Staphylococcus aureus is a Gram-positive bacterium and one of the most prevalent infectious disease-related causes of morbidity and mortality in adults. This pathogen can trigger a broad spectrum of diseases, from sepsis and pneumonia to severe skin infections that can be fatal. In this review, we will provide an overview of S. aureus and discuss the extensive literature on epidemiology, transmission, genetic diversity, evolution and antibiotic resistance strains, particularly methicillin resistant S. aureus (MRSA). While many different virulence factors that S. aureus produces have been investigated as therapeutic targets, this review examines recent nanotechnology approaches, which employ materials with atomic or molecular dimensions and are being used to diagnose, treat, or eliminate the activity of S. aureus. Finally, having a deeper understanding and clearer grasp of the roles and contributions of S. aureus determinants, antibiotic resistance, and nanotechnology will aid us in developing anti-virulence strategies to combat the growing scarcity of effective antibiotics against S. aureus.

Chitosan is known to exert antimicrobial activity without the need for any chemical modification; however, new derivatives of chitosan can be created to target multi-drug resistant bacteria. In this study, chitosan (CS) was cross-linked with sodium tripolyphosphate to form nanoparticles, which were then coated with polyacrylic acid (PAA). The SEM images revealed that the CS-PAA nanoparticles had spherical shapes with smooth surfaces and the size of the dried nanoparticles was approximately 222 nm. Biofilm formation was significantly inhibited by 0.5 mg/mL of CS-PAA. In-situ optical microscopy showed that CS-PAA nanoparticles inhibited the bacterial biofilm formation in Campylobacter jejuni, Pseudomonas aeruginosa, and Escherichia coli after a single treatment with 40 μg. Additionally, 20 µg of CS-PAA nanoparticles demonstrated antibacterial activity against the growth of C. jejuni, P. aeruginosa, and E. coli with notable inhibitory zones of 9, 12, and 13 mm, respectively (P < 0.01). The development of a novel and ecofriendly method for the preparation of chitosan nanoparticles through an interaction of chitosan with PAA shows promise tool to combat bacterial infections and validates effective antibacterial and antibiofilm properties against antibiotic resistant pathogens.

Nanosized ceria (CeO₂) and a graphitic carbon nitride-loaded ceria (CeO₂/GCN) nanocomposite were synthesized using a straightforward and efficient method and characterized by XRD, FTIR, SEM, TEM, TGA, and BET analyses. These techniques confirmed that CeO₂ was effectively supported on the surface of GCN, with particle sizes of the CeO₂/GCN composite in the range of 10–15 nm and a pore size of 3.33 nm. The photocatalytic activity of the CeO₂/GCN nanocomposite and CeO₂ NPs in the degradation of methyl red dye under sunlight radiation was studied using UV–visible spectroscopy. A noticeable red shift in the CeO₂/GCN nanocomposite compared to pure CeO₂ NPs suggests a reduction in its band gap energy, calculated at 3.90 eV for CeO₂ NPs and 2.97 eV for the CeO₂/GCN nanocomposite. This band gap reduction enhances the photocatalytic degradation process, achieving a removal efficiency of 99.92% within a short irradiation time of 40 min for the CeO₂/GCN nanocomposite, compared to 69.47% for CeO₂ NPs. These findings indicate that graphitic carbon nitride significantly enhances the photocatalytic properties of CeO₂ NPs.

In this investigation, the chemical composition of the hydro-distilled essential oil (HD-EO), obtained from the fresh aerial parts (inflorescence heads (Inf), leaves (L), and stems (St)) of Conyza canadensis growing wild in Jordan was determined by GC/MS. Additionally, the methanolic extract obtained from the whole aerial parts of C. canadensis (CCM) was examined for its total phenolic content (TPC), total flavonoids content (TFC), DPPH radical scavenging activity, iron chelating activity and was then analyzed with LC-MS/MS for the presence of certain selected phenolic compounds and flavonoids. The GC/MS analysis of CCHD-EOs obtained from the different aerial parts revealed the presence of (2E, 8Z)-matricaria ester as the main component, amounting to 15.4% (Inf), 60.7% (L), and 31.6% (St) of the total content. Oxygenated monoterpenes were the main class of volatile compounds detected in the Inf-CCHD-EO. However, oils obtained from the leaves and stems were rich in polyacetylene derivatives. The evaluation of the CCM extract showed a richness in phenolic content (95.59 ± 0.40 mg GAE/g extract), flavonoids contents (467.0 ± 10.5 mg QE/ g extract), moderate DPPH radical scavenging power (IC₅₀ of 23.75 ± 0.86 µg/mL) and low iron chelating activity (IC₅₀ = 5396.07 ± 15.05 µg/mL). The LC-MS/MS profiling of the CCM extract allowed for the detection of twenty-five phenolic compounds and flavonoids. Results revealed that the CCM extract contained high concentration levels of rosmarinic acid (1441.1 mg/kg plant), in addition to caffeic acid phenethyl ester (231.8 mg/kg plant). An antimicrobial activity assessment of the CCM extract against a set of Gram-positive and Gram-negative bacteria, in addition to two other fungal species including Candida and Cryptococcus, showed significant antibacterial activity of the extract against S. aureus with MIC value of 3.125 µg/mL. The current study is the first phytochemical screening for the essential oil and methanolic extract composition of C. canadensis growing in Jordan, its antioxidant and antimicrobial activity.

Introduction: The beneficial effects of probiotics are encountered by their low viability in gastrointestinal conditions and their insufficient stability during manufacturing, throughut the gastrointestinal transit, and storage. Therefore, novel systems are highly required to improve probiotics delivery. Methods: In this study, Lactobacillus gasseri (L), Bifidobacterium bifidum (B), and a combination of L+B were encapsulated in chitosan (CS)-polyacrylic acid (PAA) complex systems (CS-PAA). The CS-PAA systems were analysed on basis of morphology, size, and zeta potential. The loaded CS-PAA systems were evaluated for their morphology, particle size, zetapotential, vaiability in both simulated gastroic and intestinal fluids, and stability at 4°C storage temparature. Moreover, the antibiofilm activity of the probiotics-loaded systems were evaluated againt Campylobacter jejuni, Pseudomonas aeruginosa and Escherichia coli. Results: Probiotic strains were successfully incorporated into the porous structures of the CS-PAA systems, either individually or in combination. The Loaded L, B, and L+B showed higher particle size than the unloaded particles and excellent viability in simulated gastric and intestinal fluids, where the free probiotic species were undetected. Additionally the loaded probiotic exhibited an anti-biofilm effect at 0.5 mg/mL concentration level. Conclusion: The CS-PAA complexes demonstrate a promising mechanism for the effective delivery of incorporated probiotics. The probiotics exhibited high viability and maintain stability under physiological conditions, and showed a remarkable anti-biofilm activity. These characteristics suggest that CS-PAA could serve as an alternative system for probiotics, enhancing gut microbiota health and offering a robust protection against microbial pathogens.

Influenza is a frequent infectious disease that can be prevented and is associated with significant mortality and morbidity. The most economical way to prevent influenza is through vaccination, although this method is not widely used. This study aimed to assess the seasonal influenza vaccination rates and the knowledge and attitudes of Jordanian adults with chronic illnesses toward the influenza vaccine. A cross-sectional design was employed. A 26-item online survey was used to gather data about patients' knowledge of and attitudes toward the influenza vaccine as well as their status as influenza vaccine recipients. A total of 19% of the 564 study participants received an influenza vaccination. Most (81%) of individuals reported inconsistent vaccination uptake. The most important factor affecting vaccination is the belief that the flu is not a threat (39%) and that their doctors did not advise them about the vaccination (32%). Participants with no health insurance and those with public insurance had a lower level of vaccination than those with private insurance (p= 0.008). The adult population of Jordan with chronic diseases has subpar immunization rates. What is also revealed is a blatant misunderstanding about the value of routine influenza vaccination. These findings emphasize how urgently the public needs to be made aware of the effectiveness of the influenza vaccine.