Shrimp fry counting is an important task for biomass estimation in aquaculture. Accurate counting of the number of shrimp fry in tanks can not only assess the production of mature shrimp but also assess the density of shrimp fry in the tanks, which is very helpful for the subsequent growth status, transportation management, and yield assessment. However, traditional manual counting methods are often inefficient and prone to counting errors; a more efficient and accurate method for shrimp fry counting is urgently needed. In this paper, we first collected and labeled the images of shrimp fry in breeding tanks according to the constructed experimental environment and generated corresponding density maps using the Gaussian kernel function. Then, we proposed a multi-scale attention fusion-based shrimp fry counting network called the SFCNet. Experiments showed that our proposed SFCNet model reached the optimal performance in terms of shrimp fry counting compared to CNN-based baseline counting models, with MAEs and RMSEs of 3.96 and 4.682, respectively. This approach was able to effectively calculate the number of shrimp fry and provided a better solution for accurately calculating the number of shrimp fry.

Functional peptides have the potential to treat a variety of diseases. Their good therapeutic efficacy and low toxicity make them ideal therapeutic agents. Artificial intelligence-based computational strategies can help quickly identify new functional peptides from collections of protein sequences and discover their different functions.Using protein language model-based embeddings (ESM-2), we developed a tool called pLMFPPred (Protein Language Model-based Functional Peptide Predictor) for predicting functional peptides and identifying toxic peptides. We also introduced SMOTE-TOMEK data synthesis sampling and Shapley value-based feature selection techniques to relieve data imbalance issues and reduce computational costs. On a validated independent test set, pLMFPPred achieved accuracy, Area under the curve - Receiver Operating Characteristics, and F1-Score values of 0.974, 0.99, and 0.974, respectively. Comparative experiments show that pLMFPPred outperforms current methods for predicting functional peptides.The experimental results suggest that the proposed method (pLMFPPred) can provide better performance in terms of Accuracy, Area under the curve - Receiver Operating Characteristics, and F1-Score than existing methods. pLMFPPred has achieved good performance in predicting functional peptides and represents a new computational method for predicting functional peptides.

Addressing the technological requirement for real-time monitoring of fish density in dim aquaculture environments, this study proposes a near-infrared (NIR) image classification method using a modified ResNet18 architecture. Initially, an NIR-Fish dataset comprising 736 high-quality annotated images (256 × 256 resolution) spanning three density scenarios (low, medium, and high density) was constructed. Contrast-Limited Adaptive Histogram Equalization (CLAHE) preprocessing was implemented with an 8 × 8 tiling strategy and clip limit = 4.0, significantly enhancing the discernibility of faint boundary features. A dual-channel attention module (DCAM) was embedded into the ResNet18 backbone, featuring a parallel architecture integrating Global Average Pooling (GAP) and Global Max Pooling (GMP). This design synergistically optimized local salient feature enhancement and global statistical feature fusion through parameter-shared fully connected layers (reduction ratio of 16:1). The experiments show that the classification accuracy of the proposed method on the independent test set is 80.57%, which is 4.34 percentage points higher than that of the original ResNet18. F1 scores for the three density levels were 0.8308 (low), 0.7674 (medium), and 0.8294 (high), respectively. Ablation studies confirmed the dual-channel design’s significant performance contribution, while the parameter-sharing mechanism effectively mitigated overfitting risks. By leveraging feature complementarity and lightweight design, this work overcomes the classification bottleneck for NIR images under low signal-to-noise conditions, providing a highly robust technical solution for intelligent aquaculture management.