Variable Speed Limit (VSL) systems aimed at reducing congestion and improving safety performance have been implemented around the world in previous years. However, field studies have shown controversial results regarding traffic performance improvement. This study integrates statistical testing methods and clustering techniques for assessing the effect of a non-mandatory VSL system on traffic flow performances on a 14-km portion of the Padua–Mestre motorway in Italy. Statistical analysis is conducted on the observed speeds, collected for almost one year, to identify any significant differences provided by VSL activation. The changes in global motorway performances induced by the VSL in typical traffic patterns under recurring congestion are assessed using both statistical tests and two specific clustering algorithms, namely K-means and DBSCAN. The results indicate that the VSL system effectively affects the observed speeds and alleviates congested conditions: the observed reduction in mean travel time ranges is around 4% with the VSL system active across various lanes; the standard deviation of vehicular speeds witnessed a decrease of 12% to 20% in the most congested segments, while no notable distinction is observed in traffic flows.