Asparagus is a key global vegetable crop with significant economic importance. Purple asparagus, rich in anthocyanins, stands out for its nutritional value. Despite its prominence, the molecular mechanisms driving purple peel coloration in asparagus remain unclear. This study focuses on three asparagus varieties with distinct peel colors to analyze anthocyanins in both the metabolome and transcriptome, unraveling the regulatory mechanisms. Our findings identify 30 anthocyanins, categorized into five major anthocyanin aglycones across diverse asparagus peel colors. Notably, among the 30 differentially expressed metabolites (DEMs), 18 anthocyanins displayed significantly up-regulated expression in the 'Purple Passion' variety. Key contributors include Cyanidin-3-O-rutinoside-5-O-glucoside and Cyanidin-3-O-sophoroside. Cyanidin-3-O-glucoside is most abundant in 'Purple Passion', while Petunidin-glucoside-galactoside is the least. Analysis of differentially expressed genes (DEGs) displayed 21 structural genes in anthocyanin synthesis, with F3H, DFR, ANS, and one of three UFGTs showing significantly higher expression in the 'Purple Passion' compared to 'Grande' and 'Erasmus'. Additionally, transcription factors (TFs), including 38 MYB, 33 bHLH, and 13 bZIP, also display differential expression in this variety. Validation through real-time qPCR supports the idea that increased expression of anthocyanin structural genes contribute to anthocyanin accumulation. Transient overexpression of AoMYB17 in tobacco further showed that it had the vital function of increasing anthocyanin content. This study sheds light on the mechanisms behind anthocyanin coloration in three distinct asparagus peels. Therefore, it lays the foundation for potential genetic enhancements, aiming to develop new purple-fleshed asparagus germplasms with heightened anthocyanin content.

Botrytis cinerea is a pathogenic fungus that causes gray mold disease in a broad range of crops. The high intraspecific variability of B. cinerea makes control of this fungus very difficult. Here, we isolated a variant B05.10^M strain from wild-type B05.10. The B05.10^M strain showed serious defects in mycelial growth, spore and sclerotia production, and virulence. Using whole-genome resequencing and site-directed mutagenesis, a single nucleotide mutation in the adenylate cyclase (BAC) gene that results in an amino acid residue (from serine to proline, S1407P) was shown to be the cause of various defects in the B05.10^M strain. When we further investigated the effect of S1407 on BAC function, the S1407P mutation in bac showed decreased accumulation of intracellular cyclic AMP (cAMP), and the growth defect could be partially restored by exogenous cAMP, indicating that the S1407P mutation reduced the enzyme activity of BAC. Moreover, the S1407P mutation exhibited decreased spore germination rate and infection cushion formation, and increased sensitivity to cell wall stress, which closely related to fungal development and virulence. Taken together, our study indicates that the S1407 site of bac plays an important role in vegetative growth, sclerotial formation, conidiation and virulence in B. cinerea.

To meet the large demand for Asparagus officinalis in the spring market and improve the economic benefits of cultivating asparagus, we explored the molecular mechanism underlying the response of A. officinalis to low temperature. First, “Fengdao No. 1” was screened out under low-temperature treatment. Then, the transcriptome sequencing and hormone detection of “Fengdao No. 1” and “Grande” (control) were performed. Transcriptome sequencing resulted in screening out key candidate genes, while hormone analysis indicated that ABA was important for the response to low temperature. The combined analysis indicated that the AoMYB56 gene may regulate ABA in A. officinalis under low temperature. And the phylogenetic tree was constructed, and subcellular localisation was performed. From these results, we speculated that the AoMYB56 gene may regulate ABA in A. officinalis. The results of this research provide a theoretical basis for the further exploration of low-temperature response in A. officinalis.

Soluble sugar content is a key determinant of taste and flavor in radish taproots, particularly differences in sucrose content. To elucidate the regulatory mechanisms underlying sucrose accumulation in radish taproots, targeted sugar metabolomics and transcriptome analyses were performed using 'QX' (high soluble sugar and high sucrose content) and 'HG' (low soluble sugar and low sucrose content) at the mature taproot stage (90 days after sowing). Targeted metabolomics analysis identified a total of eight differentially expressed metabolites (DEMs), including five upregulated and three downregulated metabolites. Transcriptome analysis revealed 5265 upregulated genes and 5539 downregulated genes. RT-qPCR analysis displayed that there were multiple differentially expressed pivotal genes and transcription factors of sucrose metabolism. Integrated multi-omics analysis indicated that differentially expressed genes (DEGs) were predominantly enriched in fructose and mannose metabolism, galactose metabolism, and starch and sucrose metabolism pathways. Further yeast one-hybrid screening demonstrated that the RsNF-YA6 specifically binds to RsSUS2. Dual-luciferase reporter assays indicated that RsNF-YA6 significantly suppressed RsSUS2 expression, confirming its role as a negative regulatory transcription factor localized in the nucleus. The overexpression of RsNF-YA6 in Arabidopsis and radish led to a remarkable elevation in sucrose content. Collectively, these results reveal the regulatory function of the RsNF-YA6-RsSUS2 module in radish sucrose metabolism, offer novel insights into the molecular mechanisms underlying sucrose accumulation in radish taproots, and provide important genetic resources and a theoretical basis for breeding high-sucrose radish varieties.