Summary: Pentachlorophenol ( PCP ) is globally dispersed and contamination of soil with this biocide adversely affects its functional biodiversity, particularly of fungi – key colonizers. Their functional role as a community is poorly understood, although a few pathways have been already elucidated in pure cultures. This constitutes here our main challenge – elucidate how fungi influence the pollutant mitigation processes in forest soils. Circumstantial evidence exists that cork oak forests in N . W . T unisia – economically critical managed forests are likely to be contaminated with PCP , but the scientific evidence has previously been lacking. Our data illustrate significant forest contamination through the detection of undefined active sources of PCP . By solving the taxonomic diversity and the PCP ‐derived metabolomes of both the cultivable fungi and the fungal community, we demonstrate here that most strains (predominantly penicillia) participate in the pollutant biotic degradation. They form an array of degradation intermediates and by‐products, including several hydroquinone, resorcinol and catechol derivatives, either chlorinated or not. The degradation pathway of the fungal community includes uncharacterized derivatives, e.g. tetrachloroguaiacol isomers. Our study highlights fungi key role in the mineralization and short lifetime of PCP in forest soils and provide novel tools to monitor its degradation in other fungi dominated food webs.

A collective view of the degradation of monochlorocatechols in fungi is yet to be attained, though these compounds are recognised as key degradation intermediates of numerous chlorinated aromatic hydrocarbons, including monochlorophenols. In the present contribution we have analysed the degradation pathways of monochlorophenols in Aspergillus nidulans using essentially metabolomics. Degradation intermediates herein identified included those commonly reported (e.g. 3-chloro-cis,cis-muconate) but also compounds never reported before in fungi revealing for 4-chlorocatechol and for 3-chlorocatechol unknown degradation paths yielding 3-chlorodienelactone and catechol, respectively. A different 3-chlorocatechol degradation path led to accumulation of 2-chloromuconates (a potential dead-end), notwithstanding preliminary evidence of chloromuconolactones and protoanemonin simultaneous formation. In addition, some transformation intermediates, of which sulfate conjugates of mono-chlorophenols/chlorocatechols were the most common, were also identified. This study provides critical information for understanding the role of fungi in the degradation of chlorinated aromatic hydrocarbons; furthering their utility in the development of innovative bioremediation strategies.

SUMMARY: Molecular detection ofBabesiaspecies in apparently healthy cattle within an endemic region was carried out in order to determine the prevalence of carriers and the geographical distribution ofBabesia bigeminaandBabesia bovisin Maputo Province, Mozambique. Samples from 477 animals at 5 localities were analysed using 2 techniques, the semi-nested hot-start PCR and the reverse line blot (RLB) assay. With the semi-nested hot-start PCR, detection ofB. bigeminaranged between 30% and 89%, and ofB. bovisbetween 27% and 83%. The RLB assay was comparatively less sensitive in this study and detection ofB. bovisranged from 0% to 17%, andB. bigeminawas not detected at all by this technique. Analysis of new sequences of the 18S rRNA gene revealed that the currentB. bigeminaRLB probe is not specific for the identification of isolates in Mozambique. The RLB assay, however, resulted in the detection of 8 other haemoparasite species belonging to the generaBabesia,Theileria,AnaplasmaandEhrlichia. 18S rRNA gene sequences from theTheileriaspp. were identified, and a phylogenic tree constructed with these sequences yielded a heterogeneousT. mutans-like group. In conclusion, infection withB. bigeminaandB. bovisis endemic in Maputo Province, but rates of transmission vary. Furthermore, mixed infections with the haemoparasites responsible for several tick-borne diseases in cattle are common in Mozambique.