor to map resistivity in the subsurface across the Dry Valleys. The airborne electromagnetic (AEM) method excels at finding subsurface liquid water in polar deserts, where water remains liquid under cold conditions if it is sufficiently saline, and therefore electrically conductive. Over the course of 26 h of helicopter time, we covered large portions of the Dry Valleys and vastly increased our geophysical understanding of the subsurface, particularly with respect to water. Our data show extensive subsurface low-resistivity layers approximately 150–250 m below the surface and beneath higher resistivity layers. We interpret the low-resistivity layers as geologic materials containing freeze-concentrated or “cryoconcentrated” hyper saline brines lying beneath glaciers and frozen permafrost. These brines appeared to be contiguous with surface lakes, subglacial regions, and the Ross Sea, which could indicate a regional-hydrogeologic system, wherein solutes might be transported between surface reservoirs by ionic diffusion and subsurface flow. The presence of such brines underneath glaciers might have implications for glacier movement. Systems such as this, where brines exist beneath glacial ice and frozen permafrost, may exist elsewhere in coastal Antarctica; AEM resistivity is an ideal tool to find and survey them. Our application of TDEM demonstrates that in polar subsurface environments containing conductive brines, such a diffusive electromagnetic method is superior to radar surveying in terms of depth of penetration and ability to differentiate hydrogeologic conditions.

The anoxic and freezing brine that permeates Lake Vida's perennial ice below 16 m contains an abundance of very small (≤0.2-μm) particles mixed with a less abundant population of microbial cells ranging from >0.2 to 1.5 μm in length. Fluorescent DNA staining, electron microscopy (EM) observations, elemental analysis, and extraction of high-molecular-weight genomic DNA indicated that a significant portion of these ultrasmall particles are cells. A continuous electron-dense layer surrounding a less electron-dense region was observed by EM, indicating the presence of a biological membrane surrounding a cytoplasm. The ultrasmall cells are 0.192 ± 0.065 μm, with morphology characteristic of coccoid and diplococcic bacterial cells, often surrounded by iron-rich capsular structures. EM observations also detected the presence of smaller unidentified nanoparticles of 0.020 to 0.140 μm among the brine cells. A 16S rRNA gene clone library from the brine 0.1- to 0.2-μm-size fraction revealed a relatively low-diversity assemblage of Bacteria sequences distinct from the previously reported >0.2-μm-cell-size Lake Vida brine assemblage. The brine 0.1- to 0.2-μm-size fraction was dominated by the Proteobacteria -affiliated genera Herbaspirillum , Pseudoalteromonas , and Marinobacter . Cultivation efforts of the 0.1- to 0.2-μm-size fraction led to the isolation of Actinobacteria -affiliated genera Microbacterium and Kocuria . Based on phylogenetic relatedness and microscopic observations, we hypothesize that the ultrasmall cells in Lake Vida brine are ultramicrocells that are likely in a reduced size state as a result of environmental stress or life cycle-related conditions.

The Southern Ocean receives limited liquid surface water input from the Antarctic continent. It has been speculated, however, that significant liquid water may flow from beneath the Antarctic Ice Sheet, and that this subglacial flow carries that water along with dissolved nutrients to the coast. The delivery of solutes, particularly limiting nutrients like bioavailable iron, to the Southern Ocean may contribute to ecosystem processes including primary productivity. Using a helicopter-borne time domain electromagnetic survey along the coastal margins of the McMurdo Dry Valleys region of Southern Victoria Land, Antarctica, we detected subsurface connections between inland lakes, aquifers, and subglacial waters. These waters, which appear as electrically conductive anomalies, are saline and may contain high concentrations of biologically important ions, including iron and silica. Local hydraulic gradients may drive these waters to the coast, where we postulate they emerge as submarine groundwater discharge. This high latitude groundwater system, imaged regionally in the McMurdo Dry Valleys, may be representative of a broader system of Antarctic submarine groundwater discharge that fertilizes the Southern Ocean. In total, it has the potential to deliver tens of gigagrams of bioavailable Fe and Si to the coastal zone.

Summary: Concurrent osmotic and chaotropic stress make MgCl ₂ ‐rich brines extremely inhospitable environments. Understanding the limits of life in these brines is essential to the search for extraterrestrial life on contemporary and relict ocean worlds, like Mars, which could host similar environments. We sequenced environmental 16S rRNA genes and quantified microbial activity across a broad range of salinity and chaotropicity at a Mars‐analogue salt harvesting facility in Southern California, where seawater is evaporated in a series of ponds ranging from kosmotropic NaCl brines to highly chaotropic MgCl ₂ brines. Within NaCl brines, we observed a proliferation of specialized halophilic Euryarchaeota, which corresponded closely with the dominant taxa found in salterns around the world. These communities were characterized by very slow growth rates and high biomass accumulation. As salinity and chaotropicity increased, we found that the MgCl ₂ ‐rich brines eventually exceeded the limits of microbial activity. We found evidence that exogenous genetic material is preserved in these chaotropic brines, producing an unexpected increase in diversity in the presumably sterile MgCl ₂ ‐saturated brines. Because of their high potential for biomarker preservation, chaotropic brines could therefore serve as repositories of genetic biomarkers from nearby environments (both on Earth and beyond) making them prime targets for future life‐detection missions.

Sulfide inhibits oxygenic photosynthesis by blocking electron transfer between H₂O and the oxygen-evolving complex in the D1 protein of Photosystem II. The ability of cyanobacteria to counter this effect has implications for understanding the productivity of benthic microbial mats in sulfidic environments throughout Earth history. In Lake Fryxell, Antarctica, the benthic, filamentous cyanobacterium Phormidium pseudopriestleyi creates a 1–2 mm thick layer of 50 µmol L⁻¹ O₂ in otherwise sulfidic water, demonstrating that it sustains oxygenic photosynthesis in the presence of sulfide. A metagenome-assembled genome of P. pseudopriestleyi indicates a genetic capacity for oxygenic photosynthesis, including multiple copies of psbA (encoding the D1 protein of Photosystem II), and anoxygenic photosynthesis with a copy of sqr (encoding the sulfide quinone reductase protein that oxidizes sulfide). The genomic content of P. pseudopriestleyi is consistent with sulfide tolerance mechanisms including increasing psbA expression or directly oxidizing sulfide with sulfide quinone reductase. However, the ability of the organism to reduce Photosystem I via sulfide quinone reductase while Photosystem II is sulfide-inhibited, thereby performing anoxygenic photosynthesis in the presence of sulfide, has yet to be demonstrated.