Grazing intensity is one of the crucial anthropogenic activities on alpine grasslands. However, how grazing intensity affects soil microorganism diversities and their co-occurrence networks in alpine steppe remains uncertain. We carried out a controlled grazing experiment (null grazing, CK; moderate grazing, MG; and heavy grazing, HG) on a typical alpine steppe in the Lhasa River Basin, Central Tibet, China. We used high-throughput sequencing to find the sequences of bacterial 16S rRNA and fungal ITS gene amplicons. Then, we analyzed their alpha and beta diversities and set up co-occurrence networks that show how often they occur together. MG significantly increased the bacterial Shannon index and changed the bacterial community structure. In contrast, HG decreased the fungal ACE and Chao1 indices and also changed the fungal community structure (p < 0.05). Linear mixed-effect model revealed that available phosphorus in soil significantly impacted on soil bacterial Shannon, ACE, and Chao1 indices across grazing intensities, while total carbon in subsoil significantly affected these indicators of soil fungi. Moreover, MG increased the complexity of the co-occurrence network in the bacterial community, while HG simplified it. However, both MG and HG made the co-occurrence networks in the fungal community less complicated. This shows that the intensity of grazing has different impacts on how microbes interact with each other. Therefore, sustainable grazing intensity necessitates a deeper understanding of biodiversity conservation in alpine grasslands.

Poverty eradication is the first goal on the United Nations’ list of 17 Sustainable Development Goals (SDGs). However, the relationships between poverty eradication and the other SDGs remain unclear, and thus current knowledge is insufficient to support the synergized achievement of the SDGs. China eliminated extreme poverty in 2021, and thus in this study, we analyzed the variations in sustainability and equality related to the environment and the provision of public services in China during the poverty eradication program from 2010 to 2020. We combined statistical data, point of interest data, and environmental monitoring and remote sensing data to evaluate progress in China’s 832 poverty-stricken counties. The results showed that the mean values of environmental and public service sustainability indicators improved from 2010 to 2020. In addition, the mean Theil index value decreased from 0.46 to 0.35 during this period, implying a reduction in inequality. Inequality between poverty-stricken and non-poverty-stricken areas accounted for 9.3% of overall inequality among the counties in 2010, and this had decreased to 7.7% in 2020. However, unbalanced regional investment resulted in increased gaps between poverty-stricken and non-poverty-stricken counties in relation to education and health care. Overall, 15% of the population and 54.8% of the land area in poverty-stricken counties experienced both a loss of their advantage in terms of environmental quality compared with non-poverty-stricken counties and greater lags in the provision of public services. Linear estimation showed that investment in poverty-stricken counties should be increased by 226.2 and 72.0% in relation to education and health care, respectively, to eliminate these inequalities.

Microbial community development is a crucial aspect of soil restoration. The employment of frame beams in conjunction with external soil has demonstrated efficacy in the rehabilitation of degraded roadside ecosystems within mining regions. Nonetheless, the effects of frame beams on the composition and stability of soil bacterial communities remain inadequately comprehended. We conducted a one-time soil sampling on a three-year restored slope in a large-scale metal mining area on the Tibetan Plateau, providing a snapshot of the current conditions and evaluating the restoration progress. Frame beams with external soil covers were applied at three different altitudes: A1 (4800-5000 m), A2 (4500-4700 m), and A3 (4200-4400 m). Restoration significantly altered bacterial community composition compared with controls. Proteobacteria had a higher relative abundance in the restoration area (average: 31.16 %), whereas Acidobacteriota were more abundant in the control area (average: 24.68 %). In the restoration area, soil bacterial α-diversity increased as elevation decreased, with the Shannon index rising from 5.34 (A1) to 5.82 (A3), suggesting that bacterial communities at higher altitudes are more sensitive to environmental conditions. Species turnover was the primary driving factor of β-diversity, accounting for 96.26 % under A1, 94.71 % under A2, and 91.94 % under A3, respectively. The nearest taxon index of bacterial communities shifted from negative to positive along the elevation gradient (-0.25 to 1.14), indicating an increasing trend toward community clustering. Within the bacterial co-occurrence network, soil pH and total phosphorus contribute significantly to network strength, closeness, and betweenness. Concluding, soil pH and total phosphorus were identified as key factors shaping bacterial diversity and assembly mechanisms. Our research contributes to the development of effective soil restoration strategies for alpine mining regions, providing insights into microbial community assembly and stability mechanisms.